Yazar "Yilmaz-Dilsiz O." seçeneğine göre listele

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    The detrimental effects of diabetes on pluripotency determined by Klf4, Sox2, c-Myc and Oct4 immunoreactivity in rat testes
    (2013) Aktug H.; Uysal A.; Yavasoglu A.; Oltulu F.; Akarca S.O.; Yilmaz-Dilsiz O.; Oktem G.
    Background. Diabetes mellitus (DM) is a multisystem disorder. Type 1 DM can be experimentally induced in rats with streptozotocin (STZ). Diabetic conditions result in testicular oxidative stress and suppressed male reproductive activity as well as decreases in both testicular organ weights and subject weights. Objectives. The purpose of this study was to investigate immunohistochemical differences in testicular tissue due to STZ induced diabetes regarding pluripotency via transcription factors like Klf4, Sox2, c-Myc and Oct4, and to determine weight changes in both the subjects and the testes during the experiment. Material and Methods. Diabetes was induced in male adult rats for this study. A healthy control group and a diabetic group were observed for one month. Blood glucose levels over 250 mg/dL were considered diabetic. Results. On days 0, 3, 15 and 30, the subjects' weights and testicular organ weights were determined and analyzed. The results revealed statistically significant decreases (p < 0.05 and p < 0.001, respectively). Semiquantitative immunohistochemical analyses of Klf4, Sox2, c-Myc and Oct4 were studied in testes paraffin sections via light microscopy. Decreased immunoreactivity of Klf4 was observed in the diabetic group in comparison to the controls. Spermatogonial cells and Sertoli cells snowed increased immunostaining for Sox2 and c-Myc, while decreased immunoreactivity of Oct4 was noted for both spermatogenic and Sertoli cells compared to the control group. Conclusions. This study clearly demonstrated that Klf4, Sox2 and Oct4 immunopositive cells in adult male rat testes manifested sustainable pluripotency and that diabetes has dramatically detrimental effects on this trait. © Copyright by Wroclaw Medical University.
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    The effects of different intraabdominal pressure protocols in laparoscopic procedures on oxidative stress markers and morphology in rat ovaries
    (Wroclaw University of Medicine, 2014) Biler A.; Yucebilgin S.; Sendag F.; Akman L.; Akdemir A.; Ates U.; Uyanikgil Y.; Yilmaz-Dilsiz O.; Sezer E.
    Background: To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. Objectives: The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. Material and Methods: Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Ppl5, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. Results: In Groups Pp10 and Ppl5, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Ppl5 than in Groups Pp10 and IPp15. Conclusions: Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than lowpressure pneumoperitoneum methods.
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    Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin
    (2012) Akarca S.O.; Yavaşog A.; Ayşegül U.; Fatih O.; Yilmaz-Dilsiz O.; Timur K.; Hüseyin A.
    This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N010) and diabetes groups (group 2, N010). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson's Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P00.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson's Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. © Research Society for Study of Diabetes in India 2012.