Yazar "Ucar, Fusun" seçeneğine göre listele

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    Identification by multiplex PCR of phylogeny and papG alleles in uropathogenic E. coli strains
    (Current Biology Ltd, 2011) Giray, Betul; Ucar, Fusun
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    ISOLATION AND IDENTIFICATION OF COPPER AND NICKEL TOLERANT ASCOMYCETOUS YEASTS IN TURKEY
    (Parlar Scientific Publications (P S P), 2011) Gungor, Orcun; Ucar, Fusun; Yalcin, H. Tansel; Corbaci, Cengiz; Akpinar, Onur
    Twenty yeast strains were isolated from environments containing different heavy metals and all of these isolates were identified by phenotypic tests. Sixteen isolates detected to be higher in metal tolerance were also identified using the genotypic test (5,8S ITS PCR). Moreover, PCR sequence analysis fragments, which were obtained from ITS-PCR, were accomplished. The phenotypically identified isolates were eight different species belonging to six genera and including four isolates which were not identified by genotypic identification. According to the genotypic identification, the most widely available species was Yarrowia lipolytica (62.5%). Other identified genera were Pichia, Candida and Galactomyces. All the similarities identified as result of sequence analysis were > 90% with the NCBI GenBank. Two strains (strain numbers 14 and 19) were found to be highest tolerant to Cu2+ and Ni2+, respectively, and these results were correlated with findings of the dry weight.
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    Molecular characterization of Yarrowia lipolytica strains isolated from different environments and their lipase production profiles
    (Current Biology Ltd, 2011) Akpinar, Onur; Ucar, Fusun; Yalcin, Husniye Tansel
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    Screening and regulation of alkaline extracellular protease and ribonuclease production of Yarrowia lipolytica strains isolated and identified from different cheeses in Turkey
    (Springer, 2011) Akpinar, Onur; Ucar, Fusun; Yalcin, H. Tansel
    In this research, 43 yeast strains were isolated from different types of cheese in Turkey and these isolates were identified with both phenotypic tests and genotypic tests with 5.8S ITS-PCR. Also, the sequence of PCR fragments obtained from ITS-PCR was analyzed by DNA sequencing. In the results of the phenotypic tests, 9 of 43 isolates were identified as Yarrowia lipolytica. After the phenotypic identification, all the isolates were identified with ITS-PCR and 13 were identified as Y. lipolytica. Yarrowia lipolytica secretes several hydrolytic enzymes including alkaline and acid proteases, lipases, a ribonuclease, and phosphotases. In this paper, the effect of carbon and nitrogen sources and pH on production of AEP and RNase were examined for our identified strains of Y. lipolytica. The best production of AEP were found in skim milk medium with pH 7.0 by TEM YL 5 and Y. lipolytica CBS 6124 type strain. An extra carbon source (glucose) reduced AEP production at all the pH studied. Glutamine which is a nitrogen source did not affect AEP production. In contrast to AEP production, glucose increased RNase production at all the pH studied.