ISOLATION AND IDENTIFICATION OF COPPER AND NICKEL TOLERANT ASCOMYCETOUS YEASTS IN TURKEY
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Twenty yeast strains were isolated from environments containing different heavy metals and all of these isolates were identified by phenotypic tests. Sixteen isolates detected to be higher in metal tolerance were also identified using the genotypic test (5,8S ITS PCR). Moreover, PCR sequence analysis fragments, which were obtained from ITS-PCR, were accomplished. The phenotypically identified isolates were eight different species belonging to six genera and including four isolates which were not identified by genotypic identification. According to the genotypic identification, the most widely available species was Yarrowia lipolytica (62.5%). Other identified genera were Pichia, Candida and Galactomyces. All the similarities identified as result of sequence analysis were > 90% with the NCBI GenBank. Two strains (strain numbers 14 and 19) were found to be highest tolerant to Cu2+ and Ni2+, respectively, and these results were correlated with findings of the dry weight.
Açıklama
Anahtar Kelimeler
yeasts, heavy metal tolerance, identification, ITS-PCR
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
20
Sayı
1A
