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Öğe Antioxidant Activities of Silver and Iron Nanoparticles of Psephellus pyrrhoblepharus (Boiss) Wagenitz(Innovhub Ssi-Area Ssog, 2021) Tastan, Pelin; Kivcak, BijenInterest in nanotechnology is increasing day by day. Silver and iron nanoparticles of the chloroform extract of Psephellus pyrrhoblepharus were prepared for this study by green synthesis method. The characterisation of the nanoparticles was carried out with some methods like the Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), X-ray Diffraction Analysis (XRD), zeta potential and particle size. The antioxidant activities and total phenolic capacities of nanoparticles and three different extracts of P. pyrrhoblepharus (chloroform, methanol:water (1:1) and n-hexane) were compared. Silver nanoparticles were the most active ones by antioxidant activities and total phenolic capacity. Although the iron nanoparticles showed activity, they did not show a successful activity as silver nanoparticles. This research is essential because it contains the first nanoparticle studies prepared from the plant P. pyrrhoblepharus.Öğe Assessment of antioxidant and neuroprotective activity of plants from the Lamiaceae family(Istanbul Universitesi, 2024) Fafal, Tugce; Kayalar, Husniye; Birim, Dervis; Armagan, Guliz; Tuzun, Burcu Sumer; Tastan, Pelin; Kivcak, BijenBackground and Aims: Plants from the Lamiaceae family are shown to have pharmacological activities, including antiinflammatory, antidiabetic, antiasthmatic, hypolipidemic, antibacterial. This research measured the antioxidant and neuroprotective capacity of methanol extracts from the Mattubium vulgate L., Phlomis atmeniaca Willd., Thymus haussknechtii Velen. and, Thymus kotschyanus Boiss. & Hohen plants in cellular and cell-free systems. Methods: The neuroprotective potential of Mattubium vulgate, Phlomis atmeniaca, Thymus haussknechtii, and T. kotschyanus were determined against H2O2 toxicity in SH-SY5Y, the human neuroblastoma cell line. The antioxidant capacity of methanol extracts was examined by radical scavenging assays using static exempt chemical groups, 2.2'-diphenyl-1-picrylhydrazyl-hydrate (DPPH) and 2.2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Folin-Ciocalteau and Aluminum chloride assays were used to measure phenolic and flavonoid contents of the extracts. Results: Following these experiments, the effect of three different concentrations of extracts (1, 10 and 100 mu g/mL) on cell viability was assessed by a WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium)]. The methanol extract of Mattubium vulgate was found to have the highest antiradical activity against DPPH (76.439 +/- 0.42%). The Thymus kotschyanus (10 mu g/mL) protected cells from H2O2-induced toxicity in all the extracts (p<0.05). Conclusion: In our report, we suggest that Thymus kotschyanus have potential neuroprotective activity because of the existence of polyphenolic compounds, flavonoids, and phenolic acids.Öğe Comparing the Fatty Acid Composition and Neuroprotective Effects of Some Lamiaceae Taxa from Turkey(Springer, 2022) Fafal, Tugce; Kaftan, Gizem; Tastan, Pelin; Kivcak, Bijen; Armagan, GulizThis study aims to analyze the fatty acid composition and neuroprotective potential of some selected Lamiaceae taxa from Turkey. For this purpose, methanol extracts of Lamium album L., Lamium garganicum L., Phlomis rigida Labill., Salvia syriaca L., Salvia sclarea L., and Stachys cretica subsp. anatolica Rech. f. were investigated for their activity against hydrogen peroxide induced neuronal cell death. The fatty acid compositions were analyzed by GC-FID, and the IA and IT indices and the omega 3/omega 6 ratio were also calculated. Saturated and unsaturated fatty acids of selected Lamiaceae species were measured to range from 8.92-17.59% and 75.13-84.57%, respectively. Phlomis rigida with the highest omega 3/omega 6 was shown to have the highest neuroprotection against H2O2-induced cell toxicity.Öğe Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract(Marmara Univ, 2023) Tastan, Pelin; Fafal, Tugce; Tuezuen, Burcu Suemer; Ozmen Yelken, Besra; Kayabasi, Cagla; Yilmaz Susluer, Sunde; Gunduz, CumhurThe aim of the study is to examine the cytotoxic, apoptotic effects and gene expressions of Asphodelus aestivus water extract (ASP) and silver nanoparticles (AgNPS) on decided cancer lines. Breast cancer cell lines MCF-7 and MDA-231; melanoma cancer lines MEWO and CHL-1; fibroblast cancer lines WI-38 and HEL 299 were selected for biological activities. xCELLigence system was used for cytotoxicity. Annexin V-EG FP Apoptosis detection kit was used for apoptosis and gene expressions were assessed by real time online RT-PCR by using cancer cell lines Qiagen kits. AgNPS showed significant cytotoxicity in all cell lines. The most prominent apoptosis was determined in MCF-7 cell line for AgNPS. It has been observed that the most important progress in gene expression, the suppression capacity of MUC1-C, a breast cancer-associated oncoprotein, is greatly increased by nanoparticle formation. In addition, cells that were not affected at all in MDA-MB-231 breast cancer oncoprotein started to suppress with nanoparticles. In conclusion, it was determined that silver nanoparticles increased the effect especially in breast cancer cell lines and could be considered for use.Öğe Evaluation of cell protection by Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage(Taylor & Francis Ltd, 2022) Tastan, Pelin; Armagan, Guliz; Dagci, Taner; Kivcak, BijenNeurodegenerative diseases affect millions of people. Major reasons behind the onset and progression of these diseases are still under investigation. Therefore, any approach that would treat/prevent progression is important. In this study, we aimed to investigate the potential protective effects of Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage and compare the effectiveness of different extracts (methanol:water (1:1), chloroform and n-hexane). The cells were pretreated with four different concentrations (10, 50, 100, and 200 mu g/ml) of methanol:water (1:1), chloroform and n-hexane extracts of P. pyrrhoblepharus following MPP+ treatment for 12 or 24 h. The changes in cell viability were determined using the MTT assay. Additionally, antioxidant activities and total phenolic/flavonoid contents of the extracts were determined with radical scavenging capacity, Folin-Ciocalteu and aluminum chloride assays, respectively. The extracts at selected concentrations were found to be protective in a dose-dependent manner at 12 and 24 h. Nevertheless, the methanol extract of the plant showed the highest protection both at 100 and 200 mu g/ml (115.13%+/- 3.98, 121.87%+/- 1.66; p < 0.05) against dopaminergic damage at 24 h. The results showed that selected concentrations were not toxic and did not affect cell proliferation rate. Besides, the chloroform extract was found to have higher antioxidant activity than the other extracts (p < 0.05). The total phenolic and total flavonoid contents were found consistent with antioxidant activities. Our findings support the neuroprotective and antioxidant potential of P. pyrrhoblepharus. However, further studies on identifying the presence of chemicals in P. pyrrhoblepharus extracts which are responsible for protection should be carried out to confirm their therapeutic potential.Öğe Evaluation of cell protection by Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage(Taylor & Francis Ltd, 2019) Tastan, Pelin; Armagan, Guliz; Dagci, Taner; Kivcak, BijenNeurodegenerative diseases affect millions of people. Major reasons behind the onset and progression of these diseases are still under investigation. Therefore, any approach that would treat/prevent progression is important. In this study, we aimed to investigate the potential protective effects of Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage and compare the effectiveness of different extracts (methanol:water (1:1), chloroform and n-hexane). The cells were pretreated with four different concentrations (10, 50, 100, and 200 mu g/ml) of methanol:water (1:1), chloroform and n-hexane extracts of P. pyrrhoblepharus following MPP+ treatment for 12 or 24 h. The changes in cell viability were determined using the MTT assay. Additionally, antioxidant activities and total phenolic/flavonoid contents of the extracts were determined with radical scavenging capacity, Folin-Ciocalteu and aluminum chloride assays, respectively. The extracts at selected concentrations were found to be protective in a dose-dependent manner at 12 and 24 h. Nevertheless, the methanol extract of the plant showed the highest protection both at 100 and 200 mu g/ml (115.13%+/- 3.98, 121.87%+/- 1.66; p < 0.05) against dopaminergic damage at 24 h. The results showed that selected concentrations were not toxic and did not affect cell proliferation rate. Besides, the chloroform extract was found to have higher antioxidant activity than the other extracts (p < 0.05). The total phenolic and total flavonoid contents were found consistent with antioxidant activities. Our findings support the neuroprotective and antioxidant potential of P. pyrrhoblepharus. However, further studies on identifying the presence of chemicals in P. pyrrhoblepharus extracts which are responsible for protection should be carried out to confirm their therapeutic potential.Öğe FATTY ACID COMPOSITION AND ANTIOXIDANT ACTIVITY OF Ranunculus constantinopolitanus(Springer, 2022) Say, Deniz; Tastan, Pelin; Fafal, Tugce[No Abstract Available]Öğe Fatty acids and antioxidant capacities of three Centaurea L. species(Marmara Univ, 2022) Tastan, Pelin; Tuzun, Burcu; Kivcak, Bijen; Fafal, TugceThe aim of this study is to determine the fatty acid compositions and antioxidant activities of three Centaurea species as Centaurea iberica, C. urvillei ssp. hayekiana, and C. urvillei ssp. nimrodis and compare them with each other. As a result of fatty acid determination by GC MS method, oleic acid and linoleic acid were found as the main fatty acids in these Centaurea species. Polyunsaturated fatty acids (PUFAs) were determined at 58.66%, 46.98%, 62.22% in C. iberica, C. urvillei ssp. hayekiana and C. urvillei ssp. nimrodis, respectively. Antioxidant activities of methanolic extracts of three Centaurea species were evaluated by DPPH, ABTS and CUPRAC assays. The methanol extract of C. urvillei ssp hayekiana showed the highest antioxidant activity in direct proportion to the total amount of phenolic and flavonoid substances. The fact that the antioxidant activities of these three species and fatty acid analyzes have not been compared in previous studies is important in that this study is the first.Öğe Fatty Acids Compositions of Some Lamiaceae Plants(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Tastan, Pelin; Birim, Dervis; Fafal, Tuğce; Armağan, Güliz; Kıvçak, BijenThe Lamiaceae family is a large family that includes many genera and species. In this study fatty acid compositions of the plants Stachys lavandulifolia, Salvia verticillata, Phlomis herba venti sp. pungens and Phlomis sieheana were determined by using GC/MS method. Accordingly, it was determined that the ratio of monounsaturated fatty acids varied between 18-34% and the ratio of polyunsaturated fatty acids between 24-49%. Besides, the major saturated fatty acids found for each plant were tricosanoic acid and lignoceric acid, while the major unsaturated fatty acids were determined as nervonic acid, eicodadienoic acid and docosadienoic acid. Fats have many beneficial effects on health, especially as a high energy source. Thanks to its high content of unsaturated fatty acids, it is supported that plants have a positive effect on health. The results obtained will shed light on each study to be made with these plants.Öğe Fear of Covid-19: Associations with Trait Anxiety and Life Satisfaction(Lahore Medical Research Center Llp, 2021) Isik, Utku; Ustun, Neslisah Aktas; Tastan, Pelin; Ustun, Umit DoganAim: Present study aimed to analyze perceived fear of COVID-19 among Turkish people and investigate its associations with trait anxiety and life satisfaction. Methods: The online snowball research sampling method was used to collect data, and 1129 subjects participated in the study. As data collection tools, the Fear of COVID-19 scale, Trait Anxiety Scale, and Satisfaction with Life Scale were used. In the adaptation and validation process of the Fear of COVID-19 Scale, confirmatory factor analysis was applied. Convergent validity of the model resulting from CFA was evaluated with Average Variance Extracted (AVE) and Composite Reliability (CR) based on the factor load values. The reliability of the scale was assessed with Cronbach's alpha method. Independent samples t-test and Two-Way ANOVA analysis were used to compare the differences in the perception of fear of COVID-19, trait anxiety and life satisfaction. Multiple regression was applied to predict anxiety based on fear of COVID-19 and life satisfaction. Results: Analyze results confirmed the unidimensionality of the 7-item scale on a Turkish sample, and the results indicated satisfactory reliability coefficients. According to independent t-test and Two Way Anova results, females, non-athletes, participants with the worry of gaining weight, who did not care about their nutrition, and who followed media news more often scored higher fear of COVID-19 and anxiety. Multiple regression analysis identified a negative correlation between fear of COVID-19 and life satisfaction and a positive correlation with anxiety. Conclusion: Although the present study revealed the associations of the fear of Covid-19 with life satisfaction and anxiety, further research is still recommended to explore these associations in more depth.Öğe Phlomis species protects the cells from oxidative stress by upregulating catalase enzyme expression(Innovhub Ssi-Area Ssog, 2022) Tastan, Pelin; Birim, Dervis; Fafal, Tugce; Armagan, Guliz; Kivcak, BijenRecently, interest in the pharmacological effects of plants has increased and studies on bioactive compounds have intensified. In this study, we aimed at evaluating the antioxidant and neuroprotective potential of Lamiaceae plants including Stachys lavandulifolia, Salvia verticillata, Phlomis herba venti sp. pungens and Phlomis sieheana in both cellfree systems and the cell culture model. Methanol extracts of the aerial parts of these plants, cultivated in Elazig, Turkey were carried out. Antioxidant activity assays (ABTS and DPPH) and total phenolic and flavonoid contents of the extracts were measured. Next, the effects of methanol extracts on cell viability and catalase protein levels were investigated in hydrogen peroxide induced oxidative stress model. DPPH radical scavenging activities were determined with the IC50 value of 31.36 +/- 0.25, 30.44 +/- 0.19, 30.16 +/- 0.08 and 56.54 +/- 0.93 for Stachys lavandulifolia, Salvia verticillata, Phlomis herba venti sp. pungens and Phlomis sieheana, respectively. ABTS radical scavenging activities were determined as 6.38 +/- 1.48, 16.93 +/- 0.68, 5.97 +/- 4.04 and 2.28 +/- 0.51. The highest cellular protection and catalase expression were determined in Phlomis-treated cells against H2O2 (p<0.05). On the other hand, no statistically significant change in cell viability was observed when the extracts were compared with each other. Our findings support the neuroprotective and antioxidant potential of these four Lamiaceae plants, particularly Phlomis species.Öğe Sesquiterpene Lactones and Flavonoids from Psephellus pyrrhoblepharus with Antiproliferative Activity on Human Gynecological Cancer Cell Lines(Mdpi, 2019) Tastan, Pelin; Hajdu, Zsuzsanna; Kusz, Norbert; Zupko, Istvan; Sinka, Izabella; Kivcak, Bijen; Hohmann, JuditMultistep chromatographic separations of the chloroform extract of the Turkish endemic plant Psephellus pyrrhoblepharus (Boiss.) Wagenitz (syn. Centaurea pyrrhoblephara Boiss.) resulted in the isolation of six guaianolid-type sesquiterpenes, chlorojanerin (1), 19-deoxychlorojanerin (2), 15-hydroxyjanerin (3), aguerin B (4), cynaropicrin (5), eleganin (6); three flavonoids, apigenin, 6-methoxyluteolin and jaceosidine; two glycosides, benzyl-1-O-beta-D-glucoside and 3(Z)-hexenyl-1-O-beta-D-glucoside; and the coumarin scopoletin. the structures were established by the interpretation of their ESI-MS and 1D and 2D NMR data including H-1-NMR, JMOD, H-1, H-1-COSY, HSQC, HMBC, and NOESY experiments. All compounds were isolated for the first time from P. pyrrhoblepharus. Compounds 1-6, the isolated flavonoids and scopoletin were evaluated for their antiproliferative activities on human gynecological cancer cell lines (SiHa, HeLa, and MDA-MB-231 cells) using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Chlorojanerin (1), 19-deoxychlorojanerin (2), aguerin B (4), cynaropicrin (5), eleganin (6) were shown to have noteworthy effects on all of the tested cell lines, while apigenin, jaceosidine, and 6-methoxyluteolin were moderately active on HeLa cells. the highest activities were demonstrated by the chlorine-containing derivatives chlorojanerin (1) and 19-deoxychlorojanerin (2) with IC50 values of 2.21 and 2.88 mu M, respectively, against the triple negative breast cancer model MDA-MB-231 cells.Öğe Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract(Walter De Gruyter Gmbh, 2020) Tuzun, Burcu Sumer; Fafal, Tugce; Tastan, Pelin; Kivcak, Bijen; Yelken, Besra Ozmen; Kayabasi, Cagla; Gunduz, CumhurASP was used to synthesize FeNPA. They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. the aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNPA and ASP. the antioxidant properties were evaluated using DPPH, ABTS(+) and H2O2 assays. FeNPA had higher antioxidant activity comparing to ASP according to DPPH (IC50: 3.48 mu g/mL) and ABTS(+) (60.52%) assays. Anti-cancer activities of FeNPA and ASP were investigated in breast cancer, melanoma and control cell lines. FeNPA was more cytotoxic than ASP in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNPA had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. ASP also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in ASP, and FeNPA treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in ASP and FeNPA administration. It had been shown that FeNPA regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNPA are also evaluated in animal models in vivo.
