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Öğe BIOCONVERSION OF WINE POMACE BY LENTINUS EDODES IN A SOLID-STATE SYSTEM(Slovak Univ Agriculture Nitra, 2021) Yildirim, Hatice Kalkan; Sozmen, Eser Y.Wine pomace is a by-product of wine industry. The objective of this investigation was to evaluate the biotransformation of wine pomace by L. edodes MCC 55. As substrates were used wine pomaces of Ozbek and Gaziantep wines produced under conventional conditions. L.edobes MCC 55 cultures were used for biotransformation. During study were determined the effects of different media used during solid state fermentation (water and CaCO3, NH4NO3) and different solvents used during extraction (water and ethanol) on phenols and consequently their radical scavenging activities. The ethyl alcohol used during extraction stages was determined as more effective solvent regarding antioxidant activity (TEAC) values. Using of water both during fermentation and extraction steps was determined to be significant for increasing the values of hydroxycinnamic and hydroxybenzoic acids. There were determined close relations among total phenol, tartaric acid esters, flavonols, anthocyanins and gallic acid values with samples produced by solid state fermentation with additional nutrient and using ethyl alcohol during extraction stage. Close interaction between antioxidant activity (TEAC) values and samples obtained by using ethanol during extraction were indicated, also. These results demonstrated that by-products such as wine pomace could be successfully used for production of valuable bioactive compounds.Öğe Biotransformation of propolis phenols by L-plantarum as a strategy for reduction of allergens(Korean Society Food Science & Technology-Kosfost, 2018) Yildirim, Hatice Kalkan; Canbay, Erhan; Ozturk, Sahin; Aldemir, Ozan; Sozmen, Eser Y.Propolis is a natural product produced by honeybees. It has antioxidant effects as well as antimicrobial, antiseptic, antibacterial, anti-inflammatory and antimutagenic properties. Except these important healthy properties some cytotoxic effects causing allergies also have been reported. In this study have been evaluated changes of phenolic compounds including allergens molecules found in propolis. Before biotransformation, propolis samples were treated with different solvent (ethanol and polyethylene glycol) to facilitate solvation of solid samples. Biotransformation was done by three different strains of Lactobacillus plantarum (10, 8014, ATCC). Results demonstrated the importance of used solvent/treatment for extraction procedure and strains of L. plantrum. The lowest values of main allergens were determined as 321ng/mL for BCAFE, 320ng/mL for 1.1 DMAECAFE and 8.02ng/mL for CAPE. The study is the first work deal with evaluation of bioconversion of propolis by different L. plantarum strains and their effects on phenolic profile.Öğe The effect of nesting positions on pain, stress and comfort during heel lance in premature infants(Elsevier Taiwan, 2018) Kahraman, Ayse; Basbakkal, Zumrut; Yalaz, Mehmet; Sozmen, Eser Y.Background: Nesting positions are commonly used in procedural analgesic administration in premature neonates. The effectiveness of nesting positions is questioned. The aim of the this study was to assess the pain, stress, comfort and salivary cortisol and melatonin values in nesting positions during the heel lance procedure in premature infants at the NICU. Methods: Experimental research; repeated measurement design. The sample comprised 33 premature neonates with gestational age of 31-35 weeks who had been hospitalized in the NICU. Nesting positions were given using linen or towels. The procedure of heel lance was recorded on camera. The camera recordings were evaluated according to the NIPS and the COMFORTneo scale. Saliva samples were obtained five minutes prior to and 30 min after the heel lance procedure. Salivary Cortisol and Melatonin were measured using the Salimetrics Cortisol Elisa Kit and the Salimetrics Melatonin Elisa Kit. Results: The crying time, the mean NIPS score, the COMFORTneo score, the COMFORTneo NRS-pain scores and the COMFORTneo NRS-distress scores for premature neonates who were in the prone position during the procedure were significantly lower than the scores in the supine position (p < 0.000). Furthermore, the level of salivary cortisol five minutes prior to and 30 min after the heel lance procedure had significantly decreased in the prone position; however, there were insignificant differences in the mean levels of salivary melatonin between the positions. Conclusions: Nesting in the prone position has a pain reducing effect, enhancing comfort and reducing stress in premature infants. Copyright (C) 2017, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC.Öğe Effect of pheniramine maleate on rat skeletal muscle ischemia-reperfusion injury(Turkish Assoc Trauma Emergency Surgery, 2022) Erol, Kubilay; Sozmen, Eser Y.; Kucuk, Ulku; Kucuk, LeventBACKGROUND: Skeletal muscle ischemia-reperfusion injury (IRI) is a common clinical problem encountered after tourniquet application or replantation. This study investigated the effect of pheniramine maleate (Ph), which is frequently used in clinical practice to reduce IRI, and compared its efficacy in IRI with N-acetylcysteine (NAC), a molecule that has been shown to be effective in IRI. METHODS: Twenty-eight male Sprague-Dawley rats were randomly divided into four groups (sham, ischemia-reperfusion [IR], IR+Ph, IR+NAC; n=7 rats per group). Ischemia was induced in the lower right extremities of rats for 3 h using a femoral artery clamp and an elastic tourniquet. Ph and NAC were administered intraperitoneally 15 min before ischemia was terminated. At 24 h after reperfusion, levels of thiobarbituric acid reactive substance (TBARS), catalase (CAT), myeloperoxidase (MPO), superoxide dismutase (SOD), polyadenosine diphosphate ribose polymerase (PARP), and neutrophil infiltration were evaluated. Inducible nitric oxide synthase (iNOS) density in muscle tissue was evaluated by immunohistochemical methods after 1 week. RESULTS: SOD, MPO, PARP, CAT, and TBARS levels in muscle tissue were significantly lower in the sham group compared with the other groups (p<0.001). All parameters except TBARS were lower in the NAC and Ph groups than in the IR group (p<0.001). Neutrophil infiltration in the muscle tissue samples from the IR group was significantly increased compared with the NAC and Ph groups (p<0.05). iNOS staining was not observed in the sham and NAC groups. CONCLUSION: Ph is effective at reducing experimental rat skeletal muscle IRI.Öğe Effect of PPAR-gamma agonists on macrophage activation in type2 diabetes mellitus(Derman Medical Publ, 2018) Sozmen, Bulent; Karakas, Emel Yigit; Topcugil, Fusun; Altiner, Nazif; Sozmen, Eser Y.Aim: Recently, it has been proposed that inflammation triggered by macrophages as the pathogenic mechanism linked to the development of obesity-related insulin resistance. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonists which increase the insulin receptor sensitivity. might improve glycemic control by enhancing insulin sensitivity and ameliorate the impaired lipid profile. In this study, we assessed the effect of rosiglitazone (PPAR-gamma agonist) on the insulin resistance and inflammatory markers. Material and Method: Thirty patients (11 men. 19 women) with type II diabetes mellitus (DM) were taken into the study. They were treated by rosiglitazone in a dose of 4mg/day as well as a diet for 12 weeks. Results: Rosiglitazone treatment significantly decreased HbA1c (p<0,01), IR-HOMA, hsCRP (p<0,01), triglyceride levels (p<0,05), CETP activity (p<0,01) and basal serum oxidation (TBARS levels, p<0,05). However, Chitotriosidase activity significantly increased after Rosiglitazone treatment (p<0,01). TNF, IL-6, sTNFR1, and sTNFR2 levels showed no statistically significant difference compared to their basal levels. Discussion: Rosiglitazone might treat diabetes by mediating glucose/lipid metabolism and preventing lipid oxidation in patients with DM. On the other hand, it has a dual role on inflammation; while it might induce macrophage activation suggesting its pro-inflammatory effect, It might also reduce the CRP levels by lowering gene expression suggesting its anti-inflammatory effect.Öğe Effect of Red Wine Consumption on Serum Oxidation and Adiponectin Levels in Overweight and Healthy Individuals(De Gruyter Open Ltd, 2014) Karadeniz, Muammer; Akcay, Yasemin D.; Yildirim, Hatice K.; Yilmaz, Candeger; Sozmen, Eser Y.Obesity is a well-known independent risk factor for CVD and metabolic syndrome. It has been shown that moderate red wine consumption might reduce the risk of cardiovascular disease (CVD). The aim of this study was to investigate whether red wine has anti-inflammatory and antioxidative effect in overweight subjects. Ten overweight and 14 healthy subjects drank 200 mL/day of red wine for one month. While the Cabernet Sauvignon wine caused a significant increase in paraoxonase activity (p<0.05), it led to a decrease in basal and stimulated LDL diene levels in both of the groups (p<0.05). Total antioxidant activity increased in the two groups following wine consumption. While wine consumption had no effect on IL-6, TNF-alpha and CRP levels of overweight subjects, it led to an increase in adiponectin levels. Current data consider that regular red wine consumption may be beneficial to improve the antioxidant potential of LDL oxidation in overweight patients. In addition to the prevention of LDL oxidation, moderate wine consumption might delay the onset of atherosclerosis through an increase in paraoxonase and adiponectin levels.Öğe False positive diagnosis of lysosomal disease with dried blood spot (DBS) sample; leukocyte number as a challenging factor(Academic Press Inc Elsevier Science, 2017) Sozmen, Eser Y.; Dondurmaci, Meral; Ucar, Sema Kalkan; Coker, MahmutÖğe Higher levels of CD19(+) leukocytes in Gaucher disease patients as a potential marker for malignancy(Derman Medical Publ, 2018) Dondurmaci, Meral; Canda, Ebru; Kose, Melis; Ucar, Sema Kalkan; Coker, Mahmut; Sagin, Ferhan; Sozmen, Eser Y.Aim: Gaucher disease is an autosomal recessive lysosomal storage disease caused by insufficient glucocerebrosidase activity resulting in accumulation of glucosylceramide, particularly in macrophages. Multiple myeloma and B cell lymphoma are considered to be one of the causes of death from GD in the long term. We aimed to compare cell surface antigens of leukocytes to try to identify a reliable marker for leukocyte infiltration and progression to lymphoid malignancy. Material ve Method: 10 Gaucher disease patients and 20 age-matched healthy controls were included. Leukocytes were collected from whole blood using a Ficoll gradient, stained for specific cell surface antigens (CD33, CD19, CD14, and CD8) and sorted by flow cytometry. Levels of each leukocyte cell surface antigen were expressed as a percentage of leukocytes expressing them. Leukocyte glucocerebrosidase activity was measured by fluorometry. Results: The percentage of CD19+ leukocytes in Gaucher disease patients (8.2 +/- 3.4) was significantly higher than in the control group (4.8 +/- 3.4) (p < 0.05). The percentage of leukocytes expressing CD33 (12.8 +/- 6.6 vs 7.9 +/- 8.0, p=0.077), CD14 (10.6 +/- 4.6 vs 7.1 +/- 6.9, p=0.094) or CD8 (12.7 +/- 5.3 vs 9.8 +/- 5.9, p=0.115) was not significantly higher in patients than in controls. Discussion: The higher levels of CD19+ leukocytes may serve as a useful marker to predict leukocyte infiltration and perhaps also malignancy in Gaucher disease patients. Experimental anti-CD19 drugs are in development for the treatment of B cell cancers, and CD19+ leukocyte levels may also serve as a marker of the response to this treatment.Öğe Is D-dimer used according to clinical algorithms in the diagnostic work-up of patients with suspicion of venous thromboembolism? A study in six European countries(Pergamon-Elsevier Science Ltd, 2016) Kristoffersen, Ann Helen; Ajzner, Eva; Rogic, Dunja; Sozmen, Eser Y.; Carraro, Paolo; Faria, Ana Paula; Watine, Joseph; Meijer, Piet; Sandberg, SverreIntroduction: Clinical algorithms consisting of pre-test probability estimation and D-dimer testing are recommended in diagnostic work-up for suspected venous thromboembolism (VTE). The aim of this study was to explore how physicians working in emergency departments investigated patients suspected to have VTE. Materials and methods: A questionnaire with two case histories related to the diagnosis of suspected pulmonary embolism (PE) (Case A) and deep venous thrombosis (DVT) (Case B) were sent to physicians in six European countries. The physicians were asked to estimate pre-test probability of VTE, and indicate their clinical actions. Results: In total, 487 physicians were included. Sixty percent assessed pre-test probability of PE to be high in Case A, but 7% would still request only D-dimer and 11% would exclude PE if D-dimer was negative, which could be hazardous. Besides imaging, a D-dimer test was requested by 41%, which is a "waste of resources" (extra costs and efforts, no clinical benefit). For Case B, 92% assessed pre-test probability of DVT to be low. Correctly, only D-dimer was requested by 66% of the physicians, while 26% requested imaging, alone or in addition to D-dimer, which is a "waste of resources". Conclusions: These results should encourage scientific societies to improve the dissemination and knowledge of the current recommendations for the diagnosis of VTE. (C) 2016 Elsevier Ltd. All rights reserved.Öğe Leukocyte cell surface antigens in Gaucher disease: New implications for B-cell proliferation and pathogenesis of myelomatosis(Academic Press Inc Elsevier Science, 2015) Sozmen, Eser Y.; Dondurmaci, Meral; Ucar, Sema Kalkan; Sagin, Ferhan Girgin; Coker, MahmutÖğe The Levels of Glutathione and Nitrite-Nitrate and the Expression of BCL-2 MRNA in Ovariectomized Rats Treated by Raloxifene Against Kainic Acid(Taylor & Francis Ltd, 2009) Armagan, Guliz; Kanit, Lutfiye; Terek, Cosan M.; Sozmen, Eser Y.; Yalcin, AyferThe selective estrogen receptor modulators (SERMs) are compounds that activate the estrogen receptors with different estrogenic and antiestrogenic tissue-specific effects. The similar effects of SERMs on estrogen encourage the efforts in the research of neuroprotective effects of SERMs. In our study, the potential neuroprotective effects of raloxifene were investigated on the brain cortex of ovariectomized rats after kainic acid-induced oxidative stress. To show the neuroprotective effect of raloxifene against a neurodegenerative agent, kainic acid, expression of Bcl-2, total glutathione (GSH), and nitrite-nitrate levels were investigated in the rat brain cortex. Our results demostrate that raloxifene treatment against oxidative stress significantly increases the expression of Bcl-2 and the level of GSH in the brain cortex.Öğe Misunderstandings about paraoxonase-1(Assoc Brasileira Otorrinolaringologia & Cirurgia Cervicofacial, 2022) Mackness, Michael; Sozmen, Eser Y.[No Abstract Available]Öğe Patients with Gaucher type 1: Switching from imiglucerase to miglustat therapy(Academic Press Inc Elsevier Science, 2018) Canda, Ebru; Kose, Melis; Kagnici, Mehtap; Ucar, Sema Kalkan; Sozmen, Eser Y.; Coker, MahmutÖğe PON192 Polymorphism and LDL-Oxidation As Risk Factors For Coronary Artery Disease in Young Turkish Population(Walter De Gruyter Gmbh, 2009) Sozmen, Eser Y.; Kayikcioglu, Meral; Sezer, Ebru; Ilanbey, Bilal; Akcay, Yasemin D.; Kultursay, HakanPurpose: There are conflicting reports on paraoxonase1 (PON1) polymorphism as a risk factor for cardiovascular disease. It's been proposed that there is relationship between coronary artery disease and PON1-55L or PON1-192R alleles. Nevertheless recent publications showed major differences of PON1 activity and concentration in different populations. We aimed to study low-density-lipoprotein (LDL) oxidation and PON1 activities (paraoxonase/arylesterase) regarding to PON192 polymorphism, in order to determine if they might be useful markers especially for young patients with coronary artery disease in Turkish population. Methods: 60 patients (38.1 +/- 5.0 years) with coronary artery disease and 52 healthy control subjects (32.5 +/- 6.1 years) were taken into study. Paraoxonase192 polymorphism, paraoxonase activity and oxidation status of all patients were analyzed. Results: Total cholesterol, triglyceride, basal-LDL-diene and stimulated-LDL-TBARS levels were higher, paraoxonase and arylesterase activities were lower in patients with coronary artery disease compared to controls(p<0.001). While there was no difference in any parameters within control group in regard to polymorphism, stimulated-LDL TBARS levels were higher in patients with RR polymorphism (5,2 +/- 2,4 nmol/mg LDL protein for RR; 3,64 +/- 1.28 nmol/mg LDL protein for QR and 4,95 +/- 2,8 nmol/mg LDL protein for QQ). RR polymorphism was more common in patient group than controls but not statistically significant. Conclusion: Our data suggest that presence of RR polymorphism might be a predictive marker for determination of atherosclerosis in early ages.Öğe Pre- and post-test probabilities of venous thromboembolism and diagnostic accuracy of D-dimer, estimated by European clinicians working in emergency departments(Pergamon-Elsevier Science Ltd, 2017) Kristoffersen, Ann Helen; Ajzner, Eva; Miquel Bauca, Josep; Carraro, Paolo; Faria, Ana Paula; Hillarp, Andreas; Rogic, Dunja; Sozmen, Eser Y.; Watine, Joseph; Meijer, Piet; Sandberg, SverreÖğe PROTECTIVE EFFECTS OF BLUEBERRY ON CCL4 INDUCED ACUTE HEPATOTOXICITY IN RATS(Walter De Gruyter & Co, 2011) Guner, Gulin; Yilmaz, Funda; Yildirim, Hatice Kalkan; Sozmen, Eser Y.; Akcay, Yasemin DelenÖğe Reference intervals of alpha-glycosidase, beta-glycosidase, and alpha-galactosidase in dried blood spot in a Turkish newborn population(Springer, 2013) Aldemir, Ozan; Ergun, Pelin; Gunes, Sezgin; Koroglu, Ozge Altun; Yalaz, Mehmet; Kultursay, Nilgun; Coker, Mahmut; Sozmen, Eser Y.Inherited lysosomal storage diseases (LSDs) are rare, and diagnosis is often delayed for 7-10 years. Since the therapies have become available for a limited number of LSDs, (Fabry, Gaucher, Pompe, and MPS-1), early diagnosis of treatable LSDs can be lifesaving or ameliorating and allows timely treatment before irreversible damage occurs. Recently, the use of dried blood spot test (DBS) for newborn screening of LSDs has been proposed for newborn screening tests. They are noninvasive, sensitive, and specific assays with the further advantage of a fast turnaround time compared to measurement in leukocyte and/or fibroblast culture. We aimed to determine the reference intervals for lysosomal enzyme activities of newborn babies in our population and to investigate the effect of gestational week on enzyme activity. One hundred thirty healthy newborn babies (70 girls, 60 boys) were included into the study. alpha-Glycosidase, beta-glycosidase, and alpha-galactosidase activities in DBS samples of newborns were determined fluorometrically. Reference intervals were calculated using Dixon's rule and percentiles of 2.5-97.5. Cutoff limits (5 %) for alpha-glycosidase, beta-glycosidase, and alpha-galactosidase activities were 0.57, 0.92, and 2.18, respectively. alpha-Galactosidase activity was higher in girls compared to boys (p < 0.05). Interestingly, alpha-glycosidase and beta-glycosidase activities of newborns who were delivered before 38 weeks were significantly lower than those who were delivered at 39-40 weeks. Conclusion It is of utmost importance to define the reference intervals for lysosomal enzyme activities as well as cutoff limits for newborn babies with regard to gestational age and sex. More studies to clarify the reason for the change in enzyme activity by gestational week will be required.Öğe Role of total antioxidant capacity and oxidative stress in fertilization and embryo selection in the IVF cycle(Cukurova Univ, Fac Medicine, 2021) Seyfettinoglu, Sevtap; Sahin, Gulnaz; Akdogan, Aysin; Goker, Ege Nazan Tavmergen; Akcay, Yasemin; Sozmen, Eser Y.; Tavmergen, ErolPurpose: This study aims to evaluate the oxidant and antioxidant parameters in follicular fluid and embryo culture medium in IVF cycles and investigate their effects on embryo quality, fertilization success, and pregnancy. Materials and Methods: Fifty-one patients who underwent IVF/ICSI were included in this prospective study. Two hundred sixty of a total of 454 follicular fluids and the cell culture media of transferred fifty-one embryos (one from each patient) were examined. Antioxidant activity (AOA), Trolox equivalent antioxidant capacity (TEAC), thiobarbiturate reactive substances (TBARS), ferric antioxidant power (FRAP), paraoxonase, nitrotyrosine were analyzed. Results: TBARS were significantly lower in the follicular fluid of fertilized oocytes than in non-fertilized oocytes (21 vs. 29.75 nmol/ml). There was no difference in terms of other parameters. TEAC levels were higher in oocytes in the agonist protocol group than in the antagonist protocol group. In terms of embryo quality, there were no differences between the groups in any analysis. TEAC levels were higher in the embryo cell culture medium in the group that became pregnant after embryo transfer on the second day. Conclusion: When oxidative stress and antioxidant parameters were evaluated in both follicle fluid and embryo cell culture medium, it was determined that lipid peroxidation negatively affected fertilization. However, there is no significant difference in terms of embryo quality and pregnancy rates.Öğe Roles of Cytokines in Pathological and Physiological Gastroesophageal Reflux Exposure(Korean Soc Neurogastroenterology & Motility, 2024) Ergun, Pelin; Kipcak, Sezgi; Gunel, Nur S.; Bor, Serhat; Sozmen, Eser Y.Background/Aims Gastroesophageal reflux disease is frequently observed and has no definitive treatment. There are 2 main views on the pathogenesis of gastroesophageal reflux disease. The first is that epithelial damage starts from the mucosa by acidic-peptic damage and the inflammatory response of granulocytes. The other view is that T-lymphocytes attract chemoattractants from the basal layer to the mucosa, and granulocytes do not migrate until damage occurs. We aim to investigate the inflammatory processes occurring in the esophageal epithelium of the phenotypes at the molecular level. We also examined the effects of these changes on tissue integrity. Methods Patients with mild and severe erosive reflux, nonerosive reflux, reflux hypersensitivity, and functional heartburn were included. Inflammatory gene expressions (JAK/STAT Signaling and NFKappaB Primer Libraries), chemokine protein levels, and tissue integrity were examined in the esophageal biopsies. Results There was chronic inflammation in the severe erosion group, the acute response was also triggered. In the mild erosion group, these 2 processes worked together, but homeostatic cytokines were also secreted. In nonerosive groups, T-lymphocytes were more dominant. In addition, the inflammatory response was highly triggered in the reflux hypersensitivity and functional heartburn groups, and it was associated with physiological reflux exposure and sensitivity. Conclusions Microinflammation in physiological acid exposure groups indicates that even a mild trigger is sufficient for the initiation and progression of inflammatory activity. Additionally, the anti-inflammatory cytokines were highly increased. The results may have a potential role in the treatment of heartburn symptoms and healing of the mucosa. (J Neurogastroenterol Motil 2024;30:290-302)Öğe Sample blank subtraction outreachs hemoglobin interferences in flurorometric methods for DBS(Academic Press Inc Elsevier Science, 2012) Sozmen, Eser Y.
