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    Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients
    (Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo, 2018) Susluer, Sunde Yilmaz; Kayabasi, Cagla; Yelken, Besra Ozmen; Asik, Aycan; Celik, Didem; Okcanoglu, Tugce Balci; Senger, Suheyla Serin; Avci, Cigir Biray; Kose, Sukran; Gunduz, Cumhur
    Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes, including epigenetic regulation, cell-cycle control, and transcriptional/translational regulation of gene expression. Differential expression of lncRNAs and disruption of the regulatory processes are recognized as critical steps in cancer development. The role of lncRNAs in hepatitis B virus (HBV) infection is not well understood. Here we analyzed the expression of 135 lncRNAs in plasma samples of 82 HBV patients (classified as chronic patients, inactive carriers, or resolved patients) at diagnosis and at 12 months of treatment in relation to control group (81 healthy volunteers). We also investigated the effect of small interfering RNA (siRNA)-mediated silencing of lincRNA-SFMBT2 on HBV-positive human liver cancer cell line. lncRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. We observed changes in lncRNA expression in all three HBV groups, compared to control group. Most notably, the expression of anti-NOS2A, lincRNA-SFMBT2, and Zfhx2as was significantly increased and expression of Y5 lncRNA was decreased in chronic HBV patients. A decreased Y5 expression and increased lincRNA-SFMBT2 expression were observed in inactive HBsAg carriers. The expression of HOTTIP, MEG9, and PCAT-32 was increased in resolved HBV patients, and no significant change in the expression of Y5 was observed, compared to control group. siRNA-mediated inhibition of lincRNA-SFMBT2 decreased the level of HBV DNA in human liver cancer cells. Further research is needed to confirm the prognostic as well as therapeutic role of these lncRNAs in HBV patients.
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    Expression profile alterations of lncRNAs on the molecular pathogenesis of hepatitis B
    (Wiley, 2016) Susluer, Sunde Yilmaz; Kose, Sukran; Kayabasi, Cagla; Yelken, Besra Ozmen; Asik, Aycan; Celik, Didem; Senger, Suheyla Serin; Avci, Cigir Biray; Gunduz, Cumhur
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    Prevalence of allergic diseases in Izmir Province, Turkey
    (Wiley-Blackwell, 2014) Kose, Sukran; Mandiracioglu, Aliye; Cavdar, Gulsun; Ulu, Yildiz; Senger, Suheyla Serin
    Background The aim of the present study is to determine the prevalence of allergic diseases and related risk factors in Izmir, Turkey, and to assess the positive immunoglobulin E (IgE) levels against common allergens. Methods The questionnaires were completed via face-to-face interview with a total of 2937 individuals. Specific IgE was studied in the serum samples by enzyme-linked immunosorbent assay (ELISA). This cross-sectional population-based study comprised adolescents over the age of 15 years and adults of Izmir province. Results In Izmir, the prevalence of self-reported allergy was 35.5%. Allergy was statistically significantly more common in females, university graduates, in those with high income, with family history for allergy, aged 40-49 years, those living in a flat, keeping pet(s), and in those using central heating. The prevalence of atopy according to the serum IgE levels (Class 3 with any of 3 IgEs) was found 1.6%. Conclusion In conclusion, the prevalence of atopy was found lower in Izmir as compared to the results of other studies.
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    Seroprevalence of varicella-zoster virus in the prevaccine era: A population-based study in Izmir, Turkey
    (Elsevier Science London, 2013) Kose, Sukran; Mandiracioglu, Aliye; Senger, Suheyla Serin; Ulu, Yildiz; Cavdar, Gulsun; Gol, Basak; Gurbuz, Ilhan; Sariavci, Senol; Nohutcu, Nese
    Purpose: To determine the population-based seroprevalence of varicella zoster virus (VZV) and related risk factors in Izmir. Materials and methods: A population-based household survey was conducted. A representative sample of the population older than 15 years of age was selected using multistage random sampling. A total of 2136 healthy persons participated in this cross-sectional study. The participants completed a questionnaire designed to collect data related to socio-demographic characteristics and risk factors. Blood samples were collected, and VZV-specific IgG was measured using an ELISA. Results: In total, 94.3% of individuals were seropositive for VZV. The difference between VZV seroprevalence in urban and rural populations was significant (OR: 2.6 (95% CI, 1.7-3.8)). No statistically significant differences in seropositivity were observed with respect to other sociodemographic characteristics. Conclusion: A large proportion of the participants were found to be immune to VZV. (C) 2012 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.