DSpace Arşivi :: by Yazar "Karavana, Sinem Yaprak" değerine göre listeleniyor

Yazar "Karavana, Sinem Yaprak" seçeneğine göre listele

Listeleniyor 1 - 20 / 55

Antifungal etkili maddelerin biyoadezif vajinal ilaç şekilleri üzerine çalışmalar
(Ege Üniversitesi, 2010) Baloğlu, Esra; Karavana, Sinem Yaprak
Bioadhesive system, gel, in-situ gel, thiolated polymer, miconazole nitrate, econazole nitrate.;Biyoadezif sistem, jel, in-situ gel, tiyole polimer, mikonazol nitrat, ekonazol nitrat.;Vajinal kandidiyazis, çok sık görülen ve kadınların %75'inin yasamları boyunca en az bir kere karsılastıkları bir rahatsızlıktır. Bu projenin hedefi, vajinal kandidiyazis tedavisinde kullanılmak üzere imidazol türevi olan ekonazol nitrat ve mikonazol nitrat ile farklı biyoadezif formülasyonlar gelistirmektir. Bu amaç için kitosan, poloksamerler ve tiyole polimer kullanılarak sırasıyla jel, in situ jel ve tablet formülasyonları hazırlandı. Öncelikle, farklı molekül agırlına sahip kitosanlar ile jel formülasyonunları hazırlandı ve jellerin pH'sı, mekanik özellikleri, akıs özellikleri ve salım profilleri incelendi. Kitosanın, ekonazol nitrat ve mikonazol nitrat biyoadezif jel formülasyonları için kullanılabilecegi ve bu tip formülasyonların vajinal uygulamaları için yararlı olacagı görüldü. Çalısmanın ikinci kısmında, poloksamer karısımlarından olusan in situ vajinal jel formülasyonları hazırlandı. Hazırlanan formülasyonların mekanik, reolojik ve salım özellikleri degerlendirildi ve in vivo sartlarda Candida albicans ATCC 10231'e karsı formülasyonun antifungal aktivitesi arastırıldı. Sonuçlara dayanılarak ekonazol nitrat ve mikonazol nitratın %20 Poloxamer 407: %10 Poloxamer 188 karısımı ile hazırlanan in situ jel formülasyonunun, tek dozluk uygulamayla vajinal kandidiyazis tedavisinde kullanılmak üzere etkin ve umut vaad edici oldugu saptandı. Son olarak, tiyole Poliakrilik asit-sistein polimeri ile biyoadezif vajinal tabletler hazırlandı. Tabletlerin USP XXX'a göre agırlık sapması, friabilite, sertlik ve çap/kalınlık oranları incelendi. Formülasyonların sisme kapasiteleri ve mukoadezif özellikleri de degerlendirildi. Ekonazol nitrat ve mikonazol nitratın modifiye edilmemis poliakrilik asit ve tiyole poliakrilik asit- sistein tabletlerinden salım profillerinde anlamlı bir fark gözlendi. Ayrıca, tiyole polimer ile 152 saate kadar kontrollü salım saglandı. Tüm sonuçlar degerlendirildiginde tiyole tablet formülasyonunun Candida vajinitlerinin topikal tedavisi için ümit vaad eden bir aday oldugu anlasıldı.
Bioadhesiv benzidamin hidroklorür formülasyonlarının hazırlanması ve in vitro-in vivo değerlendirilmesi
(Ege Üniversitesi, 2007) Karavana, Sinem Yaprak; Ertan, Gökhan; Güneri, Pelin
Çalışmamızda etkin madde olarak seçilen benzidamin hidroklorür; antiinflamatuvar, lokal anestezik, antipiretik, analjezik etki gösteren sistemik ve topikal olarak uygulanabilen nonsteroidal antiinflamatuvar bir ilaçtır. Oral ülser tedavisinde kullanılan birçok farklı tipte preparatı Türkiye İlaç Piyasası’nda bulunmasına rağmen biyoadezif özellikte bir preparatı bulunmamaktadır. Bu nedenle, çalışmamızda değişik hidroksipropilmetil sellüloz (HPMC) tipleri (E 5, E15, E 50 ve K 100 M) farklı oranlarda kullanılarak benzidamin hidroklorür’ün biyoadhezif özellikte preparatlarının hazırlanması amaçlanmıştır. Oral ülser tedavisinde kolay uygulanması nedeniyle jel tipi formülasyonlar tercih edilmiştir. Hazırlanan jellerde gerekli kontroller yapıldıktan sonra, in vitro çalışmaların sonuçlarına göre %2.5 HPMC K 100M ideal formülasyon seçilmiştir. Çalışmamızın ikinci kısmında, deney hayvanlarında oral ülser modeli oluşturulmuştur. İn vitro çalışmalar sonucunda ideal olarak bulunan formülasyonun oral ülser üzerindeki etkinliği, çalışmamıza özgü geliştirdiğimiz histolojik skorlama sistemiyle ve oral ülser alanının büyüklüğü takip ederek saptanmıştır. İn vivo çalışmalar, kontrol grubu, boş jel ile tedavi edilen grup ve benzidamin hidroklorür içeren jel ile tedavi edilen grup olmak üzere üç grupla gerçekleştirilmiştir. Bu şekilde hem etkin maddenin hem de biyoadezif jelin tedavideki etkinliğini araştırmak mümkün olmuştur. Çalışmamız sonucunda; etkin madde içermeyen biyoadezif jelin kendisinin de etkin madde içeren jele yakın derecede oral ülser tedavisine etkisi olduğu ortaya çıkmıştır.
Biyoadheziv benzidamin hidroklorür formülasyonlarının hazırlanması ve in vitro-in vivo değerlendirilmesi
(Ege Üniversitesi, 2007) Karavana, Sinem Yaprak; Ertan, Gökhan
Çalışmamızda etkin madde olarak seçilen benzidamin hidroklorür; antiinflamatuvar, lokal anestezik, antipiretik, analjezik etki gösteren sistemik ve topikal olarak uygulanabilen nonsteroidal antiinflamatuvar bir ilaçtır. Oral ülser tedavisinde kullanılan birçok farklı tipte preparatı Türkiye İlaç Piyasası'nda bulunmasına rağmen biyoadezif özellikte bir preparatı bulunmamaktadır. Bu nedenle, çalışmamızda değişik hidroksipropilmetil sellüloz (HPMC) tipleri (E 5, E15, E 50 ve K 100 M) farklı oranlarda kullanılarak benzidamin hidroklorür'ün biyoadhezif özellikte preparatlarının hazırlanması amaçlanmıştır. Oral ülser tedavisinde kolay uygulanması nedeniyle jel tipi formülasyonlar tercih edilmiştir. Hazırlanan jellerde gerekli kontroller yapıldıktan sonra, in vitro çalışmaların sonuçlarına göre %2.5 HPMC K 100M ideal formülasyon seçilmiştir.Çalışmamızın ikinci kısmında, deney hayvanlarında oral ülser modeli oluşturulmuştur. İn vitro çalışmalar sonucunda ideal olarak bulunan formülasyonun oral ülser üzerindeki etkinliği, çalışmamıza özgü geliştirdiğimiz histolojik skorlama sistemiyle ve oral ülser alanının büyüklüğü takip ederek saptanmıştır. İn vivo çalışmalar, kontrol grubu, boş jel ile tedavi edilen grup ve benzidamin hidroklorür içeren jel ile tedavi edilen grup olmak üzere üç grupla gerçekleştirilmiştir. Bu şekilde hem etkin maddenin hem de biyoadezif jelin tedavideki etkinliğini araştırmak mümkün olmuştur.Çalışmamız sonucunda; etkin madde içermeyen biyoadezif jelin kendisinin de etkin madde içeren jele yakın derecede oral ülser tedavisine etkisi olduğu ortaya çıkmıştır
Bukkal penetrasyonda etkin madde ve taşıyıcı etkisinin radyofarmasötiklerle incelenmesi
(Ege Üniversitesi, 2023) Karavana, Sinem Yaprak; Özdemir, Derya İlem; Ekinci, Meliha; Güneş, Meliha
Bukkal yol, yüksek hasta uyuncu, acil durumlarda dozaj formunun çıkarılabilmesi, sağlamlık ve kolay erişilebilirlik nedeniyle etkin maddelerin sistemik ve lokal etki için uygulanabilmesinde oral yola oldukça önemli bir alternatiftir. Günümüzde yapılan araştırmalar, özellikle ilk geçiş etkisine maruz kalan ilaçların bukkal uygulanan modifiye salım sistemleriyle biyoyararlanımın arttırılması gibi konulara odaklanmaktadır. In vitro penetrasyon çalışmaları, bir anatomik bölgeden etkin maddenin geçiş potansiyelini değerlendirmek için gerçekleştirilen çalışmalardır. Deri gibi, bukkal mukoza da farklı kimyasalların emilimini önlemek için fizyolojik bir bariyer görevi görür. Bukkal mukozadan etkin maddelerin geçişi pasif difüzyonla gerçekleşmektedir. Burada interselüler boşluklardan (paraselüler) difüzyon ya da intraselüler (transselüler) difüzyon söz konusudur. Hidrofilik bileşikler, nispeten hidrofilik doğasından dolayı ağırlıklı olarak paraselüler yoldan geçerken, lipofilik bileşikler transselüler yoldan geçerler. Bu projenin amacı, taşıyıcı sistemin ya da etkin maddenin özellikleri değiştirildiğinde etkin maddenin bukkal penetrasyonunun nasıl değiştiği konusunda bilgi sahibi olmaktır. In vitro permeabilite deneyleri için sığır yanak mukozası ve sentetik membran olarak selüloz asetat membran kullanılmıştır. Etkin maddeyi taklit etmek üzere 2 farklı özellikte radyofarmasötik kullanılmıştır. Bu sebeple, hidrofilik geçirgenlik modeli olarak 99mTc-DTPA ve lipofilik geçirgenlik modeli olarak ise 99mTc-HMPAO kullanılarak bukkal mukozanın in vitro geçirgenlik/permeabilite özellikleri değerlendirilmiştir. Etkin maddenin özelliğinin yanı sıra taşıyıcının da permeabiliteye olan etkisini değerlendirebilmek için 3 farklı özellikte taşıyıcı sistem (hidrojel, organojel ve bijel) hazırlanmış ve seçilen model maddelerin geçişleri bu farklı taşıyıcılar içinde incelenmiştir. Proje kapsamında planlanan çalışmalar ile bukkal yolla uygulanacak taşıyıcı sistem ya da etkin madde özellikleri değiştirildiğinde bukkal penetrasyonunun nasıl değiştiği konusunda deneyim sahibi olunmuş ve bu konuda çalışmayı planlayan araştırmacılara yol gösteren bir kılavuz sağlanmıştır.;Bukkal mukoza, Permeabilite, Hidrojel, Oleojel, Bijel, Radyofarmasötik.;Buccal mucosa, Permeability, Hydrogel, Oleogel, Bijel, Radiopharmaceutical.
A Comparative Dissolution and Histological Study of Various Vaginal Formulations Containing Ornidazole
(2009) Karavana, Sinem Yaprak; Gökçe, Evren Homan; Özyazıcı, Mine; Baloğlu, Esra; Pekcetın, Cetın; Ozyurt, Dogan; Ertan, Gokhan
…
Comparative In Vitro Release of Eletriptan Hydrobromide Formulations for Buccal Administration
(Dissolution Technologies, 2024) Ararat, Simge; Gunes, Meliha; Karavana, Sinem Yaprak
Introduction: Migraine is a neurological disease characterized by unilateral headache attacks that can last between 4-72 hours and accompanying different symptoms such as photophobia, phonophobia, osmophobia, nausea, vomiting, or movement sensitivity. Eletriptan hydrobromide (EHBR) has been recognized as a reliable and efficient treatment for severe to moderate migraine attacks, with or without aura. Buccal drug administration is the most preferred route of administration compared to other alternative routes of administration. Orally disintegrating tablets (ODT), orally disintegrating films (ODF), and in situ gel systems are popular dosage forms that can be used without the need for chewing or water when the medication is taken. With these features, they create important advantages for patients with dysphagia or problems with water intake. Methods: ODT, ODF, and in situ gel formulations were developed and evaluated in terms of dissolution profiles. Results: For the ODT formulation, more than 85% of the EHBR dissolved within the first 15 minutes. For the ODF formulation, 78% cumulative release was observed in the first 15-minutes. At the end of 4 hours, 93% cumulative drug release of EHBR from in situ gel was observed. Conclusion: Based on the results of these dissolution studies, ODT and ODF formulations for treatment of acute migraine attacks provide a rapid effect.
Design and evaluation of an intravesical delivery system for superficial bladder cancer: preparation of gemcitabine HCl-loaded chitosan-thioglycolic acid nanoparticles and comparison of chitosan/poloxamer gels as carriers
(Dove Medical Press Ltd, 2015) Senyigit, Zeynep Ay; Karavana, Sinem Yaprak; Ilem-Ozdemir, Derya; Caliskan, Cagri; Waldner, Claudia; Sen, Sait; Bernkop-Schnurch, Andreas; Baloglu, Esra
This study aimed to develop an intravesical delivery system of gemcitabine HCl for superficial bladder cancer in order to provide a controlled release profile, to prolong the residence time, and to avoid drug elimination via urination. For this aim, bioadhesive nanoparticles were prepared with thiolated chitosan (chitosan-thioglycolic acid conjugate) and were dispersed in bioadhesive chitosan gel or in an in situ gelling poloxamer formulation in order to improve intravesical residence time. In addition, nanoparticle-loaded gels were diluted with artificial urine to mimic in vivo conditions in the bladder and were characterized regarding changes in gel structure. The obtained results showed that chitosan-thioglycolic acid nanoparticles with a mean diameter of 174.5 +/- 3.762 nm and zeta potential of 32.100 +/- 0.575 mV were successfully developed via ionotropic gelation and that the encapsulation efficiency of gemcitabine HCl was nearly 20%. In vitro/ex vivo characterization studies demonstrated that both nanoparticles and nanoparticle-loaded chitosan and poloxamer gels might be alternative carriers for intravesical administration of gemcitabine HCl, prolonging its residence time in the bladder and hence improving treatment efficacy. However, when the gel formulations were diluted with artificial urine, poloxamer gels lost their in situ gelling properties at body temperature, which is in conflict with the aimed formulation property. Therefore, 2% chitosan gel formulation was found to be a more promising carrier system for intravesical administration of nanoparticles.
Design and in vitro, in vivo evaluation of antioxidant bioadhesive gelsfor burn treatment
(Tubitak Scientific & Technical Research Council Turkey, 2022) Gokce, Goksel; Karavana, Sinem Yaprak; Bagriyanik, Alper; Pekcetin, Cetin; Algin Yapar, Evren; Aybar Tural, Gulsen; Gokce, Evren Homan
Burn wounds are frequently encountered health problems, which need a new treatment approach especially in terms of good patient compliance. Availability of use of antioxidant agents and bio-adhesive gels in tissue healing can be an alternative as a new approach for wound healing. Antioxidant taurine containing bio-adhesive gels were prepared by using carbopol (CP) 940 and 934. Rheological and texture analyses were carried out on bio-adhesive gels for in vitro characterization. Wound model on Wistar rats was used to evaluate the in vivo evaluation of gels. Rheological and texture analyses showed that a carbopol bioadhesive gel has acceptable topically use dosage characteristics and in combination with Taurine it presented a successful wound healing effect via antioxidant parameters. In conclusion, bio-adhesive CP 940 (2%) gel containing 50 mM taurine could be promising in the treatment of burns by oxidative stress.
Design of Leather Footwear for Diabetics Containing Chlorhexidine Digluconate Microparticles
(Amer Leather Chemists Assoc, 2020) Yalcin, Fatih; Karavana, Huseyin Ata; Rencber, Seda; Karavana, Sinem Yaprak
ChlorHexidine Digluconate [CHD] was encapsulated inside an ethylcellulose shell material [Aquacoat ECD], and then spray dried to produce mixed microparticles (MPs). the validity and functional quality of the resultant [CHD-MPs] were analyzed on vamp and lining leather which are used to manufacture shoes for diabetics. the morphology, efficiency of encapsulation and in vitro release characteristics of the [CHD-MPs] were optimized in order to impregnate [CHD-MPs] onto leather footwear for diabetics. Scanning electron microscopy (SEM) was used to characterize the [CHD-MPs] and the leathers treated with it. SEM images illustrated that the [CHD-MPs] were spherical, smooth in shape and adhered well to leather. in vitro CHD-release studies from its MPs, and for leather treated with it were performed in phosphate buffer saline at pH =7.2. There was an inherently controlled release behaviour of CHD for all the formulations on leather. Finally, microbiologic studies on leather treated with [CHD-MPs] were done. This study suggested that footwear containing [CHD-MPs] is/will improve the quality of daily life for diabetics.
Design of orthopedic support material containing diclofenac sodium microparticles: preparation and characterization of microparticles and application to orthopedic support material
(Sage Publications Ltd, 2013) Sariisik, Merih; Arici, Mesut; Topbas, Ozlem; Karavana, Sinem Yaprak; Ozturk, Cihat; Ertan, Gokhan
The aim of this study is preparation and characterization of diclofenac sodium microparticles and their application to the orthopedic support materials. The microparticles were obtained using spray drying method involving ethyl cellulose as shell material. The morphology, particle size, drug loading capacity and in vitro release characteristics of the drug microparticles were optimized for impregnation diclofenac sodium microparticles onto the orthopedic support materials. Scanning electron microscopy (SEM) was used to characterize the drug microparticles and the treated fabrics with microparticles. SEM images illustrated that the microparticles were spherical in shape and also fixed onto the orthopedic support materials. Furthermore, the resistance of materials containing microparticles to washing were also investigated. Finally, in vitro drug release studies of microparticles and textile impregnated with microparticles were done. This study suggested that textile systems containing diclofenac sodium microparticles could have a potential for long-term therapy for rheumatic disorders.
Development and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Application
(2018) Rençber, Seda; Karavana, Sinem Yaprak
Objectives: This study aimed to prepare and evaluate mucoadhesive nanoparticle formulations of voriconazole, an antifungal drug, for mucosal application. It was also aimed to develop and validate a HPLC method of voriconazole. Materials and Methods: In this study, mucoadhesive nanoparticles containing voriconazole were prepared using a coating polymer of chitosan. The obtained nanoparticles were characterized via particle size, polydispersity index, zeta potential measurement, and mucoadhesion studies. Drug loading capacity was tested for determination of the voriconazole amount in the nanoparticles. In vitro drug release was also examined. The HPLC method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, stability, limits of detection (LOD), and limit of quantification (LOQ). Results: In vitro characterization results of the mucoadhesive nanoparticle formulation containing voriconazole was found to be appropriate with a small particle size of 217.1±4.2 nm, a narrow polydispersity index of 0.335±0.042, 99.052±0.424% drug loading, and a positive zeta potential value of +26.82±0.4 mV. According to a mucoadhesive study, it can be concluded that the nanoparticle was able to interact with mucin due to ionic interaction. Also, the turbidity of nanoparticle/mucin dispersion was higher than the turbidity of mucin dispersion itself. Based on the in vitro drug release, no burst effect was observed, indicating that voriconazole was homogeneously dispersed in the nanoparticle dispersion and that no significant amount of drug was adsorbed onto the nanoparticle surface. The release was found to follow a non?Fickian diffusion mechanism with first?order drug release. The proposed HPLC method was simple, highly sensitive with good linearity, accurate, precise, specific, and stable, showing that the method is useful for routine quality control. Conclusion: This study has shown that the mucoadhesive nanoparticle formulation containing voriconazole reported here is a promising candidate for the local treatment of mucosal diseases. The developed HPLC method can be succesfully applied to pharmaceutical preparations containing voriconazole.
Development and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Application
(2018) Rençber, Seda; Karavana, Sinem Yaprak
Objectives: This study aimed to prepare and evaluate mucoadhesive nanoparticle formulations of voriconazole, an antifungal drug, for mucosal application. It was also aimed to develop and validate a HPLC method of voriconazole. Materials and Methods: in this study, mucoadhesive nanoparticles containing voriconazole were prepared using a coating polymer of chitosan. the obtained nanoparticles were characterized via particle size, polydispersity index, zeta potential measurement, and mucoadhesion studies. Drug loading capacity was tested for determination of the voriconazole amount in the nanoparticles. in vitro drug release was also examined. the HPLC method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, stability, limits of detection (LOD), and limit of quantification (LOQ). Results: in vitro characterization results of the mucoadhesive nanoparticle formulation containing voriconazole was found to be appropriate with a small particle size of 217.1±4.2 nm, a narrow polydispersity index of 0.335±0.042, 99.052±0.424% drug loading, and a positive zeta potential value of +26.82±0.4 mV. According to a mucoadhesive study, it can be concluded that the nanoparticle was able to interact with mucin due to ionic interaction. Also, the turbidity of nanoparticle/mucin dispersion was higher than the turbidity of mucin dispersion itself. Based on the in vitro drug release, no burst effect was observed, indicating that voriconazole was homogeneously dispersed in the nanoparticle dispersion and that no significant amount of drug was adsorbed onto the nanoparticle surface. the release was found to follow a non?Fickian diffusion mechanism with first?order drug release. the proposed HPLC method was simple, highly sensitive with good linearity, accurate, precise, specific, and stable, showing that the method is useful for routine quality control. Conclusion: This study has shown that the mucoadhesive nanoparticle formulation containing voriconazole reported here is a promising candidate for the local treatment of mucosal diseases. the developed HPLC method can be succesfully applied to pharmaceutical preparations containing voriconazole.
Development and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Application
(Turkish Pharmacists Assoc, 2018) Rencber, Seda; Karavana, Sinem Yaprak
Objectives: This study aimed to prepare and evaluate mucoadhesive nanoparticle formulations of voriconazole, an antifungal drug, for mucosal application. It was also aimed to develop and validate a HPLC method of voriconazole. Materials and Methods: In this study, mucoadhesive nanoparticles containing voriconazole were prepared using a coating polymer of chitosan. The obtained nanoparticles were characterized via particle size, polydispersity index, zeta potential measurement, and mucoadhesion studies. Drug loading capacity was tested for determination of the voriconazole amount in the nanoparticles. In vitro drug release was also examined. The HPLC method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, stability, limits of detection (LOD), and limit of quantification (LOQ). Results: In vitro characterization results of the mucoadhesive nanoparticle formulation containing voriconazole was found to be appropriate with a small particle size of 217.1 +/- 4.2 nm, a narrow polydispersity index of 0.335 +/- 0.042, 99.052 +/- 0.424% drug loading, and a positive zeta potential value of +26.82 +/- 0.4 mV. According to a mucoadhesive study, it can be concluded that the nanoparticle was able to interact with mucin due to ionic interaction. Also, the turbidity of nanoparticle/mucin dispersion was higher than the turbidity of mucin dispersion itself. Based on the in vitro drug release, no burst effect was observed, indicating that voriconazole was homogeneously dispersed in the nanoparticle dispersion and that no significant amount of drug was adsorbed onto the nanoparticle surface. The release was found to follow a non-Fickian diffusion mechanism with first-order drug release. The proposed HPLC method was simple, highly sensitive with good linearity, accurate, precise, specific, and stable, showing that the method is useful for routine quality control. Conclusion: This study has shown that the mucoadhesive nanoparticle formulation containing voriconazole reported here is a promising candidate for the local treatment of mucosal diseases. The developed HPLC method can be succesfully applied to pharmaceutical preparations containing voriconazole.
Development of novel mucoadhesive gels containing nanoparticle for buccal administration of dexamethasone
(Univ Sao Paulo, Conjunto Quimicas, 2022) Rencber, Seda; Kose, Fadime Aydin; Karavana, Sinem Yaprak
This study aimed to develop promising and innovative mucoadhesive gel systems containing dexamethasone-loaded nanoparticle to increase the effectiveness of treatment for oral precancerous lesions and to reduce side effects. In this respect, a dexamethasone-loaded nanoparticle formulation was prepared by using emulsification/solvent evaporation method. The nanoparticle has high zeta potential (-10.3??0.5 mV), low particle size (218.42??2.1), low polydispersity index (0.070??0.014) and high encapsulation efficiency (95.018??2.982%). To improve the mucosal retention time, the dexamethasone-loaded nanoparticle was dispersed in mucoadhesive gel using gellan gum. The developed gels offered appropriate pH value, high drug content, suitable mechanical and mucoadhesive performance and appropriate viscosity for mucosal administration. All formulations exhibited plastic flow and typical gel-type mechanical spectra after the determined frequency value. The developed formulations exhibited extended drug release as intended for these systems. Cytotoxicity was tested by MTT assay in human epithelioid carcinoma cell (HeLa) in vitro. The MTT assay showed that the blank formulations were non-toxic to cells. It was observed that the bioactivity of the free dexamethasone was potentiated by mucoadhesive gels containing dexamethasone-loaded nanoparticle in HeLa cells. Results from this study indicate that mucoadhesive gels are effective for the local treatment of precancerous lesions. Our findings showed that the developed formulations were worthy of further studies.
Development of textile-based sodium alginate and chitosan hydrogel dressings
(Taylor & Francis As, 2020) Turkoglu, Gizem Ceylan; Sariisik, Ayse Merih; Karavana, Sinem Yaprak
Wound healing is a dynamic and complex mechanism. the healing process is affected by many factors, which can be local factors such as wound type, depth of the damaged tissue and bacterial contamination or systemic factors such as patient's age, diet, and diseases. Prior goals should be shortening the wound healing time, ease the pain of the patient and limiting the scar formation. Therefore, the wound should be evaluated profoundly, and the wound care process decided carefully. in this study, textile-hydrogel hybrids were designed to use in mild to moderate exudate wounds. Hydrogels were formed using natural based sodium alginate and chitosan polymers in different cross-linker (PEGDGE) concentrations. To identify the optimum formulation the mechanical properties (hardness, compressibility, adhesiveness, cohesiveness, and elasticity) was examined by texture profile analysis. the gel flow properties were determined by rheological analysis. the suitable formulations for dermal applications were formed on viscose and Tencel nonwovens. Physical features (mass and thickness), pH, absorbency characteristics, transfer properties (air and water vapor permeability), morphologic features (SEM), chemical properties (FT-IR) of textile-based hydrogel dressings were examined.
Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis
(Dove Medical Press Ltd, 2016) Rencber, Seda; Karavana, Sinem Yaprak; Yilmaz, Fethiye Ferda; Erac, Bayri; Nenni, Merve; Ozbal, Seda; Pekcetin, Cetin; Gurer-Orhan, Hande; Hosgor-Limoncu, Mine; Guneri, Pelin; Ertan, Gokhan
This study aimed to develop a suitable buccal mucoadhesive nanoparticle (NP) formulation containing fluconazole for the local treatment of oral candidiasis. The suitability of the prepared formulations was assessed by means of particle size (PS), polydispersity index, and zeta potential measurements, morphology analysis, mucoadhesion studies, drug entrapment efficiency (EE), in vitro drug release, and stability studies. Based on the optimum NP formulation, ex vivo drug diffusion and in vitro cytotoxicity studies were performed. Besides, evaluation of the antifungal effect of the optimum formulation was evaluated using agar diffusion method, fungicidal activity-related in vitro release study, and time-dependent fungicidal activity. The effect of the optimum NP formulation on the healing of oral candidiasis was investigated in an animal model, which was employed for the first time in this study. The zeta potential, mucoadhesion, and in vitro drug release studies of various NP formulations revealed that chitosan-coated NP formulation containing EUDRAGIT (R) RS 2.5% had superior properties than other formulations. Concerning the stability study of the selected formulation, the formulation was found to be stable for 6 months. During the ex vivo drug diffusion study, no drug was found in receptor phase, and this is an indication of local effect. The in vitro antifungal activity studies showed the in vitro efficacy of the NP against Candida albicans for an extended period. Also, the formulation had no cytotoxic effect at the tested concentration. For the in vivo experiments, infected rabbits were successfully treated with local administration of the optimum NP formulation once a day. This study has shown that the mucoadhesive NP formulation containing fluconazole is a promising candidate with once-a-day application for the local treatment of oral candidiasis.
Dexamethasone loaded PLGA nanoparticles for potential local treatment of oral precancerous lesions
(Taylor & Francis Ltd, 2020) Rencber, Seda; Kose, Fadime Aydin; Karavana, Sinem Yaprak
The aim of this study was to develop dexamethasone loaded nanoparticles for the local treatment of oral precancerous lesions. Dexamethasone loaded nanoparticles were prepared using the emulsification/solvent evaporation method. the prepared nanoparticles were characterized for pH, particle size, polydispersity index, zeta potential, morphology, encapsulation efficiency and drug loading. Furthermore, in vitro drug release, stability, ex vivo drug diffusion, and cell culture studies were undertaken. the particle size, polydispersity index, zeta potential, and encapsulation efficiency were found to be approximately 200 nm, 0.2, -10 mV, and 95%, respectively. Atomic Force Microscopy results showed that the formulated nanoparticles had uniform and spherical shape. in vitro release studies demonstrated 80% release of dexamethasone from nanoparticles; the nanoparticles were stable for 6 months. the ex vivo studies revealed no drug diffusion into the receptor media phase which suggests a possible local effect. Cytotoxicity studies showed that nanoparticles were non-cytotoxic against the HK-2 and NIH-3T3 cell lines. Findings of this study suggest that dexamethasone loaded PLGA nanoparticles are promising and can be further investigated as potential treatment of oral precancerous lesions.
Essential mint oil-based emulsions: preparation and characterization
(Inst Natl Cercetare-Dezvoltare Textile Pielarie-Bucuresti, 2019) Danila, Angela; Zaharia, Carmen; Suteu, Daniela; Muresan, Emil Ioan; Lisa, Gabriela; Karavana, Sinem Yaprak; Toprak, Ali; Popescu, Alina; Chirila, Laura
The aim of this work is to present briefly the preparation methodology of four emulsions (named M-2, M-3, M-6, and M-7) based on extracted mint oil (Mentha Piperita) and their physical-chemical properties and quality characteristics (pH, density, acidity index, peroxide index, diene and triene content, in-time stability, humidity and fatty matter content), in order to select the most recommendable emulsion to be used in textile field. This work also underlines that the most stable emulsion is M-6 emulsion followed by M-3 emulsion.
Evaluation of chitosan based vaginal bioadhesive gel formulations for antifungal drugs
(Hrvatsko Farmaceutsko Drustov (Hfd)-Croation Pharmaceutical Soc, 2014) Senyigit, Zeynep Ay; Karavana, Sinem Yaprak; Erac, Bayri; Gursel, Ozge; Limoncu, Mine Hosgor; Baloglu, Esra
The aim of the present study was to evaluate chitosan as a vaginal mucoadhesive gel base for econazole nitrate and miconazole nitrate. To this aim, different types of chitosan with different molecular masses and viscosity properties [low molecular mass chitosan (viscosity: 20,000 mPa s), medium molecular mass chitosan (viscosity: 200,000 mPa s), high molecular mass chitosan (viscosity: 800,000 mPa s)] have been used. First, rheological studies were conducted on chitosan gels. Mechanical, syringeability and mucoadhesive properties of chitosan gels were determined. Release profiles of econazole nitrate and miconazole nitrate from chitosan gels were obtained and evaluated kinetically. In addition, anticandidal activities of formulations were determined. Finally, vaginal retention of chitosan gels in rats was evaluated by in vivo distribution studies. Based on the results, it can be concluded that gels prepared with medium molecular mass chitosan might be effectively used for different antifungal agents in the treatment of vaginal candidiosis, since it has high mucoadhesiveness, suitable mechanical and release properties with good vaginal retention.
Formulation and evaluation of fluconazole loaded oral strips for local treatment of oral candidiasis
(Elsevier Science Bv, 2019) Rencber, Seda; Karavana, Sinem Yaprak; Yilmaz, Fethiye Ferda; Erac, Bayri; Nenni, Merve; Gurer-Orhan, Hande; Limoncu, Mine Hosgor; Guneri, Pelin; Ertan, Gokhan
This study aimed to develop fluconazole loaded buccal mucoadhesive oral strip for local treatment of oral candidiasis. Oral strip was prepared by solvent casting method with different polymer combinations and evaluated for weight, thickness, drug content, tensile strength, swelling behavior, in vitro disintegration time and drug release, ex vivo mucoadhesive performance, stability study, ex vivo drug diffusion and in vitro antifungal activity. Oral strips were smooth and elegant in appearance; were uniform in thickness, weight and drug content. The disintegration time was within the range of 23-28 s. The release rates of fluconazole from oral strips reached approximate to 96%. The optimum formulation was stable for 6 months. The in vitro antifungal activity studies showed the efficacy of the oral strip against Candida albicans for an extended period. Cytotoxicity was also studied, and no cytotoxic effect was observed at the tested concentration. The developed oral strip can be a promising delivery system in oral candidiasis.

Yazar "Karavana, Sinem Yaprak" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri