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Öğe Acceleration of in vitro dissolution studies of sustained release dosage form of theophylline and in vitro-in vivo evaluations in terms of correlations(Springer France, 2011) Ertan, Gokhan; Karasulu, Ercument; Ozguney, Isik; Karasulu, Yesim; Apaydin, Sebnem; Kantarci, Gulten; Yurdasiper, Aysu; Ege, Mehmet AliThe aim of the study was to accelerate the dissolution of the sustained release dosage forms using both elevated temperature and high rpm rates. Teokap (R) SR 200 mg pellets were tested by in vitro sustained and accelerated dissolution studies using USP XXIII rotating paddle method. Sustained dissolution studies were carried out for 12 h in phosphate buffer at 37 +/- 0.5 degrees C and 50 rpm. Accelerated dissolution studies were performed for 48 min in distilled water at 90 +/- 1 degrees C and 250 rpm. The results obtained from accelerated and sustained dissolution studies were correlated using both linear and linear kinetic correlation methods by a computer program. While r(2) and maximum error between calculated and observed drug release rates were found to be 0.9129 and 15.9%, respectively, in linear correlation method, these values were observed as 0.9938 and 3.6%, respectively, in linear kinetic correlation method. In vivo plasma concentration data were obtained from six New Zealand rabbits after administration of a single dose of Teokap (R) SR 200 mg pellet. Then, the results of in vivo study were evaluated with in vitro accelerated and sustained dissolution results by applying them to in vitro-in vivo linear correlations. As a result of these correlations, it was shown that the in vitro correlation plots were very similar to the plot which was obtained by the in vivo study (f(2) = 73.81-77.11). This study suggested a way to prevent the loss of time for routine dissolution studies of sustained release preparations for quality control procedures using in vitro accelerated dissolution tests. The storage and quarantine periods of the product in process and process controls in the manufactories could be shortened by this method. Calculation of the in vivo performance of sustained release dosage forms using the results of the accelerated dissolution studies may be counted as another advantage of the method.Öğe Characterization and Antimicrobial Activity of Novel Hazelnut Oil Microemulsion Loaded with Mitomycin C(Colegio Farmaceuticos Provincia De Buenos Aires, 2015) Kotmakci, Mustafa; Ozturk, Ismail; Kantarci, Gulten; Ermertcan, SafakThe aim of this study was to prepare and characterize a mitomycin C-loaded hazelnut oil microemulsion, and to examine its antimicrobial activity on bacteria and fungi. Microemulsions were prepared by titration of the oil-surfactant mixture with the water phase, and characterized for their physicochemical properties. In vitro release study was carried out by dialysis technique. In vitro antimicrobial activity on bacteria and fungi was examined by microdilution method and disc diffusion test. The hazelnut oil microemulsion has a droplet size of 27.6 nm which increased to 52.0 nm after mitomycin C loading. Mitomycin C-hazelnut oil microemulsion showed more effective bactericidal activity on P. aeruginosa, B. subtilis and S. aureus as compared with mitomycin C-solution. In conclusion, our novel microemulsion may be a promising carrier system for mitomycin C to increase its therapeutic effect on the infections originated from P. aeruginosa, S. aureus and B. subtilis.Öğe Determination of In Vivo Behavior of Mitomycin C-Loaded O/W Soybean Oil Microemulsion and Mitomycin C Solution Via Gamma Camera Imaging(Mary Ann Liebert, Inc, 2013) Kotmakci, Mustafa; Kantarci, Gulten; Asikoglu, Makbule; Ozkilic, Hayal; Ertan, GokhanIn this study, a microemulsion system was evaluated for delivery of mitomycin C (MMC). To track the distribution of the formulated drug after intravenous administration, radiochemical labeling and gamma scintigraphy imaging were used. The aim was to evaluate a microemulsion system for intravenous delivery of MMC and to compare its in vivo behavior with that of the MMC solution. For microemulsion formulation, soybean oil was used as the oil phase. Lecithin and Tween 80 were surfactants and ethanol was the cosurfactant. To understand the whole body localization of MMC-loaded microemulsion, MMC was labeled with radioactive technetium and gamma scintigraphy was applied for visualization of drug distribution. Radioactivity in the bladder 30 minutes after injection of the MMC solution was observed, according to static gamma camera images. This shows that urinary excretion of the latter starts very soon. On the other hand, no radioactivity appeared in the urinary bladder during the 90 minutes following the administration of MMC-loaded microemulsion. The unabated radioactivity in the liver during the experiment shows that the localization of microemulsion formulation in the liver is stable. In the light of the foregoing, it is suggested that this microemulsion formulation may be an appropriate carrier system for anticancer agents by intravenous delivery in hepatic cancer chemotherapy.Öğe Determining in vitro anticancer activity of mitomycin C loaded microemulsion(Current Biology Ltd, 2011) Kotmakchiev, Mustafa; Cetintas, Vildan Bozok; Kantarci, GultenÖğe Development of novel cationic solid lipid nanoparticles as gene delivery system: Characterization and transfection ability(Current Biology Ltd, 2011) Akbaba, Hasan; Kotmakchiev, Mustafa; Dora, Devrim Demir; Korkmaz, Ceren; Kantarci, GultenÖğe Development of novel precirol based cationic solid lipid nanoparticles by microemulsion dilution method as DNA delivery system(Current Biology Ltd, 2011) Kotmakchiev, Mustafa; Akbaba, Hasan; Dora, Devrim Demir; Korkmaz, Ceren; Kantarci, GultenÖğe PREPARATION AND CHARACTERISATION OF CATIONIC SOLID LIPID NANOPARTICLES AS A POTENTIAL DNA DELIVERY SYSTEM(Elsevier Science Bv, 2009) Kantarci, Gulten; Dora, Devrim Demir; Akbaba, HasanÖğe PREPARATION AND CHARACTERISATION OF CATIONIC SOLID LIPID NANOPARTICLES AS A POTENTIAL DNA DELIVERY SYSTEM(Elsevier Science Bv, 2009) Kantarci, Gulten; Dora, Devrim Demir; Akbaba, HasanÖğe Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA(Univ Sao Paulo, Conjunto Quimicas, 2018) Karagoz, Ugur; Kotmakci, Mustafa; Akbaba, Hasan; Cetintas, Vildan Bozok; Kantarci, GultenIn recent years, non-viral delivery systems for plasmid DNA have become particularly important. They can overcome the disadvantages of viral systems such as insertional mutagenesis and unpredicted immunogenicity. Some additional advantages of non-viral gene delivery systems are; good stability, low cost, targetability, delivery of a high amount of genetic materials. The aim of the study was to develop novel non-viral nanosystems suitable for gene delivery. Two formulations were developed for this purpose: water-in-oil microemulsion (ME) and solid lipid nanoparticles (SLN). The microemulsion was composed of Peceol, Tween 80, Plurol oleique, ethanol and water. The SLN was consisting of Precirol, Esterquat-1 (EQ1), Tween 80, Lecithin, ethanol and water. Characterization studies were carried out by measuring particle size, zeta potential, viscosity and pH. TEM imaging was performed on SLN formulations. Protection against DNase I degradation was examined. Cytotoxicity and transfection efficacy of selected formulations were tested on L929 mouse fibroblast cells. Particle sizes of complexes were below 100 nm and with high positive zeta potential. TEM images revealed that SLNs are spherical. The SLN: DNA complexes have low toxicity and good transfection ability. All results showed that the developed SLN formulations can be considered as suitable non-viral gene delivery systems.Öğe PREPARATION OF W/O MCIROEMULSIONS WITH DIFFERENT VEGETABLE OILS FOR DRUG DELIVERY(Elsevier Science Bv, 2009) Kantarci, Gulten; Kotmakchiev, Mustafa; Aksu, Buket; Guneri, TamerÖğe PREPARATION OF W/O MCIROEMULSIONS WITH DIFFERENT VEGETABLE OILS FOR DRUG DELIVERY(Elsevier Science Bv, 2009) Kantarci, Gulten; Kotmakchiev, Mustafa; Aksu, Buket; Guneri, Tamer
