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Biocompatible Delivery System for Metformin: Characterization, Radiolabeling and in Vitro Studies
(Bentham Science Publ Ltd, 2020) Aydin, Burcu; Ucar, Eser; Tekin, Volkan; Ichedef, Cigdem; Teksoz, Serap
Background: in recent years, the uses of nanotechnology in medicine have an increasing potential as an effective nanocarrier system. These systems are improved with the purpose of maximizing therapeutic activity and minimizing undesirable side-effects. Moreover, radiolabeled nanoparticles can be used as agents for diagnosis and therapeutic purposes in clinical applications. They have three main components: the core, the targeting biomolecule, and the radionuclide. Objective: It is aimed to synthesize Metformin (MET) loaded Solid Lipid Nanoparticles (MET-SLN) and radiolabeled with technetium-99m tricarbonyl core. Methods: the structure of synthesized nanoparticles was characterized by Fourier Transform Infrared Spectroscopy (FTIR). the particle size and morphology of nanoparticles were examined by Dynamic Light Scattering (DLS), and Scanning Electron Microscope (SEM). Quality control studies of radiolabeled MET-SLN [Tc-99m(CO)(3)-MET-SLN] were performed by High-Performance Liquid Radiochromatography (HPLRC) and Thin Layer Radiochromatography (TLRC). Results: the radiolabeling yield of [Tc-99m(CO)(3)-MET-SLN] was found to be 88%. in vitro studies have been performed on cancer lines(MCF7, MDA-MD-231 breast, and HEPG2 liver cancer cells) to determine the biological behavior of Tc-99m(CO)3-MET-SLNs. Conclusion: the results showed that higher uptake values were observed on estrogen-positive MCF7 breast cancer cell line according to estrogen negative MDA-MB-231 breast cancer and HEPG2 liver cancer cell lines.
Biodistribution of radiolabeled alpha-amanitin in mice: An Investigation
(Pergamon-Elsevier Science Ltd, 2024) Durkan, Kubra; Ichedef, Cigdem; Baris, Elif; Arici, M. Aylin
Mushroom poisonings caused by Amanita phalloides are the leading cause of mushroom -related deaths worldwide. Alpha-Amanitin (alpha-AMA), a toxic substance present in these mushrooms, is responsible for the resulting hepatotoxicity and nephrotoxicity. The objective of our study was to determine the distribution of alpha-AMA in Balb/c mice by labeling with Iodine -131. Mice were injected with a toxic dose (1.4 mg/kg) of alpha-AMA labeled with Iodine -131. The mice were sacrificed at the 1st, 2nd, 4th, 8th, 24th, and 48th hours under anesthesia. The organs of the mice were removed, and their biodistribution was assessed in all experiments. The percent injected dose per gram (ID/g %) value for kidney, liver, lung, and heart tissues at 1st hour were 1.59 +/- 0.07, 1.25 +/- 0.33, 3.67 +/- 0.80 and 1.07 +/- 0.01 respectively. This study provides insights into the potential long-term effects of alpha-AMA accumulation in specific organs. Additionally, this study has generated essential data that can be used to demonstrate the impact of antidotes on the biological distribution of alpha-AMA in future toxicity models.
Bioevaluation of Tc-99m(I) carbonyl-radiolabeled amino acid coated magnetic nanoparticles in vivo
(Elsevier Science Sa, 2019) Buyukok, Onur; Ucar, Eser; Ichedef, Cigdem; Cetin, Oguz; Teksoz, Serap
In this paper, amino acid coated and radiolabeled magnetic nanoparticles (MNPs) were prepared as a potential diagnostic agent. Magnetic nanoparticles were synthesized and functionalized by L-alanine and L-cysteine amino acids in one step. Besides surface functionalization, amino acid coating also provided favourable particle size. Structural characterization was performed by scanning electron microscopy. We obtained 30-38 nm and 22-30 nm diameter ranges with spherical shape for L-alanine and L-cysteine coated nanoparticles, separately. Then L-alanine and L-cysteine coated MNPs were radiolabeled with Tc-99m(I)-tricarbonyl core Tc-99m(CO)(3)-Alanine-MNP, Tc-99m(CO)(3)-Cysteine-MNP) with a labeling yield of 63 +/- 3% and 74 +/- 2%. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of Tc-99m (CO)(3)-Alanine-MNP and Tc-99m(CO)(3)-Cysteine-MNP were found as 94.3 +/- 0.47% and 97.6 +/- 1.06% in each of the final solution, respectively. in addition, both radiolabeled nanoparticles were stabile up to 4 h at 37 degrees C temperature in vitro conditions.
Complexation of gallic acid involving La3+, Sm3+, Th4+ and UO22+ ions in aqueous solution by potentiometry at various temperatures
(Springer, 2024) Sismanoglu, Tuba; Ichedef, Cigdem; Akdut, Gizem; Soylu, Guelin Selda Pozan; Medine, Emin Ilker; Teksoz, Serap
In this study, the complexation of gallic acid with various lanthanide and actinide ions (La3+, Sm3+, Th4+, and UO22+) was investigated under aqueous conditions at elevated temperatures by potentiometric titration, with ionic strength of I = 0.10 mol dm-3 KCl. Stability constants for complexation of related metal-ions were determined using HYPERQUAD2013 software. The formation of complexes was considered at temperatures of 25 degrees C, 37 degrees C, and 45 degrees C. By examining the dependence of complex formation on temperature, the thermodynamic parameters of each complex were calculated. It was observed that the stability constant values varied with temperature. Additionally, cytotoxicity studies were conducted on MCF-7 and A-549 cell lines as part of the conclusion.
Design of docetaxel-loaded polymeric nanoparticles: characterization, radiolabeling with Tc-99m and in vitro evaluation
(Springer, 2020) Cetin, Oguz; Ichedef, Cigdem; Aydin, Burcu; Teksoz, Serap
This research aims to develop a polymeric drug delivery system for docetaxel (DTX) and to investigate its radiotracer potential by in vitro. DTX loaded Poly(lactic acid)/Poly(ethylene glycol) diblock copolymer (PLA-PEG) was synthesized and radiolabeled with technetium-99m tricarbonyl core. PLA-PEG-DTX nanoparticles had a particle size and zeta potential 120 +/- 7.3 nm and - 24.66 +/- 1.5 mV respectively, which was further supported by SEM image. the radiolabeling efficiency of Tc-99m(CO)(3)-PLA-PEG-DTX was found to be 90 +/- 3.51%,. in vitro study was carried out on cancer cell lines (MCF7, MDA-MB-231) to examine the biological behavior of docetaxel loaded PLA-PEG nanoparticles. [GRAPHICS] .
Design of Tc-99(m) radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation
(Elsevier Science Sa, 2021) Ichedef, Cigdem; Teksoz, Serap; Cetin, Oguz; Aydin, Burcu; Sarikavak, Ibrahim; Parlak, Yasemin; Bilgin, Bedriye Elvan
The main objective of this study is to create a multifunctional drug carrier system as a prototype product. For this purpose, Gemcitabine that used as an anticancer agent for several kinds of tumour therapy, and technetium(I)-tricarbonyl core, [Tc-99m(CO)(3)](+), was incorporated to the polymeric structure. in the current study PLA-PEG-COOH (polylactic acid-polyethylene glycol-carboxyl) copolymer was synthesized and the structure analysis was performed by FT-IR and H-1 NMR. The surface charge, size and morphology of the polymeric nanoparticles was evaluated by Zeta Potential (ZP) analysis, Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM) methods. Gemcitabine loaded polymeric nanoparticles were radiolabeled with [Tc-99m(CO)(3)(H2O)(3)](+) and quality controls were performed by thin layer radiochromatograpy (TLRC) and high performance liquid radiochmmatograpy (HPLRC). PLA-PEG-GEM complex was labeled with [Tc-99m(CO)(3)](+) in high radiochemical yield and purity. The radiolabeled complex is stable during the study period in saline solution. The biological activity of this radiolabeled system was evaluated performing biodistribution and gamma camera imaging on Wistar Albino rats which exhibited higher uptake in the lung and liver at all selected time points.
Effect of a plant origin drug on the biodistribution of Tc-99m-DTPA in Wistar albino rats
(Soc Brasileira Farmacognosia, 2012) Zora, Hasan; Muftuler, Zumrut F. Biber; Demir, Ilknur; Kilcar, Ayfer Yurt; Ichedef, Cigdem; Unak, Perihan
In recent years all over the world, medicinal plants are used quite a lot but side effects of biological and chemical contents and radiopharmaceutical interactions for each consumer in question aren't entirely well-known. The studies of plant origin drug interaction with radiopharmaceuticals are highly relevant and desired. One of them is passiflora syrup (Passiflora incarnata L., Passifloraceae) which is widely used for depression, insomnia, anxiety and menopause period. The aim of current study is to evaluate possible effects of passiflora syrup on the biodistribution of Tc-99m-DTPA and its blood cells uptake. DTPA was labeled with Tc-99m radionuclide. Biodistribution studies were performed on male Wistar albino rats which were treated via oral feeding-gavage-method with either passiflora syrup or 0.9 % NaCl as control group for ten days. Blood samples were obtained by cardiac blood withdrawal from the rats and they were radiolabeled. The biodistribution results showed that the passiflora syrup decreased the uptake of Tc-99m-DTPA in kidneys and in blood cells. Tc-99m-DTPA being used widely as a kidney diagnostic agent in nuclear medicine seems to be interacting with orally taken passiflora. Passiflora syrup may modify the uptake of Tc-99m-DTPA by kidney. The knowledge of this negative effect may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in nuclear medicine.
Effect of rosemary (Rosmarinus officinalis) extract on the biodistribution of Tc-99m sulphur colloid and on the radiolabeled blood constituents
(Soc Brasileira Farmacognosia, 2013) Ucar, Eser; Teksoz, Serap; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Unak, Perihan
With this study we evaluated the effects of the herb rosemary (Rosmarinus officinalis L. Lamiaceae) extract on the labeling of blood constituents with technetium-99m (Tc-99m) labeled sulphur colloid and on the biodistribution of Tc-99m-Sulphur Colloid in Wistar albino rats. For this purpose, two groups of animals (male wistar rats, 130140 g) were treated (1 mL) with a rosemary extract (750 mg/kg body wt., n=9) and water (control, n=9) separately by gavage for five days. Tc-99m-Sulphur Colloid was administrated by intravenous injection; organs/tissues were withdrawn and weighted. Blood was centrifuged, plasma and blood cells were isolated. The radioactivity was counted to calculate the percentage of activity per gram for each organ/tissue and percentage of activity in blood cells and plasma. A significant increase (p<0.05) in the uptake of Tc-99m-Sulphur Colloid in the liver after the treatment with rosemary extract was observed. These results indicate that the substances or metabolites of the rosemary extract would change the biodistribution of Tc-99m-Sulphur Colloid.
Effects of broccoli extract on biodistribution and labeling blood components with Tc-99m-GH
(Acta Cirurgica Brasileira, 2011) Cekic, Betul; Muftuler, Fazilet Zumrut Biber; Kilcar, Ayfer Yurt; Ichedef, Cigdem; Unak, Perihan
Purpose: People consume vegetables without the knowledge of the side effects of the biological and chemical contents and interactions between radiopharmaceuticals and herbal extract. To this end, current study is focused on the effects of broccoli extract on biodistribution of radiolabeled glucoheptonate (Tc-99m-GH) and radiolabeling of blood components. Methods: GH was labeled with Tc-99m. Quality control studies were done utilizing TLC method. Biodistribution studies were performed on male rats which were treated via gavage with either broccoli extract or SF as control group for 15 days. Blood samples were withdrawn from rats' heart. Radiolabeling of blood constituents performed incubating with GH, SnCl2 and Tc-99m. Results: Radiochemical yield of Tc-99m-GH is 98.46 +/- 1.48 % (n=8). Biodistribution studies have shown that according to the control, the treated group with broccoli has approximately 10 times less uptake in kidney. The percentage of the radioactivity ratios of the blood components is found to be same in both groups. Conclusions: Although there is no considerable effect on the radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially on kidneys. The knowledge of this change on kidney uptake may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in Nuclear Medicine.
Hydrolytic Behavior of La3+ and Sm3+ at Various Temperatures
(Springer/Plenum Publishers, 2018) Ichedef, Cigdem; Sismanoglu, Tuba; Teksoz, Serap
The hydrolytic species of lanthanide ions, La3+ and Sm3+, in water at I = 0.1 mol center dot dm(-3) KCl ionic strength and temperatures of 298.15, 310.15 and 318.15 K were investigated by potentiometry. The hydrolytic species were modeled by the HySS simulation program. From the results, the hydrolytic species of each metal ion at different temperatures were calculated using the program HYPERQUAD2013. The hydrolysis constants (log(10) beta) of [La(OH)](2+) and La(OH)(3) were calculated as -8.52 +/- 0.46, -26.84 +/- 0.48, and log(10) beta values of [Sm(OH)](2+), [Sm(OH)(2)](+), Sm(OH)(3) were calculated as -7.11 +/- 0.21, -15.84 +/- 0.25 and -23.44 +/- 0.52 in aqueous media at 298.15 K, respectively. The dependence of the hydrolysis constants on the temperature allowed us to calculate the enthalpy, entropy, and Gibbs energy of hydrolysis values of each species.
In vitro evaluation of radiolabeled methotrexate loaded magnetic nanoparticle delivery system
(Springer, 2023) Uzug, Sara; Cetin, Oguz; Aydin, Burcu; Ichedef, Cigdem; Teksoz, Serap
Magnetic nanoparticles were synthesized via the chemical co-precipitation method using ammonium hydroxide as a precipitating agent. For this purpose, iron magnetic nanoparticles (Fe3O4) were synthesized, coated with alanine (Ala) and loaded with a folic acid antagonist MTX. Structural characterization of MNP-Ala and MNP-Ala-MTX were achieved by nanoparticle characterization techniques. MTX loaded magnetic nanoparticle was radiolabeled with technetium-99 m with 89% efficiency.The effect of radiolabeled nanoparticles on cells was determined on human cervical cancer (HeLa) and human breast (MCF7) cancer cell lines. It was found that drug loaded MNP nanoparticles showed higher uptake in MCF7 cells than in HeLa cells.
In Vitro/In Vivo Evaluation of Radiolabeled [Tc-99m(CO)(3)](+)-Hydroxyurea and Fluorescein Isothiocyanate-Hydroxyurea
(Mary Ann Liebert, Inc, 2016) Yilmaz, Baris; Teksoz, Serap; Kilcar, Ayfer Yurt; Ucar, Eser; Ichedef, Cigdem; Medine, Emin Ilker; Ari, Kadir
The aim of current study is to examine hydroxyurea (HU), which is an antineoplastic drug used for the treatment of leukemia, sickle-cell disease, HIV, psoriasis, thrombocythemia, and various neoplastic diseases in two aspects. The active ingredient hydroxyurea was obtained by purification of the capsule form drug, commercially named as HYDREA. Then, [Tc-99m(CO)(3)](+)core radiolabeling with HU was performed as first aspect. Quality control studies of Tc-99m(CO)(3)-HU complex were performed by thin-layer radiochromatography and high-performance liquid radiochromatography methods. The results demonstrated that the radiolabeling yield was quite high (98.43%+/- 2.29%). Also, Tc-99m(CO)(3)-HU complex has good stability during the 24-hour period. Biological behavior of Tc-99m(CO)(3)-HU complex is evaluated by biodistribution studies on Wistar Albino rats. Fluorescein isothiocyanate (FITC) labeling of HU was performed as second aspect. Fluorometric evaluation of binding efficacy and fluorescence imaging studies on MCF7 and Hela cell lines were carried out. It was thought that the knowledge achieved in this study would contribute to using Tc-99m(CO)(3)-HU complex as an imaging agent, which inhibits the DNA synthesis selectively, by inhibiting ribonucleotide reductase enzyme. It was observed that FITC-HU has noteworthy incorporation on both cell lines.
Leucine Complexes with Cerium: Stability Constants and Thermodynamic Parameters
(Amer Chemical Soc, 2011) Sismanoglu, Tuba; Teksoz, Serap; Ichedef, Cigdem
The complexation of leucine (Leu) with Ce3+ was investigated using the potentiometric method at different ionic strengths (I = (0.10 and 1.00) mol dm(-3)) in KCl medium at elevated temperatures. The stability constants of the leucine complexes with Ce3+ at metal:ligand (M:L) molar ratios of 1:1, 1:3, and 1:5 were determined. From the data obtained, stability constants were calculated using the program HYPERQUAD2006 for proton-ligand and metal-ligand species. The formation of monomeric species was taken into consideration for Ce3+ at (25, 37, and 45)degrees C. Thermodynamic parameters were calculated for all possible leucine complexes. In conclusion, it is indicated that the formation of complexes change the value of the stability constants at various ionic strengths and temperatures.
Magnetic nanoparticle-conjugated and radioiodinated-DESG: in vitro and in vivo efficiency investigation
(Springer, 2015) Yilmaz, Tayfun; Unak, Perihan; Muftuler, F. Zumrut Biber; Medine, E. Ilker; Ichedef, Cigdem; Kilcar, Ayfer Yurt
The purpose of this work is to combine magnetite nanoparticles with radioiodinated diethylstilbestrol-glucuronide (DESG) to get a prostate cancer agent. Magnetically targeted drug delivery by particulate carriers is an efficient method of delivering drugs to localized and targeted disease sites, such as tumors. Estrogen glucuronide derivative; DESG was synthesized which is specific for beta glucuronidase enzyme consisting tumor cells and conjugated with iron oxide nanoparticles (NP) and radioiodinated with I-125/131 to evaluate in vitro/in vivo radiopharmaceutical potential of NP conjugated DESG (NP-DESG) and magnetic field applied NP-DESG (MNP-DESG). According to cell culture studies, incorporation ratios of MNP-DESG were higher in MCF7 cells than A549 and Caco2 cells. Biodistribution experiments were verified that the range of the breast/blood and breast/muscle ratios was approximately between 1.82 and 10.10 in 240 min for ER unsaturated studies. The results are promising for targeted therapy of both estrogen receptor and enzyme beta-glucuronidase rich cancers.
A new approach to liquid penetrant inspection: radiolabeled QDots
(Springer, 2017) Sezgin, Hasan Sezer; Muftuler, Fazilet Zumrut Biber; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Teksoz, Serap; Unak, Perihan; Harmansah, Coskun
Penetrant technique is a sensitive non-destructive testing (NDT) method for detecting and locating the presence of cracks in sample surface. Today, NDT is used in a wide range of industries including aerospace, biotechnology, defence, marine, oil-gas and energy plants. This work focuses on potential use of radiolabeled ZnS coated CdTe quantum dots (QDots) as a penetrant for liquid penetrant testing. The synthesized QDots as a precursor were tested for surface defects detection in welded joints. The experimental results show the highest activities were found in defects on the sample surface. These finding are consistent with the sample NDT inspection test report.
Polymer Coated Iron Nanoparticles: Radiolabeling & In vitro Studies
(Bentham Science Publ Ltd, 2021) Yilmaz, Selin; Ichedef, Cigdem; Karatay, Kadriye Busra; Teksoz, Serap
Background: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it has been aimed to develop a novel targeted Tc-99m radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with Tc-99m. SPIONs were synthesized by reduction-coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM), respectively. The radiolabeling yield of SPION-PLGAGEM nanoparticles was determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles was deter- mined as 366.6 nm by DLS, while zeta potential was found as 29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4 +/- 0.16% by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles was determined as 97.8 +/- 1.75% via TLRC. Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, while the incorporation rate was increased for both cell lines with external magnetic field application. Conclusion: Tc-99m labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and super-paramagnetic characteristics
Preparation and bioevaluation of Tc-99m-carbonyl complex of guanine
(Springer, 2011) Ichedef, Cigdem; Teksoz, Serap; Senocak, Kamile; Ucar, Eser; Kilcar, Ayfer Yurt
The aim of this study is to prepare radiolabeled guanine with Tc-99m(CO) (3) (+) core. For this purpose, guanine has been radiolabeled with Tc-99m(CO) (3) (+) core. Quality control study of radiolabeled guanine molecule with Tc-99m(CO) (3) (+) core was performed by thin layer radio chromatography (TLRC) and high performance liquid radio chromatography (HPLRC). The results showed that the radiolabeling yield was quite high (94 +/- A 3%). Beside that Tc-99m(CO)(3)-Gua complex has showed good in vitro stability during the 24 h period. Radiopharmaceutical potential of this complex was evaluated in Wistar Albino Rats. It was concluded that Tc-99m(CO)(3)-Gua could be used as a nucleotide radiopharmaceutical for in vivo applications.
Preparation of technetium-99m labeled ibuprofen by direct route and technetium-99m tricarbonyl route: a comparison ofin vivobehaviors
(Taylor & Francis Ltd, 2020) Durkan, Kubra; Ichedef, Cigdem; Yurt Kilcar, Ayfer; Arici, Mesut; Ucar, Eser
In the current study, ibuprofen (ibu) which is a non-steroidal anti-inflammatory drug (NSAID) was radiolabeled with(99m)Tc using two different methods: stannous chloride method (direct route) and technetium-99m tricarbonyl [Tc-99m(CO)(3)](+)route. Thus, it's aimed to investigate the radiolabeling potential of ibu for inflammation detection and to monitor if there is any difference inin vivodistribution depending on the radiolabeling route. Quality control studies of both radiolabeled ibu were performed by radiochromatographic methods (Thin Layer Liquid Radio Chromatography and High Performance Liquid Radio Chromatography). Radiolabeling yields of(99m)Tc-ibu and(99m)Tc(CO)(3)-ibu were determined as 99.05 +/- 0.83% and 91.79 +/- 3.30% (n = 5), respectively. Experimental lipophilicities of both radiolabeled ibu were determined. the biological behavior of both radiolabeled ibu was investigated in healthy Albino Wistar male rats byin vivobiodistribution studies. It was seen that both radiolabeled ibuprofen showed renal excretion while organ uptakes of(99m)Tc-ibu and(99m)Tc(CO)(3)-ibu differ against time.
A promising radiolabeled drug delivery system for methotrexate: synthesis and in vitro evaluation of Tc-99m labeled drug loaded uniform mesoporous silica nanoparticles
(Springer, 2021) Ichedef, Cigdem; Aydin, Burcu; Hamurisci, Selin Irdel; Teksoz, Serap; Medine, Emin Ilker
In present study, we describe a promising radiolabeled drug delivery system for Methotrexate (MTX), an anticancer drug used in the treatment of breast cancer. Uniform and re-dispersible MSN were synthesized with a particle size of as 42.55 +/- 1.45 nm. Then, MTX was loaded into the surface modified MSN with DTPA over 95% loading capacity. Subsequently, MTX loaded MSN carrier system was radiolabeled with Tc-99 m (Tc-99m-MTX-MSN) with 92.20 +/- 0.8% radiolabeling yield. Furthermore, in vitro evaluation on estrogen positive (ER +) MCF7 and estrogen negative (ER-) A549 cells lines were performed for determining apoptotic and cytotoxic effects of MTX-MSN, and incorporation behavior of Tc-99m-MTX-MSN. Drug loaded MSN particles were exhibit higher uptake in MCF7 cells than A549 cells.
Radioiodine labeled CdSe/CdS quantum dots: Lectin targeted dual probes
(Walter De Gruyter Gmbh, 2014) Akca, Ozlet; Unak, Perihan; Medine, E. Ilker; Sakarya, Serhan; Kilcar, Ayfer Yurt; Ichedef, Cigdem; Bekis, Recep; Timur, Suna
CdSe/CdS quantum dots (QD) were synthesized and bioconjugated with Sambucus nigra agglutinin (SNA) lectin (Lec). Mannose triflate and cysteamine molecules (MTC) were also utilized to prepare MTC-QDs and MTC-QDs-Lec probes as well as Lec bound QDs. Afterwards; potential use of these nanoparticles as radiolabeled fluorescence nano-probes for the cell imaging studies has been investigated. Biological activities of I-125(-), I-125-MTC-QDs, MTC-QDs- Lec-I-125, QDs-Lec-I-125 and Lec-I-125 were examined on various cancer cell lines such as Caco-2, MCF-7 and A-549 in terms of cell incorporation. QDs-Lec-I-125 exhibited the highest cell incorporation on whole cell lines. In addition, the QDs-Lec-I-131, was used for in vivo imaging. The whole body distribution of the radiolabeled QDs on New Zealand rabbits and Balb C mice were examined by taking dynamic and static images. Radioactivity cleared from the kidneys and the bladder, while significant amount radioactivity was retained in the heart and liver within 24 h.

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