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Öğe Astragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.(National Institute of Science Communication and Information Resources (NISCAIR), 2015) Nartop P.; Gürel A.; Akgün I.H.; Bedir E.In the present paper, an efficient method for clonal propagation of an endemic plant, Astragalus trojanus Stev., was described. Effect of sucrose and 6-benzyladenine (BA) concentrations on shoot proliferation and rooting was studied. Woody plant medium (WPM) supplemented with two different concentrations of BA (S1 medium with 0.5 mg/L BA&S2 medium with 1 mg/L BA) was used, where S2 medium resulted in higher rates in both shoot elongation and multiple shoot proliferation of clonally propagated A. trojanus shoot cultures. Of 27 clones, 3 were selected for further studies based on the highest regeneration capacity (Clones 1, 12&15). A total of 340 shoots were obtained from Clone 1 in S1 medium. In this medium, the rooting ratio of shoots for Clone 15 was 11.67%. The highest shoot elongation (1.99 cm), multiple shoot proliferation ratio (4.35) and multiple shoot proliferation percentage (91.67%) were obtained from Clone 1 in S2 medium. Two media, WPM medium supplemented with 15 g/L (K1 medium) and 30 g/L (K2 medium) sucrose were used for rooting. The root formation of Clone 1 was 26.67% in K2 medium. The highest astragaloside IV (0.5486 µg/mg) and cycloastragenol (0.1263 µg/mg) contents were detected in roots grown in K1 medium.Öğe The characteristics attributes and performance of naturally colored cotton(2003) Öktem T.; Gürel A.; Akdemir H.Colored cotton fibers have become increasingly popular for the production of ecologically beneficial textile products. Their use avoids the wet process treatment required for white cotton, and eliminates the production costs of dye consumption. The outcome of naturally colored fiber usage using light brown, dark brown, and green cotton fibers has been evaluated. It was found that naturally colored cotton fibers have sufficient levels of colorfastness.Öğe Correction to: High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA® temporary immersion bioreactor in Cattleya forbesii Lindl. (Plant Cell, Tissue and Organ Culture (PCTOC), (2019), 136, 3, (451-464), 10.1007/s11240-018-1526-2)(Springer Netherlands, 2019) Ekmekçigil M.; Bayraktar M.; Akkuş Ö.; Gürel A.In the original article, part a in Fig. 2 was incorrect. The correct Fig. 2 is printed below, and the first author’s current affiliation has been added. Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2019, Springer Nature B.V.Öğe Ecological materials and methods in the textile industry: Atmospheric-plasma treatments of naturally colored cotton(2011) Demir A.; Özdogan E.; Özdil N.; Gürel A.Naturally colored cotton, in accordance with currently increasing interest in ecological textile products and methods, has increased in popularity. Commerce is another of the primary reasons along with interest in environmentally friendly and niche-concept approaches. However, the color palette is limited; no bleaching or dyeing process is used. Instead, only a pretreatment to make the fibers hydrophilic is necessary. This can be induced with several different methods. In this respect, atmospheric-plasma treatments have emerged as an alternative. In this study, knitted and naturally colored cotton fabrics were treated with argon and air atmospheric plasma. The hydrophilicity, wickability, surface friction coefficient, air permeability, water vapor permeability, thermal conductivity, thermal resistance, and fastness were investigated. The surfaces of untreated and plasma-treated fabrics were analyzed with Fourier transform infrared/attenuated total reflectance and scanning electron microscopy to detect and compare the chemical and morphological modifications. The results revealed that atmospheric-plasma treatments are capable of modifying the surface of naturally colored cotton fabrics without any important loss in the color strength or fastness and thermal properties. © 2010 Wiley Periodicals, Inc.Öğe Erratum to Immobilization of Rubia tinctorum L. suspension cultures and its effects on alizarin and purpurin accumulation and biomass production (Plant Cell Tiss Organ Cult, 10.1007/s11240-012-0212-z)(2013) Nartop P.; Akay Ş.; Gürel A.[No abstract available]Öğe High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA ® temporary immersion bioreactor in Cattleya forbesii Lindl.(Springer Netherlands, 2019) Ekmekçigil M.; Bayraktar M.; Akkuş Ö.; Gürel A.An efficient in vitro mass propagation through protocorm-like bodies (PLBs) was established in Cattleya forbesii Lindl., a commercially important orchid. Whole PLBs (W-PLB) and transverse thin cell layers of PLB (tTCL-PLB) explants were cultured in RITA ® bioreactors based on temporary immersion system. Explants were transferred in semi-solid or RITA ® bioreactor for protocorm production or shoot regeneration. The effect of different immersion frequencies, medium volumes and inoculum densities were studied and optimized. RITA ® bioreactor cultures were found to be superior compared with semi-solid cultures regarding PLB production and shoot regeneration. tTCL-PLB explant types cultured in the RITA ® bioreactor with immersion for 1 min/4 h, 250 mL of medium and 20 explants showed the highest number of PLBs per RITA ® (2237 PLBs) and per explant (111.9 PLBs). The highest number of PLBs per explant was 21 times higher than those from semi-solid culture. The highest number of shoots per RITA ® (3998 shoots) and per explant (199.9 shoots) were observed on tTCL-PLB cultured in RITA ® bioreactor (1 min/4 h; 150 mL of medium and 20 explants). The highest number of shoots per explant was 95 times higher than those grown on semi-solid culture. Mass propagation of PLBs and shoots of C. forbesii Lindl. using combined thin cell layer and RITA ® temporary immersion has been adapted in commercial practice. © 2018, Springer Nature B.V.Öğe In vitro regeneration of vanilla (Vanilla planifolia L.)(Nobel Science and Research Center, 2017) Halim R.; Akyol B.; Gürel A.Vanilla is a high demanding product sold at high prices in world markets. Natural vanilla has high economic value and is theworld's second most expensive spices after saffron. Despite its economic value, it has been one of the least studied species of vanilla plants among other orchid species. Vanilla's synthetic form (vanillin) can be obtained from various chemical methods by using starting materials (coniferin, guaiacol, eugenol and lignin), but the vanilla flavor can naturally be obtained only from vanilla fruit. Micropropagation is an alternative technique for economically important plant production more effectively used with minimum starting material. For this reason, in the study, regeneration of the vanilla plant in vitro were carried out. Seed, young leaves, node and aerial parts of V. planifolia L. were used as an initial material. The highest callus regeneration (80%) from node explants was obtained in VK4 (MS+1 mg/L 2,4-D + 1 mg/L BAP) nutrient medium, while 100% direct shoot regeneration was provided in VK3 (MS+1 mg/L 2,4-D + 0,5 mg/L BAP) and VK6 (MS+1 mg/L BAP + 0,5 mg/L NAA) nutrient media. In addition, 50% rooting percentage was obtained in VK6 medium. Indirect shoot regeneration ratio was determined as 33,3% in VK2 (MS + 1 mg / L 2,4-D + 0,5 mg / L KIN) and 71,4% of indirect embryo formation was detected in VK4 medium. All embryos stayed in globules stages.Öğe Shoot Regeneration from Anther Culture of Sunflower (Helianthus annuus) and Some Interspecific Hybrids as Affected by Genotype and Culture Procedure(1991) Gürel A.; Nichterlein K.; Friedt W.Anther culture has been demonstrated to be an applicable technique for the development of doubled haploid, i. e. homozygous lines of many crop species. In some species, androgenetic doubled haploids have already been shown to be a useful tool for breeding. However, anther culture results in sunflower have been rather unsatisfactory up to now. As in other species, anther culture response of sunflower (Helianthus sp.) is strongly affected by physical, nutritional, physiological and genetical factors. By testing a number of different culture parameters, i. e. donor plant stages, culture media and conditions, and appropriate schedule could be worked out for the successful regeneration of shoots – at least for a number of sunflower lines and interspecific hybrids. Copyright © 1991, Wiley Blackwell. All rights reserved
