High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA ® temporary immersion bioreactor in Cattleya forbesii Lindl.
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Netherlands
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An efficient in vitro mass propagation through protocorm-like bodies (PLBs) was established in Cattleya forbesii Lindl., a commercially important orchid. Whole PLBs (W-PLB) and transverse thin cell layers of PLB (tTCL-PLB) explants were cultured in RITA ® bioreactors based on temporary immersion system. Explants were transferred in semi-solid or RITA ® bioreactor for protocorm production or shoot regeneration. The effect of different immersion frequencies, medium volumes and inoculum densities were studied and optimized. RITA ® bioreactor cultures were found to be superior compared with semi-solid cultures regarding PLB production and shoot regeneration. tTCL-PLB explant types cultured in the RITA ® bioreactor with immersion for 1 min/4 h, 250 mL of medium and 20 explants showed the highest number of PLBs per RITA ® (2237 PLBs) and per explant (111.9 PLBs). The highest number of PLBs per explant was 21 times higher than those from semi-solid culture. The highest number of shoots per RITA ® (3998 shoots) and per explant (199.9 shoots) were observed on tTCL-PLB cultured in RITA ® bioreactor (1 min/4 h; 150 mL of medium and 20 explants). The highest number of shoots per explant was 95 times higher than those grown on semi-solid culture. Mass propagation of PLBs and shoots of C. forbesii Lindl. using combined thin cell layer and RITA ® temporary immersion has been adapted in commercial practice. © 2018, Springer Nature B.V.
Açıklama
Anahtar Kelimeler
Bioreactor, Cattleya forbesii Lindl, In vitro, Mass propagation, Protocorm, Temporary immersion system, Thin cell layer
Kaynak
Plant Cell, Tissue and Organ Culture
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
136
Sayı
3
