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Öğe Antimicrobial activity of endemic Ziziphora taurica subsp. cleonioides (Boiss) P. H. Davis essential oil(University of Istanbul, 2006) Elgin G.; Yavaşoglu N.Ü.K.; Öztürk B.The aim of the present study is to investigate the antimicrobial activity of the essential oil prepared from aerial parts of the Turkish endemic Ziziphora taurica subsp. cleonioides (Boiss) P. H. Davis (Lamiaceae). The dried-in-shade at ambient temperature aerial parts of the plant were hydro-distilled for 3 h, using a modified Clevenger-type apparatus. In vitro antibacterial studies were carried out against eight bacteria strains and Candida albicans. The antimicrobial activity of the essential oil was carried out with the disc diffusion method. The results indicate that the essential oil remarkably inhibited the growth of tested microorganisms except Candida albicans.Öğe Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®(2012) Ince I.; Kayalar H.; Elgin G.; Koksal C.; Yavasoglu N.U.K.Context: Polyherbal formulations containing different plants are used for the treatment of various diseases. Romix® powder is a polyherbal formulation consisting of 14 traditionally used herbs and is used as a food supplement. There is no information about pharmaceutical activities of Romix®. Objective: This study determined the total phenolic and total flavonoid content, and investigated the antioxidant and anti-inflammatory activities and acute toxicity of Romix®. Material and methods: The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteu reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The quercetin content of the extract was analyzed using the high-performance liquid chromatography (HPLC) method. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the rat. Results: The flavonoid and phenolics contents of Romix® were 50.58 and 265.83mg/g in ethanol extract and 18.60 and 222.50mg/g in water extract, respectively. Total quercetin content of Romix® was determined as 2.857mg/g. Antioxidant activity results showed that ethanol extract in 1mg/mL concentration (4.49775 µg/mL) had moderate antioxidant activity than water extract in the same concentration (4.28191 µg/mL). Intraperitoneal administration of 25mg/kg Romix® extract exhibited anti-inflammatory activity and inhibited paw swelling at 1, 2, 3, 4, 5 and 6h in rats with no acute toxicity. Conclusion: These findings suggest that Romix® due to its antioxidant and anti-inflammatory activities may be useful in the prevention or treatment of aging-related and inflammatory diseases. © 2012 Informa Healthcare USA, Inc.Öğe Development and validation of a multidetector HPLC method for the determination of antioxidant flavonoids of some Hypericum L. species(2009) Elgin G.; Konyalioglu S.; Kilinc E.A multidetector approach for the HPLC analysis of methanolic extracts of three Hypericum species is performed under isocratic conditions through a C18 (ODS) analytical column. Variable wavelength UV-VIS detector (?max: 230 nm), fluorescence detector (ex: 250 nm em: 450 nm), and a programmable electrochemical detector (+1.30 V vs. Ag/AgCl) were employed simultaneously for detection. The mobile phase used was a combination of methanol and 0.01 M orthophosphoric acid (pH 7) (50:50, v/v). Retention time values were assigned relatively to those of the standards, and the flavonoid contents were quantified by the standard addition method. Rutin, quercetin-3'-glucoside (isoquercitrin), luteolin-4'-glucoside, quercetin-4'-glucoside, quercetin, naringenin, luteolin, and apigenin were chosen as the model compounds to undergo the validation studies with the three different detector systems. For the comparison of the detector performances, analytical method validation parameters were studied and displayed. Defined methods yielded pretty good results regarding linearity, precision (reproducibility), accuracy, limit of detection, and quantification. Copyright © Taylor & Francis Group, LLC.Öğe Effects of Pinus brutia bark extract and Pycnogenol® in a rat model of carrageenan induced inflammation(2009) Ince I.; Yesil-Celiktas O.; Karabay-Yavasoglu N.U.; Elgin G.The present study was conducted to explore the anti-inflammatory activities of Pinus brutia bark extract and Pycnogenol® in a rat model of carrageenan-induced inflammation. Firstly, the compositions of both samples were determined using HPLC. Then, carrageenan-induced paw edema was used to assess anti-inflammatory activity in mice. Paw volume was measured before and 1, 2, 3, 4, 5 and 6 h after the injection of carrageenan. Intraperitoneal administration of both the extract and Pycnogenol® inhibited paw swelling dose-dependently at 2, 3, 4, 5 and 6 h after carrageenan injection. Both samples exhibited significant anti-inflammatory activities at doses of 75 and 100 mg/kg body wt. between 2 and 4 hours after administration (p<0.05), respectively. Additionally, P. brutia bark extract showed significantly better activity at doses of 75 and 100 mg/kg body wt. than indomethacine at the dose of 10 mg/kg body wt. (p<0.05). No acute toxicity was identified in intraplantar injection of the extract at a dose of 2000 mg/kg body wt.. Therefore, P. brutia bark extract possessing 3.3-fold more total catechins and 9.8-fold more taxifolin than Pycnogenol® can be utilized as an anti-inflammatory agent. © 2009 Elsevier GmbH. All rights reserved.
