Development and validation of a multidetector HPLC method for the determination of antioxidant flavonoids of some Hypericum L. species
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A multidetector approach for the HPLC analysis of methanolic extracts of three Hypericum species is performed under isocratic conditions through a C18 (ODS) analytical column. Variable wavelength UV-VIS detector (?max: 230 nm), fluorescence detector (ex: 250 nm em: 450 nm), and a programmable electrochemical detector (+1.30 V vs. Ag/AgCl) were employed simultaneously for detection. The mobile phase used was a combination of methanol and 0.01 M orthophosphoric acid (pH 7) (50:50, v/v). Retention time values were assigned relatively to those of the standards, and the flavonoid contents were quantified by the standard addition method. Rutin, quercetin-3'-glucoside (isoquercitrin), luteolin-4'-glucoside, quercetin-4'-glucoside, quercetin, naringenin, luteolin, and apigenin were chosen as the model compounds to undergo the validation studies with the three different detector systems. For the comparison of the detector performances, analytical method validation parameters were studied and displayed. Defined methods yielded pretty good results regarding linearity, precision (reproducibility), accuracy, limit of detection, and quantification. Copyright © Taylor & Francis Group, LLC.
Açıklama
Anahtar Kelimeler
ECD, Flavonoids, FLD, HPLC analysis, Hypericum, UVD
Kaynak
Journal of Liquid Chromatography and Related Technologies
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
32
Sayı
3
