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Öğe The 2020 İzmir earthquake and its effect on COVID-19 cases(CSIRO, 2023) Durmaz, S.; Aslan, G.; Durusoy, R.; Çiçek, C.On 30 October 2020, an earthquake measuring 6.9 moment magnitude occurred between Kusadasi and Samos, Türkiye, with a significant impact on buildings in the Bornova–Bayraklı district of İzmir. Immediately afterwards, a comprehensive study was commenced to investigate the relationship between the earthquake that occurred during the SARS-CoV-2 (COVID-19) pandemic and case numbers at the Ege University, Faculty of Medicine’s Hospital in Bornova, İzmir. This cross-sectional study was carried out based on the records of those admitted to the university hospital with suspicion of COVID-19 in the pre-earthquake and post-earthquake periods of the pandemic. The microbiology laboratory of the hospital used reverse transcription–polymerase chain reaction (RT-PCR) results as a dependent variable and compared with the time, age and gender as the independent variables, classified according to the timing of the earthquake. RT-PCR test results were positive in 11.60% of the whole study group; however, tests were 6.69% positive before the earthquake and increased to 23.42% afterwards (P < 0.001), which equated to an increase of 4.25 times (95% CI 4.04–4.47) when comparing pre- to post-earthquake RT-PCR positivity (P < 0.001). The positive COVID-19 RT-PCR detection rate among those aged 50 and over was greater by a factor of 1.15 (95% CI 1.06–1.23) compared to a younger age group (P < 0.001). There was no difference for the gender variable used in suspected case or contact tracing applications of COVID-19 PCR. However, when stratified by age and gender, in the female group, there was a 1.24-fold (95% CI 1.11–1.38) increase in the 50+ age group (26.2%) compared to the younger age group (22.2%) after the earthquake (P < 0.001). In the male group, the rate of laboratory diagnosis of COVID-19 before the earthquake was in the under-50 age group (7.6%) as compared to the >50 age group (5.2%; P < 0.001). © 2023 CSIRO. All rights reserved.Öğe Autophagy Markers Induced by Influenza Virus and MUC1 Expressions in Cancer-Derived Cell Lines(NLM (Medline), 2023) Özkan, H.S.; Özarslan, A.; Vatansever, G.; Durmaz, S.; Çiçek, C.Influenza virus-induced autophagy is often accompanied by apoptosis and results in cell death in virus-infected cells. It is well known that autophagy is modulated by the mTOR/PI3K/Akt pathway, which plays an important role in the response to the presence of energy sources and external stimuli. This pathway is modulated by mucin 1 (MUC1), which has extracellular and intracellular components and plays an important role in metastasis and chemotherapeutic resistance. In this study, it was aimed to investigate the expression of MUC1 after the inoculation of influenza viruses into the cancer-derived cell cultures and, accordingly, the changes in autophagy markers such as mTOR and LC3B. In this study, MCF-7, HeLa and A-549 cell lines were used which have adenocarcinoma origin. To control the growth of influenza virus in these cells, the MDCK cell line was also inoculated. Centrifuge-enhanced shell-vial cell culture method was used in all experiments. Influenza A (H1N1) pdm09 strain was inoculated into these cell lines then the expressions of viral nucleic acid and cycle threshold (Ct) of MUC1, mTOR, LC3B associated genes were investigated by quantitative real-time reverse transcriptase polymerase chain reaction (qRTPCR) method in the samples taken from the supernatants of all cells at the end of the 48-hour incubation period. To investigate whether these markers were present in cells, after all cells were permeabilized with paraformaldehyde, cell-coated infected coverslips were stained with fluorescent labeled monoclonal antibodies developed against MUC1, mTOR, LC3B and influenza virus antigens. In the examination of fluorescence microscopy, all of the cell cultures (MCF-7, He-La and A-549) infected with influenza virus yielded positive results in terms of LC3B, mTOR and MUC1 monoclonal antibody staining, whereas all of the non-infected cells were found negative. Cycle threshold values of MUC1, LC3B and mTOR associated genes were found to be lower in A-549 cell line inoculated with influenza virus. Although protein expression was demonstrated in MCF-7 and He-La cell lines, similar changes were not detected in the 1/Ct values of genes in the autophagy pathway. The Ct value of the MUC1 gene was found to be higher only in the MCF-7 cell line after inoculation. In conclusion, it was observed that the specific expression pattern for influenza virus-induced autophagy was formed only in the A-549 cell line among the adenocarcinoma cells. It was thought that this relationship could constitute a dataset in further research on lung adenocarcinoma. However, in future studies, the determination of the expression of these genes at the protein level by using further tests will provide better comparison of the results.Öğe Being physically active: Perceived barriers among future doctors at Ege University, 2013-2016(Oxford Univ Press, 2018) Ergin, I.; Durmaz, S.; Hassoy, H.; Mut, A. Usturali; Varer, C.; Gecim, C.Öğe COVID-19 Infection, Vaccination, and Antibody Levels: Investigating Correlations through a Cohort Study(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Kayalı, G.A.; Durmaz, S.; Şahin, I.N.; Akkul, B.; Durusoy, R.; Akarca, F.K.; Ulukaya, S.Aim: The objective of this study was to explore the potential correlation between COVID-19 infection or vaccination and levels of anti-nucleocapsid (anti-N) and anti-spike (anti-S) antibodies. Methods: Among 6050 healthcare workers at the Ege University Hospital, a cohort study with 162 participants divided into three arms with 54 participants each was conducted. The three groups were selected as follows: those diagnosed with COVID-19 and not vaccinated (group 1), those diagnosed with COVID-19 and subsequently vaccinated with CoronaVac (group 2), and those not diagnosed with COVID-19 but vaccinated with two doses of CoronaVac (group 3). Antibody levels measured at the sixth month of follow-up were defined as the primary outcome. Results: At the sixth month, all serum samples tested positive for anti-S. Anti-S levels were found to be significantly higher in group 2 than in the other groups (p < 0.001). There were no differences in antibody levels between groups 1 and 3 (p = 0.080). Average antibody levels were found to be lower in office workers and males. Anti-N antibodies were found to be positive in 85.1% of subjects at the sixth month. In group 2, anti-N antibodies were detected in all samples at the sixth month. Anti-N antibody levels were not significantly different between groups 1 and 2 (p = 0.165). Groups 1 and 2 had significantly higher antibody levels than group 3 (p < 0.001). Conclusions: Vaccination or infection provide protection for at least 6 months. Those who have previously been diagnosed with COVID-19 do not need to be vaccinated in the early period before their antibody levels decrease. © 2023 by the authors.Öğe Prevalence and associated factors of gaming disorder among pre-clinical students in a medical school(Oxford Univ Press, 2020) Aktuna, A.; Hassoy, H.; Durmaz, S.; Durgun, K.; Sarbat, O.; Ergin, I.; Koybasi, E.[No Abstract Available]Öğe A randomized controlled study with WhatsApp embedded in smoking cessation service(Oxford Univ Press, 2018) Durmaz, S.; Ergin, I.; Durusoy, R.; Hassoy, H.; Caliskan, A.; Okyay, P.Öğe Socioeconomic inequalities in current daily smoking: Further analysis of GATS Turkey 2012(Oxford Univ Press, 2018) Hassoy, H.; Ergin, I.; Durmaz, S.
