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Öğe Central defect type partial ACL injury model on goat knees: The effect of infrapatellar fat-pad excision(SAGE Publications Ltd, 2014) Karakiliç B.; Taşkiran E.; Doğanavşargil B.; Çelik S.Objectives: The aim of this study is to investigate the primary healing capacity of the anterior cruciate ligament (ACL) and the possible effect of fat pad excision on it. For this purpose, a central defect type ACL injury model was performed. Histopathological and biomechanical studies were performed on this model. Methods: Total of ten female adolescent Anatolian Black Goats were included in the study. A midsubstance central defect was created successfully with a four mm. arthroscopic punch in the ACLs of right knees of all the subjects through a medial mini-arthrotomy and goats were assigned to groups whether the fat pad was preserved (group I, n=5) or excised completely (group II, n=5). The left knees served as control. The histopathological evaluations of the defect area were performed utilizing Hematoxylene-Eosine, Masson Trichrome, Van Gieson, and elastic Van Gieson staining as well as measurement of type I collagen immunohistochemically in one sample from each group at 10th week postoperatively. The remaining knees were evaluated biomechanically at the 12th week, measuring anterior tibial translation (ATT) of the knee joints at 90 degrees of flexion and tensile properties (Maximum Tensile Load (MTL), Maximum Elongation (ME), Stiffness (S), failure mode (FM)) of the femur-ACL-tibia complex. Statistical analysis was performed utilizing SPSS v18 package program. Mann - Whitney U and Wilcoxon’s signed rank test were used for inter and intragroup analysis, respectively. Statistical significance was set at 0.05. Results: Histopathological analysis revealed that the central defect area was fully filled macroscopically and microscopically. However, mucoid degeneration was observed in group II. Relative collagen type I content increased in group II. There was no significant difference both within and between groups in terms of ATT values (p=0.715 and p=0.149 respectively). There was no statistical significance between and within groups in terms of MTL and ME; however group II demonstrated greater stiffness than group I (p=0,043) Overall (n=16 knees), tibial avulsion was the commonest mode of failure (n=9) in both control and operated knees. Conclusion: These findings revealed that the central defect type partial ACL injury model acted stable on A-P direction and had full healing capacity. The excision of the fat-pad had no additional effect except increased stiffness. Tibial insertion site seemed to be the weakest portion of the femur-ACL-tibia complex in adolescent goat knees. © The Author(s) 2014.Öğe Cystic bone lesions: Histopathological spectrum and diagnostic challenges [Kemiğin Kistik Lezyonları: Histopatolojik Spektrum ve Tanısal Güçlükler](Federation of Turkish Pathology Societies, 2015) Doğanavşargil B.; Ayhan E.; Argin M.; Pehlivanoğlu B.; Keçeci B.; Sezak M.; Başdemir G.; Öztop F.Objective: Bone cysts are benign lesions occurring in any bone, regardless of age. They are often asymptomatic but may cause pain, swelling, fractures, and local recurrence and may be confused with other bone lesions. Material and Method: We retrospectively re-evaluated 143 patients diagnosed with aneurysmal bone cyst (n=98, 68.5%), solitary bone cysts (n=17 11.9%), pseudocyst (n=10.7%), intraosseous ganglion (n=3, 2.1%), hydatid cyst (n=2; 1.4), epidermoid cyst (n=1, 0.7%) and cysts demonstrating “mixed” aneurysmal-solitary bone cyst histology (n=12, 8.4%), and compared them with nonparametric tests. Results: Aneurysmal bone cyst, solitary bone cysts and mixed cysts were frequently seen in the first two decades of life while the others occurred after the fourth decade. Aneurysmal bone cysts, intraosseous ganglion and pseudocysts were more common in women contrary to solitary bone cyst and mixed cysts (the female/male ratio was 1.22, 2 and 1.5 versus 0.7 and 0.5, respectively). Aneurysmal bone cyst, solitary bone cysts and “mixed” cysts were mostly seen in long bones, predominantly the femur, while epidermoid, hydatid and pseudocysts were all seen in fat bones like the vertebra, pelvis and mandible (p=0.001, chi-square). repeat biopsies were performed in 19 cases (13.3%), 84.2% of which were aneurysmal bone cyst (5 conventional, 9 solid, 1 secondary and 1 subperiosteal) and three (15.8%) were mixed cysts (p=0.02, chi-square). Notably, some of them were located in inaccessible areas of pelvis (n=3), femur (n=3) and maxilla (n=2). Conclusion: The most common and challenging intraosseous cysts are aneurysmal bone cysts, particularly the “solid” variant. The “mixed” aneurysmal-solitary bone cyst “subgroup” requires further research with larger series to be defined more thoroughly. © 2015, Federation of Turkish Pathology Societies. All Rights Reserved.Öğe Evaluation of the role of Epstein-Barr virus in cases of nodal or extranodal T- and NK-cell lymphoma using eber in situ hybridization(Versalius University Medical Publisher, 2015) Karaarslan S.; Hekimgil M.; Soydan S.; Ertan Y.; Doğanavşargil B.Various racial and geographic differences have been observed in studies questioning the role of Epstein-Barr virus (EBV) infection in the etiology of T- and NK-cell lymphomas. The aim of this study was to evaluate the relationship of EBV with nodal or extranodal (skin excluded) T- and NK-cell lymphoma subtypes encountered in our geographic area. Sixty-two cases of peripheral T-cell lymphoma were included in the study. EBV-encoded early RNA (EBER) was detected by in situ hybridization. The distributions of T- and NK-cell lymphoma subtypes were as follows: 32 peripheral T-cell lymphomas, unspecified (PTCL, NOS), 13 anaplastic large-cell lymphomas (ALCL), 8 angioimmunoblastic T-cell lymphomas (AITCL), 4 extranodal NK/T-cell lymphomas, nasal type (NKTCL), 3 enteropathy-type T-cell lymphomas (ETTCL), 1 hepatosplenic T-cell lymphoma (HSTCL), and 1 subcutaneous panniculitis-like T-cell lymphoma (SPTCL). Using a cut-off value of > 25% of EBER-positive neoplastic lymphoid cells, EBV was positive in 22.6% of all cases. According to subtype, the neoplastic cells of 31.3% of PTCL, NOS and 100% of extranodal NKTCL, nasal type were EBER positive, whereas some cases of ALCL, AITCL, and ETTCL presented EBER-positive non-neoplastic cells, and all cells of HSTCL and SPTCL were EBV negative. Extranodal NKTCL, nasal type, presented the strongest association with EBV, followed by PTCL, NOS. © 2015, Versalius University Medical Publisher. All rights reserved.Öğe Gastrointestinal parasitosis: Histopathological insights to rare but intriguing lesions of the gastrointestinal tract(Federation of Turkish Pathology Societies, 2016) Pehlivanoğlu B.; Doğanavşargil B.; Sezak M.; Nalbantoğlu İ.; Korkmaz M.Objective: Gastrointestinal parasitosis is a significant cause of morbidity and mortality. Definitive diagnosis is usually made by stool tests and/or serology but may require tissue evaluation. Although pathologists are usually familiar with common parasites, it is not well established whether the diagnosis could be suspected without seeing the “parasite” itself. Material and Method: Resection or biopsy specimens of 32 cases with Giardia intestinalis (n=20), Enterobius vermicularis (n=5), Entamoeba histolytica (n=4), Fasciola hepatica (n=1), Strongyloides spp. (n=1) and Taenia saginata (n=1) infections were retrospectively re-evaluated for accompanying mucosal changes, and compared with nonparametric tests. Results: The most common changes were congestion (65.6%) and eosinophilic infiltration (50%). Chronic active mucosal inflammation accompanied 37.5% of the cases. More than 10 eosinophils/HPF were present in 43.8%. Only one case of G. intestinalis, E. vermicularis, E. histolytica, and F. hepatica showed more than 50 eosinophils/HPF. Mucosal architectural abnormalities were present in 34.4%. Granulomas, giant cells and Charcot-Leyden crystals were only seen accompanying F. hepatica. No statistically significant difference was found between parasite subspecies regarding presence of inflammation, lymphoid aggregates, architectural distortion, congestion, ulceration and increase of eosinophils. Conclusion: Parasites induce nonspecific inflammation, slight mucosal architectural changes, mild eosinophilic infiltrate or granuloma formation. They may cause ulceration, bowel obstruction or perforation. Parasitosis should also be considered when evaluating cases mimicking inflammatory bowel disease, celiac disease or those that do not fulfill diagnostic criteria. © 2016, Federation of Turkish Pathology Societies. All Rights Reserved.Öğe Kaleidoscopic views in the bone marrow: Oxalate crystals in a patient presenting with bicytopenia [Kemik İliğinde oksalat kristalleri birikimine bağlı gelişen bisitopeni](Turkish Society of Hematology, 2016) Dere Y.; Erbil S.; Sezak M.; Doğanavşargil B.; Yılmaz M.; Özsan N.; Hekimgil M.[No abstract available]Öğe Structural changes in the intestinal mucosa of wistar-albino rats after irradiation, and protective effect of ascorbic acid [Wistar-albino sıçanlarda işınlama sonrası İntestinal mukozada oluşan değişiklikler ve askorbik asidin koruyucu etkisi](Turkiye Klinikleri, 2014) Aydin B.; Yalman D.; Doğanavşargil B.; Kamer S.; Ellidokuz H.Objective: To investigate the histopathological changes and the protective effect of ascorbic acid on the intestinal mucosa of the Wistar-albino rats after irradiation. Material and Methods: Thirty-two young-adult, Wistar albino rats were randomized into control, vitamin C only, radiotherapy only, and radiotherapy + vitamin C groups. Vitamin C (250 mg/kg/day) was added to the drinking water of the rats in vitamin C groups for 10 days. A single dose of 8 Gy radiotherapy was applied to the abdominal region of the rats on the eleventh day. The rats were sacrificed after 72 hours, and their all gastrointestinal tract, from distal esophagus to the anus, was removed. A 1-cm segment selected from a 5-cm proximal part of ilioceacal valve from the ileum and 5 cm distal part from the colon were analyzed regarding crypt count, abnormal epithelial cells, mucosal congestion, edema, cryptic tissue changes, villus height, structural changes, degradation and union of the villi, subepithelial detachment, and mitotic and apoptotic figure counts in the basement membrane. Results: In the radiotherapy only group; crypt count decreased, apoptotic figure count increased, the villi showed extreme degradation, union and shortening, there were lymphocyte infiltration and epithelial cells in the lumen. In the radiotherapy + vitamin C group; apoptotic figure count was very low, villus structure, cryptic and mucosal layer were preserved partially. When radiotherapy only group was compared to radiotherapy + vitamin C group, villus height differed in favor of radiotherapy + vitamin C group (p=0.001), however no differences regarding mitotic count, apoptotic figure and crypt count were found (p=0.03, p=0.524, p=0.772, respectively). Conclusion: Vitamin C protects intestinal mucosa from radiation damage, and ascorbic acid may not reduce the radiation-induced antitumor activity. Prophylactic use of vitamin C in pelvic irradiation can prevent the side effects. However, it could act as a pro-oxidant in higher doses, therefore dose-controlled studies are needed in tumor tissues. © 2014 by Türkiye Klinikleri.
