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Öğe Applicability evaluation of mtDNA based molecular identification in mosquito species/subspecies/biotypes collected from Thessaloniki, Greece(Elsevier B.V., 2023) Köseoğlu, A.E.; Paltacı, S.; Can, H.; Giantsis, I.A.; Güvendi, M.; Demir, S.; Döşkaya, M.The genus Culex, containing many described species, plays a role as a vector for diseases of medical and veterinary importance worldwide. Among these species, Culex pipiens is one of the most widespread mosquitoes and is classified into two biological forms (biotypes), named as Culex pipiens pipiens and Culex pipiens molestus. Due to similar morphological structure between these biotypes, morphological identification is inadequate. Thus, molecular methods have been developed and are considered more reliable, some of which are based on analyses of mitochondrial DNA. The aim of the present study was to evaluate the applicability and reliability of mtDNA based molecular identification methodologies. Initially, mosquito specimens (n = 100) collected from Thessaloniki, Greece were morphologically analyzed. Then, mitochondrial cox1 sequencing and PCR-RFLP methods were used to confirm the morphological identification results as well as to discriminate species and subspecies/biotype of Culex pipiens complex. According to morphological identification results, Culex pipiens complex (n = 92), Culex modestus (n = 6) and Culex theileri (n = 2) were detected. Using mtDNA sequencing, all Culex modestus and Culex theileri samples were confirmed whereas 86 of Culex pipiens complex were detected as Culex pipiens but surprisingly the remaining six of them were detected as Culex quinquefasciatus. Among Culex pipiens specimens, PCR-RFLP detected that frequency of Culex pipiens pipiens (85%; 85/100) was very high compared to Culex pipiens molestus (1%, 1/100). In conclusion, this study shows the necessity of use of molecular methods beside morphological methods for especially specimens detected as Culex pipiens. Also, it was shown that mtDNA PCR-RFLP methodology represents a well-established alternative for Culex biotypes identification. © 2023 Elsevier B.V.Öğe Cattle Fever Tick, Rhipicephalus annulatus (Acari: Ixodidae), and the Quest for Discovery of Its Natural Enemies in the Balkan Region(Cabi Publishing-C A B Int, 2017) Kashefi, J.; Demir, S.; Goolsby, J.; Smith, L.; Chaskopoulou, A.; Mason, PG; Gillespie, DR; Vincent, CÖğe Composition of the essential oils of Centaurea aphrodisea, C. polyclada, C. athoa, C. hyalolepis and C. iberica(Georg Thieme Verlag Kg, 2011) Erel, Baykan S.; Demirci, B.; Demir, S.; Karaalp, C.; Baser, K. H. C.Öğe CYTOTOXIC AND ANTIMICROBIAL ACTIVITIES OF PTILOSTEMON CHAMAEPEUCE (L.) LESS(University of Ankara, 2022) Demir, S.; Erzurumlu, Y.; Öztürk, İ.; Ballar, Kirmizibayrak, P.; Karaalp, C.Objective: The aim of this study is to investigate the antimicrobial and cytotoxic activities of n-hexane chloroform and methanol extracts prepared from the aerial parts of P. chamaepeuce (L.) Less. (Asteraceae). Material and Method: The antimicrobial activities of the extracts on standard bacterial strains (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus subtilis and Streptococcus pneumoniae) and fungal strains (Candida albicans and C. parapsilosis) were examined by microdilution method and minimum inhibitory concentration (MIC) values were determined. The cytotoxic activity of the extracts was investigated by cell proliferation assay using WST-1 reagent on various cancer cell lines including HeLa, U2OS, PC3, MCF-7, and A549 cancer cell lines. Result and Discussion: The MIC values of n-hexane, chloroform and methanol extracts were between 250-1000 µg/mL against S. aureus, E. faecalis and B. subtilis. n-hexane and chloroform extracts had varying levels of cytotoxic activity in all tested cancer cell lines (IC50: 21.0-67.1 ?g/mL). © 2022 University of Ankara. All rights reserved.Öğe Cytotoxic properties of five Centaurea L. species from Anatolia(Georg Thieme Verlag Kg, 2011) Erel, Baykan S.; Demir, S.; Kose, Aydin F.; Ballar, P.; Karaalp, C.Öğe Entomological Survey for the Detection of Sand Fly Fauna and Vector Species in the Cutaneous Leishmaniasis Endemic Area in East Mediterranean Region of Turkey, Mersin Province(NLM (Medline), 2020) Limoncu, M.E.; Balcıoğlu, İC.; Töz, S.; Demir, S.; Kavur, H.; Karakuş, M.; Özbel, Y.Cutaneous (CL) and visceral (VL) forms of leishmaniasis, transmitted by sand flies, are seen in all countries located in Mediterranean Basin including Turkey. In this study, we aimed to conduct an entomological survey for the detection of sand fly fauna and vector species in Mersin province, one of the important endemic areas for CL in Turkey. In total, 912 sand fly specimens were collected in 2010 and 2011 using CDC light traps. Nine Phlebotomus (Diptera: Psychodidae) and three Sergentomyia (Diptera: Psychodidae) species were detected. Of the collected Phlebotomus sand flies, P. sergenti Parrot, 1917 (30.1%) was the most dominant followed by P. alexandri Sinton, 1928 (18.2%), P. neglectus/syriacus Tonnoir Adler (12.0%), P. tobbi Adler & Theodor, 1930 (11.7%), and P. papatasi Scopoli, 1786 (10.2%), while S. minuta Rondani, 1843 (11.3%) was the dominant species among Sergentomyia. During the field work in 2011, female specimens (n = 81) were screened for the presence of Leishmania promastigotes by midgut dissection, and all were found negative. The rest of the collected female specimens (n = 334) were pooled according to species (P. alexandri, P. neglectus/syriacus, P. papatasi, P. sergenti, P. simici, and P. tobbi) and location (Mut, Silifke, and Anamur). In total, 29 pools were generated and real-time ITS1 PCR assay was performed to detect and identify natural Leishmania Ross, 1903 (Kinetoplastida: Trypanosomatida) infection. Two pools, both from Mut town, containing P. sergenti specimens were found positive and Leishmania tropica Ross, 1903 was identified as an infectious agent for both pools. In conclusion, the sand fly fauna was determined in an endemic area for CL. The detection of L. tropica DNA in P. sergenti specimens showed the possible vectorial role of this species in Mersin province. © Crown copyright 2020.Öğe Helminth parasites of the levantine frog (Pelophylax bedriagae Camerano, 1882) from the western part of Turkey(Walter De Gruyter Gmbh, 2015) Demir, S.; Yakar, O.; Yildirimhan, H. S.; Birlik, S.Fifty-four Pelophylax bedriagae (Levantine Frog) from Turkey (Izmir and Manisa Provinces) were examined for helminth parasites. The frogs were collected between 2012 and 2014 years. Eight species of helminth parasites were recorded: 3 species of Nematoda (Rhabdias bufonis, Cosmocerca ornata, Oswaldocruzia filiformis), 3 species of Digenea (Diplodiscus subclavatus, Haematoloechus breviansa, Gorgoderina vitelliloba), 1 species of Acanthocephala (Acanthocephalus ranae) and 1 species of Hirudinea (Hirudo medicinalis). Pelophylax bedriagae is a new host record for these parasite species.Öğe Helminth parasites of the levantine frog (Pelophylax bedriagae Camerano, 1882) from the western part of Turkey(Walter De Gruyter Gmbh, 2015) Demir, S.; Yakar, O.; Yildirimhan, H. S.; Birlik, S.Fifty-four Pelophylax bedriagae (Levantine Frog) from Turkey (Izmir and Manisa Provinces) were examined for helminth parasites. The frogs were collected between 2012 and 2014 years. Eight species of helminth parasites were recorded: 3 species of Nematoda (Rhabdias bufonis, Cosmocerca ornata, Oswaldocruzia filiformis), 3 species of Digenea (Diplodiscus subclavatus, Haematoloechus breviansa, Gorgoderina vitelliloba), 1 species of Acanthocephala (Acanthocephalus ranae) and 1 species of Hirudinea (Hirudo medicinalis). Pelophylax bedriagae is a new host record for these parasite species.Öğe Helminth parasites of the levantine frog (Pelophylax bedriagae Camerano, 1882) from the western part of Turkey(Walter De Gruyter Gmbh, 2015) Demir, S.; Yakar, O.; Yildirimhan, H. S.; Birlik, S.Fifty-four Pelophylax bedriagae (Levantine Frog) from Turkey (Izmir and Manisa Provinces) were examined for helminth parasites. The frogs were collected between 2012 and 2014 years. Eight species of helminth parasites were recorded: 3 species of Nematoda (Rhabdias bufonis, Cosmocerca ornata, Oswaldocruzia filiformis), 3 species of Digenea (Diplodiscus subclavatus, Haematoloechus breviansa, Gorgoderina vitelliloba), 1 species of Acanthocephala (Acanthocephalus ranae) and 1 species of Hirudinea (Hirudo medicinalis). Pelophylax bedriagae is a new host record for these parasite species.Öğe Molecular screening of Leishmania spp. infection and bloodmeals in sandflies from a leishmaniasis focus in southwestern Turkey(Wiley, 2017) Karakus, M.; Pekagirbas, M.; Demir, S.; Eren, H.; Toz, S.; Ozbel, Y.Leishmaniasis is an arthropod-borne disease that affects approximately 2 million people worldwide annually. The aims of this study were to detect the presence of Leishmania (Kinetoplastida: Trypanosomatidae) DNA and the feeding preferences of probable vector species in an endemic focus of Leishmania infantum in Turkey. Entomological sampling was performed in August and October 2015 in Aydn province, where cases of human and canine leishmaniasis have been reported previously. A total of 1059 sandfly specimens comprising nine species belonging to two genera, Phlebotomus and Sergentomyia (both: Diptera: Psychodidae), and five subgenera of the Phlebotomus genus (Phlebotomus, Paraphlebotomus, Larroussius, Adlerius and Transphlebotomus) were collected in five villages. Among all Phlebotomus specimens, Phlebotomus neglectus (39%) was noted as the most abundant species, followed by Phlebotomus tobbi (18%). LeishmaniaDNA was detected in pools from P.neglectus, P.tobbi and Sergentomyia dentata by kDNA polymerase chain reaction (PCR). LeishmaniaDNA from Phlebotomus specimens was identified as L.infantum, but LeishmaniaDNA from Sergentomyia spp. could not be identified to species level by ITS-1 real-time PCR. The detection of LeishmaniaDNA in wild-caught P.neglectus and the high percentage (24.2%) of human DNA in engorged specimens suggests that P.neglectus is probably an important vector species for L.infantum in Aydn province.Öğe Psychiatric and Clinical Characteristics of Hereditary Angioedema Patients Who Experienced Attacks During COVID-19(Esmon Publicidad S A, Dept Allergy & Clin Immunol, Clin Univ Navarra, 2021) Gokmen, Mete N.; Tuncel, Kuman O.; Bogatekin, G.; Bulut, G.; Demir, S.; Gelincik, A.; Dalgic, Tunakan C.[No Abstract Available]Öğe sandflyDST: a dynamic web-based decision support tool for the morphological identification of sandflies present in Anatolia and mainland Europe, and user study(Wiley, 2016) Karakulah, G.; Karakus, M.; Suner, A.; Demir, S.; Arserim, S. K.; Toz, S.; Ozbel, Y.Species identification of sandflies is mainly performed according to morphological characters using classical written identification keys. This study introduces a new web-based decision support tool (sandflyDST) for guiding the morphological identification of sandfly species present in Anatolia and mainland Europe and classified in the Phlebotomus and Sergentomyia genera (both: Diptera: Psychodidae). The current version of the tool consists of 111 questions and 36 drawings obtained from classical written keys, and 107 photographs for the quick and easy identification of 26 species of the genus Phlebotomus and four species of the genus Sergentomyia. The tool guides users through a decision tree using yes/no questions about the morphological characters of the specimen. The tool was applied by 30 individuals, who then completed study questionnaires. The results of subsequent analyses indicated that the usability ((x) over bar (SUS Score) = 75.4) and users' level of appreciation (86.6%) of the tool were quite high; almost all of the participants considered recommending the tool to others. The tool may also be useful in training new entomologists and maintaining their level of expertise. This is a dynamic tool and can be improved or upgraded according to feedback. The tool is now available online at http://parasitology.ege.edu.tr/sandflyDST/index.php.Öğe Secondary metabolites of Centaurea depresso Bieb(Georg Thieme Verlag Kg, 2009) Demir, S.; Karaalp, C.; Bedir, E.
