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Öğe The 1st Turkish in vitro diagnostic symposium evaluation(Wiley-Blackwell, 2016) Kose, F. Aydin; Celebi, S.; Cengiz, H.; Harmanci, D.; Kocak, A.; Canbay, E.; Aslan, D.; Sezer, E.; Pabuccuoglu, A.; Kocdor, H.Öğe The decision making levels of urine tetrasaccharide for diagnosis of Pompe disease(Elsevier, 2019) Canbay, E.; Vural, M.; Ucar, S. Kalkan; Sezer, E. DemIrel; Karasoy, H.; Yuceyar, A.; Sozmen, E.[No abstract available]Öğe Development an amperometric microbial-enzyme hybrid cholesterol biosensor based on ionic liquid MWCNT carbon paste electrode(Wiley, 2018) Canbay, E.; Akyilmaz, E.Öğe Development, optimization and validation of LC-MS/MS method for the determination of DBS GALT enzyme activity(Academic Press Inc., 2023) Topbas, M.; Canbay, E.; Sezer, E.; Canda, E.; Kalkan, Uçar, S.; Çoker, M.; Yildirim, Sözmen, E.Galactosemia is a carbohydrate metabolism disorder often caused by galactose-1-phosphate uridyl transferase (GALT) deficiency. Detecting GALT deficiency involves measuring intra-erythrocyte enzyme activity. We aimed to create a robust liquid chromatography-mass spectrometry (LC-MS/MS) method to assess GALT activity in dried blood spot (DBS) samples. We validated this method and compared it to the fluorometric approach. We investigated the impact of K2EDTA and lithium heparin tubes on enzyme activity to identify the best sample collection tube. We also assessed the reaction-stopping method. The developed approach employed [13C6]-galactose-1-phosphate as a substrate and UDP-N-acetylglycosamine as an internal standard (IS). The mean ± SD value for GALT activity of DBS samples was determined as 6.37 ± 1.96 ?mol/gHb/hour. The linear range was 0.4–50 ?M (2.4–310% of normal) in the DBS method. The % coefficient of variation (%CV) values were less than 15 for intra-day and inter-day repeatability studies. Over 90% recovery was achieved in recovery studies, and no ion suppression from matrix was detected. DBS samples were quite stable for 31 days under different storage conditions. Enzyme activity results reported as <3.5 U/g Hb by fluorometric method, were quantitatively determined for even very low concentrations by LC-MS/MS method. © 2023 Elsevier Inc.Öğe Evaluation of anti-inflammatory potential and LC-MS/MS analysis of different standards(Wiley, 2018) Sozmen, A. B.; Canbay, E.; Sozmen, E. Yildirim; Ovez, B.Öğe Glycosaminoglycan-induced proinflammatory cytokine levels as disease marker in mucopolysaccharidosis(Academic Press, 2024) Çopur, O.; Yazıcı, H.; Canbay, E.; Durmaz, B.; Canda, E.; Ucar, S.K.; Coker, M.Recently, it has been shown disturbances in oxidant/antioxidant system and increases in some inflammatory markers in animal studies and in some Mucopolysaccharidoses (MPSs) patients. In this study, we aimed to determine the oxidative stress/antioxidant parameters and pro-inflammatory cytokine levels in the serum of MPS patients, in order to evaluate the possible role of inflammation in these patient groups regarding to accumulated metabolites. MPS I (n = 3), MPS II (n = 8), MPS III (n = 4), MPS IVA (n = 3), MPS VI (n = 3), and VII (n = 1) patients and 20 age-matched healthy subjects were included into the study. There was no statistically significant change in activities of SOD, Catalase, GSH-Px and lipid peroxidation levels in erythrocytes between the MPS patients and healthy controls. While IL-1alpha (p = 0.054), IL-6 (p = 0.008) levels, and chitotriosidase activity (p = 0.003) elevated in MPS3 patients, IL1? (p = 0.006), IL-1? (p = 0.006), IL-6 (p = 0.006), IFN? (p = 0.006), and NF?B (p = 0.006) levels increased in MPS-6 patients. Elevated levels of IL-6, IL1? and chitotriosidase activity demonstrated macrophage activation in MPSIII untreated with enzyme replacement. Our study showed for the first time that high levels of IL1?, IL-6, IL1? and NF?B were present in MPSVI patients, demonstrating the induction of inflammation by dermatan sulphate. The low level of paraoxonase in MPSVI patients may be a good marker for cardiac involvement. Overall, this study provides important insights into the relationship between lysosomal storage of glycosaminoglycan and inflammation in MPS patients. It highlights possible pathways for the increased release of inflammatory molecules and suggests new targets for the development of treatments. © 2023 Elsevier LtdÖğe How preeclampsia affects oxidant status and antiinflammatory potential of breast milk?(Wiley-Blackwell, 2016) Demir, D.; Turan, V.; Ozturk, S.; Canbay, E.; Sezer, E.; Sozmen, E.Öğe Investigation of utility of homocystine as an internal standard for the determination of leukocyte cystine level by LC MS MS method(Wiley, 2017) Canbay, E.; Sezer, E.; Sozmen, E. YildirimÖğe Phenolic content and potential anti-inflammatory effects of Myrtus communis L. products(Wiley-Blackwell, 2016) Ozturk, S.; Canbay, E.; Yildirim, H. K.; Sozmen, E. Y.Öğe Simultaneous determination of epinephrine and dopamine by using Candida tropicalis yeast cells immobilized in a carbon paste electrode (CPE) modified with single wall carbon nanotube (SWCNT)(Wiley-Blackwell, 2016) Canbay, E.; Akyilmaz, E.; Dinckaya, E.; Guvenc, C.; Yasa, I.; Bayram, E.Öğe A specific, sensitive, simplified and fast LC-MS/MS method for plasma Lyso-GB3 levels(Wiley, 2019) Canbay, E.; Sezer, E.; Ucar, S. Kalkan; Coker, M.; Sozmen, E.[No abstract available]Öğe Validation of a new LC-MS/MS method for serum/saliva cortisol measurement and comparison with commercial ELISA/CLIA methods(Elsevier, 2019) Canbay, E.; Sezer, E. D.; Gurkan, F. T.; Gozen, O.; Koylu, E. O.; Sozmen, E. Y.[No abstract available]
