Yazar "Baka, Meral" seçeneğine göre listele
Listeleniyor 1 - 20 / 27
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Acute hypoxia exposure following prenatal stress impairs hippocampus and novelty-seeking behavior in adolescent rats(Wiley, 2022) Celik, Kubra; Bilim, Petek; Garip, Gurur; Durmaz, Burak; Sozmen, Eser Yildirim; Baka, MeralObjectives The present study aimed to investigate the effects of acute hypoxia exposure following prenatal stress on the novelty-seeking behavior and hippocampus of adolescent rats. Methods The offspring were divided into prenatal stress (PS) and non-stress (NS) groups. Both groups were exposed to hypoxia on postnatal day 10 (P10) while control groups were undisturbed. Novel object recognition task was performed in each group. Next, brains were collected to examine hippocampus via immunohistochemical and biochemical studies on postnatal day 35 (P35). Results PS decreased novelty discrimination and synaptophysin (SYN) expressions in both CA1 and CA3 of the hypoxia group prominently (p < 0.05). Nestin-expressing cells were reduced while vascular endothelial growth factor (VEGF) expression was enhanced in the subgranular zone (SGZ) of PS-hypoxia group (p < 0.05). VEGF enhancement triggered angiogenesis in the CA1 and CA3 significantly (p < 0.05). PS also increased thiobarbituric acid reactive substances (TBARS) levels in the hypoxia group as a result of oxidative stress (p < 0.05). Conclusion These findings demonstrated that PS exacerbates neurodevelopmental deficits in the hippocampus of acute hypoxia-induced offspring in adolescence.Öğe Amino acid neurotransmitter levels in the cerebral cortex of mice receiving imipenem / cilastatin-lack of excitotoxicity in the central nervous system(1998) Akısü, Mete; Kültürsay, Nilgün; Çoker, Canan; Akısü, Çiler; Baka, Meral[Özet Yok]Öğe A cultivating experience of Ege University Faculty of Medicine with research training program AEP(2016) Sezer, Ebru; Batı, Ayşe Hilal; Mandıracıoğlu, Aliye; Öcek, Zeliha Aslı; Çiçek, Candan; Baka, Meral; Evren, Vedat…Öğe Effects of 2,4-dichlorophenoxyacetic acid formulation on medulla spinalis of Poecilia reticulata: A histopathological study(Pergamon-Elsevier Science Ltd, 2009) Uyanikgil, Yigit; Yalcinkaya, Murat; Ates, Utku; Baka, Meral; Karakisi, HaticeThis study investigated the possible effects of a commonly used foliar herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) formulation on medulla spinalis of lebistes. Fish were exposed to 2,4-D (15, 30, 45 ring L-1), behavioral changes were monitored. Fish were fixed, histopathological examination was carried out on sections taken from the upper parts of the fish body. Histopathology showed increase in neuronal loss, swelling indicating formation of intracellular edema, vacuolization noticed as the formation of vacuoles within or adjacent to cells, deformation in the Nissl granules, pyknosis and gliosis in medulla spinalis. Behavioral changes were decreased general activity, grouping, shortness in breath. sudden rotations and jumping, loss of equilibrium and colour. In conclusion, this commercial formulation of 2,4-D is considerably neurotoxic to lebistes. Fish constitute the last link in the chain of the feeding cycle in aquatic eco-system, number of studies investigating acute and chronic neurotoxicity of various herbicides in fish should be increased. (C) 2009 Elsevier Ltd. All rights reserved.Öğe Effects of 2,4-dichlorophenoxyacetic acid formulation on medulla spinalis of Poecilia reticulata: A histopathological study(Pergamon-Elsevier Science Ltd, 2009) Uyanikgil, Yigit; Yalcinkaya, Murat; Ates, Utku; Baka, Meral; Karakisi, HaticeThis study investigated the possible effects of a commonly used foliar herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) formulation on medulla spinalis of lebistes. Fish were exposed to 2,4-D (15, 30, 45 ring L-1), behavioral changes were monitored. Fish were fixed, histopathological examination was carried out on sections taken from the upper parts of the fish body. Histopathology showed increase in neuronal loss, swelling indicating formation of intracellular edema, vacuolization noticed as the formation of vacuoles within or adjacent to cells, deformation in the Nissl granules, pyknosis and gliosis in medulla spinalis. Behavioral changes were decreased general activity, grouping, shortness in breath. sudden rotations and jumping, loss of equilibrium and colour. In conclusion, this commercial formulation of 2,4-D is considerably neurotoxic to lebistes. Fish constitute the last link in the chain of the feeding cycle in aquatic eco-system, number of studies investigating acute and chronic neurotoxicity of various herbicides in fish should be increased. (C) 2009 Elsevier Ltd. All rights reserved.Öğe Effects of Melatonin on the Cerebellum of Infant Rat Following Kaolin-Induced Hydrocephalus: a Histochemical and Immunohistochemical Study(Springer, 2017) Uyanikgil, Yigit; Turgut, Mehmet; Baka, MeralHydrocephalus is a developmental disorder causing abnormally collected cerebrospinal fluid within the cerebral ventricles. It leads to bigger skulls and many dysfunctions related to the nervous system. Here, we addressed whether exogenous melatonin administration could reverse the clinical features of kaolin-induced hydrocephalus in infantile rats. A controlled double-blinded study was conducted in 2-week-old 45 Wistar albino rats, which were divided into three groups: Group A, the control group, received intracisternal sham injection with solely the needle insertion; group B, the hydrocephalus group, was treated with isotonic NaCl after kaolin injection; and group C, the hydrocephalus + melatonin group, was given i.p. exogenous melatonin at a dose of 0.5 mg/100 g body weight after kaolin injection. Histological and immunohistochemical analyses were performed after the induction of hydrocephalus and melatonin administration. Glial fibrillary acidic protein was stained by immunohistochemical method. TUNEL method was used to define and quantitate apoptosis in the cerebellar tissues. Statistical analysis was performed by nonparametric Kruskal-Wallis H test, and once significance was determined among means, post hoc pairwise comparisons were carried out using Mann-Whitney U test. We found that melatonin administration significantly ameliorated ratio of substantia grisea area/substantia alba area in the cerebellum of infantile rats. Histologically, there was a significant reduction in the number of cerebellar apoptotic cells after the hydrocephalus induced by kaolin (P < 0.05). Our results clearly revealed that the histopathological changes in the cerebellum were reversed by systemic melatonin administration in infantile rats with kaolin-induced hydrocephalus. Nevertheless, further studies are needed to suggest melatonin as a candidate protective drug in children with hydrocephalus.Öğe Efficacy of melatonin on offspring liver maturation in pinealectomized pregnant rats subjected to experimental epilepsy(Blackwell Publishing, 2007) Ates, Utku; Baka, Meral; Uyanikgil, Yigit; Turgut, Mehmet; Yavasoglu, Altug; Yurtseven, MineBackground and Aims: In clinical practice, maternal epilepsy is a disabling disease for newborn infants, but current data concerning the effect of epileptic phenomena in pregnant mothers on newborns are still limited. This study was undertaken to investigate the effects of pinealectomy (Px) and melatonin treatments on the morphological changes in the liver tissue of newborn rats following experimental epilepsy during pregnancy. Methods: Female Swiss Albino rats were divided into five groups: intact control group; saline control group; epilepsy group; epilepsy plus Px group; and melatonin-treated epilepsy plus Px group. At one month after Px, an acute grand mal epileptic seizure was induced by penicillin-G during their pregnancy in all animals except the control groups. On the neonatal first day, newborn rats were perfused with intracardiac fixative solution, and then livers were removed and processed for toluidine blue, periodic acid-Schiff (PAS) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) reactivity. Results: Normal migration and hepatic maturation were determined in the postnatal rat liver in the control groups, while the morphological structure of the liver in the epilepsy and epilepsy plus Px groups corresponded to the early embryonic period. In the melatonin-treated epilepsy plus Px group, the number of TUNEL positive cells decreased significantly compared to both epilepsy and epilepsy plus Px groups; however, there was no statistically significant difference from the control groups as a result of melatonin activity. Conclusions: Some histological findings consistent with chronic fetal distress in newborns of mother rats with epilepsy and Px were observed. Melatonin could be a candidate protective drug for the development of liver tissue in pregnant patients with epilepsy.Öğe Epidural lornoxicam administration - innocent(Elsevier Sci Ltd, 2007) Canduz, Basak; Aktug, Huseyin; Mavioglu, Oemuer; Erkin, Yueksel; Yilmaz, Ozlem; Uyanikgil, Yigit; Korkmaz, Huelya; Baka, MeralWe aimed to determine the analgesic efficacy and clincial or histopathological neurotoxicity of epidural single-dose lornoxicam. Caudal epidural catheters were inserted into 28 rabbits, divided into four groups, on day 1. Pain latency and degree of motor and sensory loss for each animal for different concentrations of lornoxicam were determined on day 2. All animals were sacrificed on day 3 and laminectomy was performed. Five-mu m thick sections of spinal cord, obtained from two segments caudal and two segments rostral from tip of the catheter, were fixed and were stained and evaluated by light microscopy. Lornoxicam produced dose-dependent analgesia (increase in pain latency), brief, mild and reversible motor and sensory block, and histopathological signs of neurotoxicity. Clinical application of epidural lornoxicam should proceed with caution. (c) 2006 Elsevier Ltd. All rights reserved.Öğe Epileptik erişkin ratlar ve onlara ait yenidoğan rat beyinlerinde GABAa ve GABAb reseptörlerinin ultrastrüktütel dağılımı ile böbrek ve karaciğer ultrastrüktür ilişkileri(Ege Üniversitesi, 2002) Baka, MeralGünümüze kadar yapılan çalışmalarda, epileptik annelere ait yenidoğanların klinik bakılarını gösteren ve alınacak önlemleri içeren çalışmalar yetersizdir. Dünya standartlarına uygun sağlıklı yeni nesiller oluşturma hedeflerine katkıda bulunmak adına hamile epileptik deneysel modeliyle yenidoğan cerebrumlarının incelenmesi amaçlandı. Bu araştırmada 225-250 gr ağırlığında genç sıçanlara hamileliğin 13.gününde stereotaksi aleti ile intrahippocampal CA3 bölgesine 400 IU penisilin-G uygulaması yapılarak sıçanlar (n=6) akut grand mal epileptik nöbet geçirilmeleri sağlandı.İkinci gruba intrahippokampal serum fizyolojik uygulanarak sham (n=6) kabul edildi. Üçüncü grup ise kontrol olarak cerrahi işlem yapılmadan bırakıldı (n=6) . Kafeslere ayrılan sıçanların doğum yapması beklendi. Neonatal 1. gündeki sıçanlara(P1) letal doz anestezi altında intrakardiyak perfüzyon ile tespit edilen yenidoğan cerebellumları ışık ve elektron mikroskopik olarak değerlendirildi. Kontrol ve serum fizyolojik uygulanan deneklerin yenidoğan gruplarında, yenidoğan cerebellumuna özgü histogenesisin migrasyon ve maturasyonun devamını gösteren bulgular saptandı. Epileptik sıçanlara ait yenidoğan cerebrum korteksinde ise daha erken embriyonal dönem cerebellar korteksine ait yapısal bulgular özellikle dendritler ve aksonal filopodia, interselüler aralık yoğunluğu perikaryonlarının çok sıralı yerleşimleri gözlemlenerek değerlendirildi. Deney gruplarına ait purkinje hücre perikaryonların iç granüler tabakanın susbtantia alba'ya bakan bölgelerinde de saptanması migrasyondaki gecikmeyi ortaya koydu. Sonuç olarak epileptik olguların hamileliklerinin kontrolü yenidoğan bakılarına olası malformasyon ve mental retardasyon yönünden incelenmesi gereklidir. İleri çalışmalarla nörogenesise etkili çinko, omega 3 gibi preparatlarla çalışmalar desteklenmeli ve ileri araştırmalar planlanmalıdır.Öğe Immunohistochemical and Histopathological Evaluation of 2,4-Dichlorophenoxyacetic Acid-Induced Changes in Rat Kidney Cortex(Springer, 2009) Uyanikgil, Yigit; Ates, Utku; Baka, Meral; Bicer, Songuel; Oztas, Ebru; Ergen, GuerselThis study aims to investigate the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on rat kidney cortex histology. Oral exposure of rats to 2,4-D for 28 days resulted in decreases in body weight gain and kidney weight. Histological examination showed degeneration in renal corpuscles and podocytes; vacuolization in the glomerulus with disintegration of the basal membrane; tissue edema; vacuolization, cystic dilation and invagination of the basal laminae in the tubular structures; dilation and congestion in renal corpuscular vessels and marked decrease in glomerular and stromal fibronectin reaction; suggesting that subacute 2,4-D administration induces dose-dependent histopathological degenerative effects in rat kidney cortex.Öğe Investigation of maternal melatonin effect on the hippocampal formation of newborn rat model of intrauterine cortical dysplasia(Springer, 2010) Baka, Meral; Uyanikgil, Yigit; Ates, Utku; Kultursay, NilgunObjectives Cortical dysplasia is a cortical malformation resulting from any developmental defects during different periods of development. This study aims to investigate the hippocampal histopathological alterations in the neonates with cortical dysplasia due to the prenatal exposure to carmustine (1,3-bis (2-chloroethyl)-1-nitrosoure; BCNU) and the possible effects of prophylaxis with melatonin, a neuroprotective agent. Methods Wistar albino female rats were randomly divided into four experimental groups; control, melatonin-treated, BCNU-exposed and BCNU-exposed+melatonin-treated. Light microscopy and immunohistochemistry were carried out on the newborn hippocampus. Results Histopathology of hippocampus from the control and melatonin-treated groups showed continuity of migration and maturation as patognomonic signs of the normal newborn hippocampus. Hippocampal cortex from the newborns exposed in utero to BCNU showed the histology of early embryonic hippocampal formation with immuno-histochemical increase in the number of nestin positive cells and decreases in the immunoreactivity of glial fibrillary acidic protein (GFAP) and synaptophysin. These findings indicate a significant delay in hippocampal maturation, migration, and synaptogenesis. Intrauterine treatment of BCNU-exposed rats with melatonin resulted in histopathological features almost similar to control group. Conclusion It has been concluded that cortical dysplasia induced by intrauterine BCNU administration results in delayed hippocampal maturation, which is successfully restored by intrauterine melatonin treatment.Öğe Light, electron microscopic and immunohistochemical study of the effect of low-dose aspirin during the proestrus phase on rat endometrium in the preimplantation period(Sci Printers & Publ Inc, 2007) Ates, Utku; Baka, Meral; Turgut, Mehmet; Uyanikgil, Yigit; Ulker, Sibel; Yilmaz, Ozlem; Tavmergen, Erol; Yurtseven, MineOBJECTIVE: To evaluate structural alterations in rat endometrium at preimplantation following treatment with aspirin beginning from proestrus by light microscopy, electron microscopy and immunohistochemical techniques. STUDY DESIGN: Twenty rats were divided into control (n =10) and experimental (n =10) groups. Experimental rats were treated with low-dose aspirin daily (2 mg/kg/day) during estrus, beginning from the proestrus phase, mated at end of cycle and treated with aspirin. Untreated pregnant rats were the control group. Rats in both groups were sacrificed at the 84th pregnancy hour; the uterus was rapidly removed and dissected free of surrounding adipose tissue. Uteri specimensfrom nonpregnant rats were transferred into fixative solution and processed for light, electron microscopic and immunohistochemical study. RESULTS: Light and electron microscopy of endometrium from control rats conformed to mid-diestrus phase; endometrial histology of the aspirin-treated group conformed to late diestrus phase. The endometrial layer was significantly thicker in the aspirin-treated group compared to the untreated control group (p < 0.001). No significant difference was found in vessel number between groups. Staining with alpha V integrin was more dense in the aspirin-treated group. CONCLUSION: Based on histologic findings, we suggest low-dose aspirin has positive effects on preparing endometrium before implantation.Öğe Maternal uygulanan valproik asit v triphenyl fosfitin yavru sıçanların ergenlik dönemlerindeki davranışsal ve hipokampal değişikliklere etkisi(Ege Üniversitesi, 2019) Baka, Meral; Bilim, PetekOtizm sosyal etkileşimdeki bozulmalar ve tekrar edici/basmakalıp davranışlar ile karakterize nörogelişimsel bir bozukluktur. Nörogelişimsel bozuklukların ortaya çıkmasında genetik ve çevresel faktörlerin etkili olduğu bilinmektedir. Otizmi etkileyen genetik faktörlerin henüz tam olarak aydınlatılamaması ile beraber çevresel faktörler üzerine yapılan araştırmalar yoğunluk kazanmış ve gebelik döneminde maruz kalınan kimyasalların doğurduğu sonuçlar oldukça önemli bir boyuta ulaşmıştır. Çalışma günlük yaşamda maruz kalınan ve nörotoksik bir kimyasal olan Trifenil Fosfit ile otizm hayvan modellemelerinde kullanılan Valproik Asit'in maternal intraperitoneal enjeksiyonunun yavru sıçanların ergenlik ve yetişkinlik dönemleri üzerindeki bilinmeyen etkilerini davranışsal ve hipokampal farklılıklar üzerinden araştırmak amacıyla planlanmıştır. Bu amaçla davranışsal testler ve histokimyasal boyama yöntemleri kullanılmıştır. Yapılan değerlendirmelerden elde edilen bilgiler erken embriyonik dönemde Trifenil Fosfit ve Valproik Asit'e maruz kalan yavruların nörogelişimsel bozukluk gösterdiklerini ortaya koymuştur. Bu yönüyle çalışma aynı zamanda gebelik döneminde çevresel kimyasallara karşı dikkatli olunması gerektiğini vurgulamaktadır.;Otizm, valproik asit, trifenil fosfit, nörogelişim, maternal, hipokampus, davranış.;Autism, valproic acid, triphenyl phosphite, neurodevelopment, maternal, hippocampus, behavior.Öğe Melatonin Attenuates Histopathological Changes in the Hippocampus of Infantile Rats with Kaolin-Induced Hydrocephalus(Karger, 2018) Turgut, Mehmet; Baka, Meral; Uyanikgil, YigitObjective/Aim: Hydrocephalus is defined as an incapacitating neurological disorder characterized by ventricular enlargement in children, but the effects of melatonin on this hydrocephalus have not yet been fully elucidated. In the present experiment, we attempted to investigate the effects of exogenous melatonin administration on hydrocephalus-induced hippocampal changes in infantile rats. Methods: In this study, we randomly divided 45 Swiss albino rats aged 2 weeks into 3 groups: group I, the control group received a sham injection with needle insertion only; groups II and III were given kaolin injections before treatment - group II, the hydrocephalus group, was treated with an isotonic NaCl solution, and group III, the hydrocephalus plus melatonin group, was treated with 0.5 mg/100 g body weight of exogenous melatonin. Both immunohistochemical and histological analyses were performed after hydrocephalus induction and melatonin administration. Immunohistochemical staining consisted antiglial fibrillary acidic protein staining. The TUNEL technique was used for defining quantitate apoptosis. Results: Melatonin administration significantly attenuated chronic hydrocephalus-induced histopathological changes in the hippocampal subregions of infantile rats. Compared to hydrocephalic rats treated with saline solution, melatonin significantly decreased the number of apoptotic cells and pyknotic index values of each hippocampal subregion after the kaolin-induced hydrocephalus (p < 0.001). Conclusion: The present results demonstrate that the chronic hydrocephalus-induced histopathological changes in the hippocampus were partially reversible with melatonin treatment, suggesting its neuroprotective effects in infantile rats. However, these findings need to be confirmed by further experimental studies and clinical trials. (C) 2018 S. Karger AG, BaselÖğe Melatonin Production and Bioavailability(Crc Press-Taylor & Francis Group, 2015) Cetin, Emel Oyku; Uyanikgil, Yigit; Turgut, Mehmet; Baka, Meral; Srinivasan, V; Gobbi, G; Shillcutt, SD; Suzen, SÖğe Neuroprotective effects of melatonin upon the offspring cerebellar cortex in the rat model of BCNU-induced cortical dysplasia(Elsevier Science Bv, 2007) Uyanikgil, Yigit; Baka, Meral; Ates, Utku; Turgut, Mehmet; Yavasoglu, Altug; Uelker, Sibel; Soezmen, Eser Yildirim; Sezer, Ebru; Elmas, Cigdem; Yurtseven, Mine ErtemCortical dysplasia is a malformation characterized by defects in proliferation, migration and maturation. This study was designed to evaluate the alterations in offspring rat cerebellum induced by maternal exposure to carmustine-[1,3-bis (2-chloroethyl)-1-nitrosoure] (BCNU) and to investigate the effects of exogenous melatonin upon cerebellar BCNU-induced cortical dysplasia, using histological and biochemical analyses. Pregnant Wistar rats were assigned to five groups: intact-control, saline-control, melatonin-treated, BCNU-exposed and BCNU-exposed plus melatonin. Rats were exposed to BCNU on embryonic day 15 and melatonin was given until delivery. Immuno/histochemistry and electron microscopy were carried out on the offspring cerebellum, and levels of malondialdehyde and superoxide dismutase were determined. Histopathologic ally, typical findings were observed in the cerebella from the control groups, but the findings consistent with early embryonic development were noted in BCNU-exposed cortical dysplasia group. There was a marked increase in the number of TUNEL positive cells and nestin positive cells in BCNU-exposed group, but a decreased immunoreactivity to glial fibrillary acidic protein, synaptophysin and transforming growth factor beta 1 was observed, indicating a delayed maturation, and melatonin significantly reversed these changes. Malondialdehyde level in BCNU-exposed group was higher than those in control groups and melatonin decreased malondialdehyde levels in BCNU group (P<0.01), while there were no significant differences in the superoxide dismutase levels between these groups. These data suggest that exposure of animals to BCNU during pregnancy leads to delayed maturation of offspring cerebellum and melatonin protects the cerebellum against the effects of BCNU. (C) 2007 Elsevier B.V. All rights reserved.Öğe Postnatal sıçan epilepsi modellerinde maternal stresin epileptojenez ve öğrenmeye etkisi(Ege Üniversitesi, 2019) Baka, Meral; Çelik, KübraStres çoğu nörolojik hastalığın tetikleyicisi olarak günlük hayatta karşımıza çıkmaktadır. Epileptik nöbetlerin de tetikleyicisi olan stres, merkezi sinir sistemi başta olmak üzere tüm vücut sistemlerini etkilemektedir. Prenatal uygulanan maternal stres ise hem embriyonik gelişime hem de postnatal gelişime etki ederek epilepsi riskini artıran bir faktör olarak karşımıza çıkmaktadır. Çalışma yeni doğanda (P10) PTZ ve hipoksi ile indüklenen modellerde nöronal göç, nörogenez ve histogenez kaynaklı hatalar nedeniyle epilepsi riskinin ergenlik döneminde epileptojenezi artırarak öğrenme ve bellek problemlerine yol açtığını göstermektedir. Embriyonik gelişimde maternal strese ve postnatal gelişimde epileptik nöbetlere maruz kalan ergen sıçanlarda gelişim geriliğiyle beraber kognitif süreçlerinin de bozulduğu ortaya konmuştur. Özellikle maternal stresin PTZ grubunu histolojik, davranışsal ve bilişsel olarak etkilemesi yavrunun gelişimi için önem arz etmektedir. Dolayısıyla geç gelişim ve epileptojenez riskinden korunmak adına gebelik döneminde stres faktörlerinden kaçınmak gerekmektedir. Hipoksinin de hipokampüste epileptik benzeri bir morfoloji sergilemesi aynı zamanda öğrenme ve bellek becerilerine olan etkileri de epileptojenez riskini artırıcı bir faktör olarak karşımıza çıkmaktadır. Fakat maternal strese sahip hipoksik grupta görülen histopatolojik ve davranışsal değişiklikler bu riski daha da artırmıştır. Prenatal stres, kontrol gruplarında öğrenme ve bellek becerilerini artırmışken bu durum PTZ ve hipoksik gruplarda tam tersi yönde etki etmiştir. Dolayısıyla çalışma, yapılacak ileriki çalışmalarda stres ve epilepsi arasındaki ilişkiye hem yapısal hem de davranışsal olarak yeni bir boyut kazandıracaktır.;Maternal stres, epilepsi, epileptojenez, öğrenme, bellek, nöronal göç, hipokampus.;Maternal stress, epilepsy, epileptogenesis, learning, memory, neuronal migration, hippocampus.Öğe Prenatal stress impairs recognition memory and leads to neurodevelopmental deficits in the hippocampus of adolescent rats with early acute pentylenetetrazole-kindling(2021) Çelik, Kübra; Bilim, Petek; Garip, Gurur; Durmaz, Burak; Sözmen, Eser Yıldırım; Baka, MeralObjectives: This study aimed to investigate the effects of prenatal stress (PS) on hippocampus of early acute pentylenetetrazole (PTZ)-kindled offspring in adolescence. Recognition memory, morphological changes and synaptophysin levels in hippocampus were evaluated. Methods: Restraint stress was induced to a group of pregnant dams and non-stressed (NA) group remained undisturbed. Next, male and female offspring were divided as 1. PS-PTZ, 2. PS -control, 3. NA-PTZ and 4. NA-control (n = 12 in each group). The object recognition test was performed following PTZ injection (45 mg/kg) on postnatal day 10 (P10). Brains were collected on postnatal day 35 (P35) to determine neuronal density and synaptophysin expression by immuno/-histological studies. Further, oxidative stress products in hippocampus were analyzed with different biochemical assays. Results: PS impaired recognition memory in PTZ group significantly (p = 0.03); however, the impairment of PS was reversible in control group compared to PTZ (p = 0.04). Furthermore, PS caused neuronal loss in CA1 (p = 0.01) and decreased synaptophysin expression in the CA3 area of hippocampus in PTZ group (p = 0.03). PS also increased the oxidative stress markers in PTZ group significantly (p < 0.05). Conclusions: These results suggest that PS causes neurodevelopmental deficits in adolescent hippocampus and recognition memory after early-life seizures prominently. However, the damage of only PS in adolescence can be reversible. Therefore, the effects of PS in the adult hippocampus and other regions of brain need to be further studiedÖğe Rattus albinuslara uygulanan subdiaphragmatic truncal bilateral vagotomynin karaciğerin glikojeni ve yapısı üzerine etkisi(Ege Üniversitesi, 1991) Baka, MeralÖZET Çalışmamız Rattus Albinuslarda O kontrol 24 tfago- tomilri vaka üzerinde gerçekleştirildi «Bilateral Sub- diafragmatik vagotomi uygulanan Rattus albinus karaciğerleri PAS ve DBPAS ile boyanıp ışık mikroskobun da incelendi. Bölgesel ve hücresel glikojen yoğunluklarında bü yük bir azalma olduğu saptandı. Hepatoeytlerin gliko jen içerikleri A-, başlangıcında yoğun, özellikle A« de oldukça azalmıştır. Glikojen içeren hücrelerde glikojenin granüler yapısı yüksek oranda granüler olmak yerine adacıklar tarzında bulunmuştur. Sinuzoidlerde aşırı genişlemenin yanısıra vena centralis ve vena interlobularislerdede genişleme görülmüş tür. liu genişleme venoz s tazı destekler ölçü de morfolojik değişiklikler hepatoeytlerin poligonal şeklinin kaybıyla başlar. Hücrelerin s t oplazma, nucleus metrik oranlarının azaldığı gözlenmiştirÖğe Rattus albinuslarda subdiaphragmatic Truncal bilateral vagotomy'den sonra midenin değişik bölümlerindeki myelinli halkaların dağılımı(Ege Üniversitesi, 1988) Baka, MeralÖZET Çalışmamız Rattus Albinuslarda 8 kontrol 34- VAGOTO- MÎLÎ vak' a üzerinde gerçekleştirildi. Kesitler Weigert Landau myelin boyası ile boyanıp bulgular ışık mikrosko- bi düzeyinde incelendi. Midenin innervasyonuna katılan sinirlerde ve vagusun dev re dışı bırakılması ile kalan sinirlere ait myelinli ipliklerin kalitatif ve kantitatif incelenmesi yapıldı. Alınan sonuçlara göre : Yagus yapısındaki myelinli lifler 0,5 - 5 M çapta ve 0,25 - l»5>u kalınlıktadır. Truncus sympathicusda. 0,5 - 1»5ju çap 0,25 - 0,5 ai kalınlık N.phrenicusda ise 4-6/ı çap ve 1 m kalınlıkta bulundu. Vagotomili vak' alarda 2 ai ve dana az çapta olanlar intakt kaldı. Oesophagus dorsal dış musculer tabakada lı5 - 3 M çap 0,5/ı kalınlıkta neuritler bulundu. Mide serozasmda 1 - 2 ju. çap ve 0,25 - 0,5 ai kalınlıkta neuritlerle kaplı sinir kesitleri, ikinci olarak da 0,5. l,5/ı çap ve 0,25 - 0,5 m kalınlığa sahip neuritleri az sayıda bulunduran sinir kesitleri tesbit edildi. I.gurup- takiler vagotomili vak1 alarda azaldı. Mide tunica musculariste 0,5 ai ve daha az çaplı mucozada 1 Ai 0,5-*ı ve daha az çaplı fibriller tesbit edildi. Genel olarak vagotomili vak' alarda neuritlerin kol- labe olduğu izlendi
