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Öğe Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent(Elsevier, 2015) Kalburcu, Tulden; Tabak, Ahmet; Ozturk, Nevra; Tuzmen, Nalan; Akgol, Sinan; Caglar, Bulent; Denizli, AdilThis study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.Öğe Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent(Elsevier, 2015) Kalburcu, Tulden; Tabak, Ahmet; Ozturk, Nevra; Tuzmen, Nalan; Akgol, Sinan; Caglar, Bulent; Denizli, AdilThis study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.Öğe Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent(Elsevier, 2015) Kalburcu, Tulden; Tabak, Ahmet; Ozturk, Nevra; Tuzmen, Nalan; Akgol, Sinan; Caglar, Bulent; Denizli, AdilThis study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.Öğe Affinity-Based Magnetic Nanoparticle Development for Cancer Stem Cell Isolation(Mdpi, 2024) Kuru, Cansu Ilke; Ulucan-Karnak, Fulden; Dayioglu, Buesra; Sahinler, Mert; Sendemir, Aylin; Akgol, SinanCancer is still the leading cause of death in the world despite the developing research and treatment opportunities. Failure of these treatments is generally associated with cancer stem cells (CSCs), which cause metastasis and are defined by their resistance to radio- and chemotherapy. Although known stem cell isolation methods are not sufficient for CSC isolation, they also bring a burden in terms of cost. The aim of this study is to develop a high-efficiency, low-cost, specific method for cancer stem cell isolation with magnetic functional nanoparticles. This study, unlike the stem cell isolation techniques (MACS, FACS) used today, was aimed to isolate cancer stem cells (separation of CD133+ cells) with nanoparticles with specific affinity and modification properties. For this purpose, affinity-based magnetic nanoparticles were synthesized and characterized by providing surface activity and chemical reactivity, as well as making surface modifications necessary for both lectin affinity and metal affinity interactions. In the other part of the study, synthesized and characterized functional polymeric magnetic nanoparticles were used for the isolation of CSC from the human osteosarcoma cancer cell line (SAOS-2) with a cancer stem cell subpopulation bearing the CD133 surface marker. The success and efficiency of separation after stem cell isolation were evaluated via the MACS and FACS methods. As a result, when the His-graft-mg-p(HEMA) nanoparticle was used at a concentration of 0.1 mu g/mL for 106 and 108 cells, superior separation efficiency to commercial microbeads was obtained.Öğe Antibody separation using lectin modified poly(HEMA-EDMA) hydrogel membranes(Taylor & Francis Ltd, 2018) Demir, Esra Feyzioglu; Kuru, Cansu Ilke; Uygun, Murat; Uygun, Deniz Aktas; Akgol, SinanHerein we describe the synthesis of Concanavalin A-poly(2-hydroxyethyl methacrylate-ethylene dimethacrylate) hydrogel membranes (via photopolymerization technique) for antibody separation from aqueous solutions. Different characterization techniques including Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, Elemental Analysis and swelling tests revealed the highly rough morphology and spherical shape of the synthetized membranes. Attached amount of IMEO (salinization agent) onto polymeric structure and Con A binding capacity were found to be 10.85 mol/g and 3.52 mg/g, respectively. Optimum conditions for IgG adsorption such as adsorption capacity, pH and reusability profile of HMs were judiciously characterized. Maximum IgG adsorption capacity of hydrogel membrane was found to be as 26.81 mg/g. Adsorbed IgG was eluted successfully by using 2.0 M of NaCl solution. Reusability profiles of hydrogel membrane in five adsorption-desorption cycles revealed that there was no significant decrease in IgG adsorption capacity at the end of the 5th reuse. The hydrogel membranes reported here hold considerable promise as an effective sorbent system for IgG adsorption with good stability and efficient repeated usage.Öğe Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin(Humana Press Inc, 2010) Avcibasi, Nesibe; Uygun, Murat; Corman, M. Emin; Akgol, Sinan; Denizli, AdilSupermacroporous poly{2-hydroxyethyl methacrylate-co-[N,N-bis(2,6-diisopropylphenyl)-perylene-3,4,9,10-tetracarboxylic diimide]} [poly(HEMA-co-DIPPER)] monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with DIPPER as functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 mu m size. Poly(HEMA-co-DIPPER) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly (HEMA-co-DIPPER) cryogel was 14.7 g H2O/g dry cryogel. Poly(HEMA-co-DIPPER) cryogel was used in the adsorption/desorption of albumin from aqueous solutions. The nonspecific adsorption of albumin onto plain poly(HEMA) cryogel was very low (3.36 g/g polymer). The maximum amount of albumin adsorption from aqueous solution in acetate buffer was 40.9 mg/g polymer at pH 5.0. It was observed that albumin could be repeatedly adsorbed and desorbed with the poly(HEMA-co-DIPPER) cryogel without significant loss of adsorption capacity.Öğe Boronate affinity nanoparticles for nucleoside separation(Taylor & Francis Ltd, 2016) Okutucu, Burcu; Vurmaz, Deniz; Tuncal, Akile; Turkcan, Ceren; Uygun, Deniz Aktas; Akgol, SinanBoronate affinity systems have been recently used for the specific isolation of cis-diol group carrying biomolecules such as glycoproteins, nucleosides, carbohydrates. Nanosized materials have been extremely used for the biotechnological purposes due to their unique properties and their high surface areas. The objective of this presented work was to develop a new boronate affinity system for the nucleoside adsorption. For this purpose, poly(HEMA) nanoparticles were synthesized by using surfactant free emulsion polymerization technique and then functionalized with phenylboronic acid. Synthesized nanoparticles were characterized with FTIR, SEM, and Zeta size analysis. Nucleic acid adsorption experiments were repeated for different medium pH values, for various nucleosides concentrations, for different temperatures and ionic strengths, in order to determine the optimum adsorption conditions. In the light of these studies, it can be concluded that this boronate ligand carrying nanoparticles were very valuable for the separation of nucleosides.Öğe Boronate affinity nanoparticles for RNA isolation(Elsevier Science Bv, 2015) Toprak, Aykut; Gorgun, Cansu; Kuru, Cansu Ilke; Turkcan, Ceren; Uygun, Murat; Akgol, SinanIn this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.Öğe Boronate affinity nanoparticles for RNA isolation(Elsevier Science Bv, 2015) Toprak, Aykut; Gorgun, Cansu; Kuru, Cansu Ilke; Turkcan, Ceren; Uygun, Murat; Akgol, SinanIn this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.Öğe Boronate affinity nanoparticles for RNA isolation(Elsevier Science Bv, 2015) Toprak, Aykut; Gorgun, Cansu; Kuru, Cansu Ilke; Turkcan, Ceren; Uygun, Murat; Akgol, SinanIn this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.Öğe Cholesterol removal onto the different hydrophobic nanospheres: A comparison study(Elsevier Science Inc, 2014) Kalburcu, Tulden; Ozturk, Nevra; Tuzmen, Nalan; Akgol, Sinan; Denizli, AdilThe aim of this study is to prepare two different hydrophobic polymeric nanospheres for cholesterol removal. Poly(HEMA-MAT) nanospheres with an average size of 100 nm and poly(HEMA-MAP) nanospheres with an average size of 158 nm were produced by surfactant free emulsion polymerization of HEMA and MAT and MAP monomers. These hydrophobic nanospheres were characterized by FTIR, SEM, elemental analysis, particle size and surface area measurements. Cholesterol removal experiments were performed in a batch experimental set-up and removal medium was methanol. Cholesterol adsorption capacity of poly(HEMA-MAP) nanospheres was approximately three times higher than that of poly(HEMA-MAT) nanospheres. (C) 2013 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.Öğe Commercial roadmap of nanobiosensor development(Frontiers Media Sa, 2024) Ulucan-Karnak, Fulden; Kuru, Cansu Ilke; Akgol, SinanA nanobiosensor is a tool that converts a biological stimulus into an electrical output via nanosized transducer elements. Nanobiosensors are promising instruments, especially in biomedical applications in the literature and industry. To develop a nanobiosensor from idea to product, a life-cycle approach that comprises various processes ranging from conception through commercialization is required. Developers and potential investors should examine market requirements, design possibilities, feasibility, financial return, and risk assessments when developing a nanobiosensor development concept. It is critical to establish a well-defined regulatory pathway for bringing innovation to market at a low cost and in a short period. R&D should conduct thorough examinations of nanomaterial toxicity and health effects, involving marketing, advertising, and financial analysis. Stakeholders should discuss technology transfer office protocols for faster, healthier operations.Öğe Concanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilization(Elsevier Science Bv, 2012) Uygun, Murat; Uygun, Deniz Aktas; Ozcaliskan, Emir; Akgol, Sinan; Denizli, AdilConcanavalin A (Con A) immobilized supermacroporous poly(ethylene glycol dimethacrylate) [poly(EGDMA)] monolithic cryogel column was prepared by radical cryocopolymerization of EGDMA as a monomer and N,N'-methylene-bisacrylamide as a crosslinker. Bioligand Con A was then immobilized by covalent binding onto poly(EGDMA) cryogel via glutaraldehyde activation [Con A-poly(EGDMA)]. Con A-poly(EGDMA) cryogel was characterized by swelling studies and scanning electron microscopy. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 mu m size. The equilibrium swelling degree of the cryogel was 15.01 g H2O/g dry cryogel. Con A-poly(EGDMA) cryogel was used in the adsorption/desorption of invertase from aqueous solutions. The maximum amount of invertase adsorption from aqueous solution in acetate buffer was 55.45 mg/g polymer at pH 5.0. Con A-poly(EGDMA) cryogels were used for repetitive adsorption/desorption of invertase without noticeable loss in invertase adsorption capacity after 10 cycles. (c) 2012 Elsevier By. All rights reserved.Öğe Conformational and electronic properties of N-methacryloyl-(L)-glutamic acid(Taylor & Francis Ltd, 2017) Parlak, Cemal; Kesan, Gurkan; Kazanci, Nadide; Rhyman, Lydia; Ramasami, Ponnadurai; Alver, Ozgur; Akgol, SinanConformers of N-methacryloyl-(L)-glutamic acid (MAGA, C9H13NO5) in the gas phase, benzene or methanol environment were searched by the density functional theory (DFT) and time-dependent DFT. Selected conformational isomers based on potential energy surface analysis were studied by considering the structural and electronic properties. B3LYP functional, and HF and MP2 levels were used with the 6-31+G(d,p), 6-311+G(3df,p) and aug-cc-pVDZ basis sets. We explored the effect of medium on the conformational preferences, UV spectra and frontier molecular orbitals of the conformers. The outcomes of this research will be useful for future studies including moieties analogous to MAGA.Öğe Controlled release of curcumin from poly(HEMA-MAPA) membrane(Taylor & Francis Ltd, 2017) Caka, Muserref; Turkcan, Ceren; Uygun, Deniz Aktas; Uygun, Murat; Akgol, Sinan; Denizli, AdilIn this work, poly(HEMA-MAPA) membranes were prepared by UV-polymerization technique. These membranes were characterized by SEM, FTIR, and swelling studies. Synthesized membranes had high porous structure. These membranes were used for controlled release of curcumin which is already used as folk remedy and used as drug for some certain diseases and cancers. Curcumin release was investigated for various pHs and temperatures. Optimum drug release yield was found to be as 70% at pH 7.4 and 37 degrees C within 2 h period. Time-depended release of curcumin was also investigated and its slow release from the membrane demonstrated within 48 h.Öğe Development of amino functionalized carbon coated magnetic nanoparticles and their application to electrochemical detection of hybridization of nucleic acids(Elsevier Science Bv, 2017) Altay, Cansu; Senay, R. Hilal; Eksin, Ece; Congur, Gulsah; Erdem, Arzum; Akgol, SinanIn our study, the development of amino functionalized carbon coated magnetic nanoparticles (NH2-CC-MNPS) and their usage for electrochemical detection of hybridization of nucleic acids have been aimed. Firstly, NH2CC-MNPS were prepared by coating of pristine Fe3O4 nanoparticles with two layers via caramelization and silanization processes respectively. After the morphological characterization with scanning electron microscopy (SEM) it was seen that NH2-CC-MNPs was spherical shaped and in 28 nm sized. Investigation of chemical composition with the help of scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDX) and fourier transform infrared spectroscopy (FTIR) was showed incorporation of carbon and APTES to the structure of NH2-CC-MNPs. Magnetic property of NH2-CC-MNPs after two layered coatings was demonstrated with electron spin resonance (ESR) technique and gfactor was calculated as 2.6. In the second part of this study, optimization studies have carried out onto the surface of NH2-CC-MNPS prepared in saltless phosphate-tween 20 buffer (PBTw) for the analysis of DNA hybridization. The thiol linked DNA probe sequence representing to the Hepatitis B virus (HBV) concentration, target DNA sequence concentration, the most productive hybridization time and the selection of the nanoparticle surfaces have been researched. The electrochemical detection of DNA hybridization was investigated using PGE in combination with differential pulse voltammetry (DPV) technique by measuring the guanine oxidation signal. The detection limit was calculated in the linear target DNA concentration range of 5-25 rho/mL and it was found to be 1.15 mu g/mL (20 pmol in 110 mu L solution). It has been intended to be more reproducible, more sensitive and faster results with developed biosensor technology.Öğe Development of functionalized polymeric nanomaterial by organosiliconic reagent for boronate affinity-based dopamine recognition(Elsevier Science Sa, 2024) Ulucan-Karnak, Fulden; Kuru, Cansu Ilke; Akgol, SinanThe dopamine neurotransmitter is mainly responsible for endocrine functions in our body. The increase in the level of dopamine in the human body leads to many disorders such as schizophrenia, uncontrollable tics, addiction, increased tension, desire to act excessively, and carelessness. Decreased levels of dopamine cause problems such as malnutrition, stress, insomnia, and the use of antidepressants. Recognition of dopamine is crucial for clinical experiments. Therefore, the development of recognition surfaces of dopamine is essential for isolation, purification, and determination processes. This study, it was aimed to produce a polymeric nanomaterial that can recognize dopamine. For this purpose, p(HEMA) polymer was synthesized with surfactant-free emulsion polymerization method and silanized by organosilicon reagent (3-aminopropyl) triethoxylsilane (APTES) and modified with phenylboronic acid (PBA). Characterization of the p(HEMA)-APTES-PBA nanopolymeric system for dopamine recognition was performed with Scanning Electron Microscope(SEM), Energy Dispersive Spectrometry (EDS), Fourier Transform Infrared Spectroscopy (FTIR), Zeta-size/zeta-potential analysis, surface area calculations. Dopamine affinity of the p(HEMA)-APTES-PBA was optimized in terms of pH, time, concentration, buffer concentration, and buffer type parameters. Dopamine adsorption of p(HEMA)-APTESPBA nanopolymers was 4,8 times more than epinephrine in specificity analysis. After the 7 adsorption-desorption cycles, the desorption rate was found to be 74.8 %. The developed nanopolymeric system is a convenient method with high adsorption capacity which allows easy and rapid extraction, isolation, and purification of dopamine.Öğe Development of nanobiosensor for therapeutic drug monitoring in personalized cancer treatment approach(Taylor & Francis Ltd, 2024) Kuru, Cansu Ilke; Sipahi, Deniz; Aydogan, Ceren; Ulucan-Karnak, Fulden; Akgol, SinanDocetaxel is one of the most effective and safe chemotherapy drugs according to the World Health Organization, but its clinical use has been discontinued due to its various side effects. To reduce these side effects, the amount of docetaxel drug should be kept at the most effective level, it should be monitored in body fluids. Due to the limitations of traditional analytical methods used for this purpose, such as expensive and low sensitivity, labor-intensive and time-consuming complex preliminary preparation, efficient methods are required for the determination of the docetaxel level in the body. The increasing demand for the development of personalized therapy has recently spurred significant research into biosensors for the detection of drugs and other chemical compounds. In this study, an electrochemical-based portable nanobiosensor system was developed for the rapid, low-cost, and sensitive determination of docetaxel. In this context, mg-p(HEMA)-IMEO nanoparticles to be used as nanobiosensor bioactive layer was synthesized, characterized, and docetaxel determination conditions were optimized. According to the results obtained, the developed nanobiosensor system can detect docetaxel with a sensitivity of 2.22 mg/mL in a wide calibration range of 0.25-10 mg/mL, in only 15 min, in mixed media such as commercially available artificial blood serum and urine. determined. We concluded that the developed nanobiosensor system can be successfully used in routine drug monitoring as a low-cost biomedical device capable of direct, rapid, and specific drug determination within the scope of personalized treatment, providing point-of-care testing. Therapeutic drug monitoring on-site has the potential to significantly save healthcare expenditures while also improving patient outcomes.Chromatography's applicability as a routine procedure is restricted by its lack of standardization, expensive equipment, lengthy turnaround times, and labor-intensive sample preparation.Overcoming these drawbacks, nanobiosensors provide an inexpensive, user-friendly, on-site analytical approach to fully explore the possibilities of therapeutic drug monitoring. In the graphical abstract, the nanobiosensor system for the docetaxel anticancer drug developed within the scope of the study is schematized. Nanoparticles with developed magnetic properties and modified specifically for docetaxel are used as a bioactive layer on the surface of the disposable surface imprinted electrode, and blood or urine samples taken from patients provide the amount of docetaxel in their content with potentiostat measurements, through this bioactive layer.Öğe DNA isolation by galactoacrylate-based nano-poly(HEMA-co-Gal-OPA) nanopolymers(Taylor & Francis Ltd, 2017) Kayhan, Ceren Turkcan; Ural, Fulden Zeynep; Koruyucu, Meryem; Salman, Yesim Gul; Uygun, Murat; Uygun, Deniz Aktas; Akgol, Sinan; Denizli, AdilIsolation of DNA is one of the important processes for biotechnological applications such as investigation of DNA structures and functions, recombinant DNA preparations, identification of genetic factors and diagnosis and treatment of genetic disorders. The aim of this study was to synthesis and characterizes the galactoacrylate based nanopolymers with high surface area and to investigate the usability of these synthesized nanopolymers for DNA isolation studies. Nanopolymers were synthesized by the surfactant free emulsion polymerization technique by using the monomers of 2-hydroxyl ethylmethacrylate and 6-O-(2'-hydroxy-3'-acryloyloxypropyl)-1,2:3,4-di-O-isopropylidene-alpha-D-galactopyranose. Galactoacrylate origin of these newly synthesized nanopolymers increased the interaction between DNA and nanopolymers. Prepared nanopolymers were characterized by SEM, FT-IR and ZETA sizer analysis. Synthesized nanopolymers were spherical, and their average particle size was about 246.8nm. Adsorption of DNA onto galactoacrylate based nanopolymers was investigated by using different pHs, temperatures, ionic strength, DNA concentrations and desorption studies and maximum DNA adsorption was found to be as 567.12 mg/g polymer at 25 degrees C, in pH 5.0 acetate buffer. Reusability was investigated for 5 successive reuse and DNA adsorption capacity decreased only about 10% at the end of the 5th reuse.Öğe Dye Attached Nanoparticles for Lysozyme Adsorption(Taylor & Francis Inc, 2014) Uygun, Murat; Uygun, Deniz Aktas; Altunbas, Canan; Akgol, Sinan; Denizli, AdilIn this work, Reactive Blue 15 dye functionalized poly(HEMA) nanoparticles were synthesized for reversible adsorption of lysozyme from its aqueous solution. For this, nano-sized poly(HEMA) nanoparticles were synthesized by the surfactant free emulsion polymerization. Reactive Blue 15 dye then covalently attached to the polymeric structure. These novel dye attached poly(HEMA) nanoparticles were used for the adsorption of lysozyme. Characterization of dye attached nanoparticles was carried out by using FTIR, AFM, and elemental analysis. Incorporation of the dye onto the polymeric structure was demonstrated by FTIR and elemental analysis, while the size and the shape of the nanoparticles were shown by AFM. The incorporated amount of the dye was found to be 70.3 mu mol/g nanoparticle with sulphur stoichiometry and it was found that the prepared nanoparticles were in a spherical form and were about 100nm diameter. Lysozyme adsorption studies were carried out with different conditions (pH, lysozyme concentration, temperature, and ionic strength) and maximum lysozyme adsorption was found to be 630.7mg/g nanoparticle at pH 7.0 in 25 degrees C medium temperature. Adsorbed lysozyme was desorbed by 1.0M of NaCl with 96% recovery and synthesized dye-attached nanoparticles were used 10 times without any decrease in their adsorption capacity.
