Yazar "Özkalay N." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Comparison of the proportion dilution method and E-test for ofloxacin and ciprofloxacin susceptibility testing of mycobacterium tuberculosis [Mycobacterium tuberculosis suşlarinda ofloksasi·n ve si·profloksasi·n duyarliliginin saptanmasinda standart oranti di·lüsyon i·le E-test yöntemleri·ni·n karşilaştirilmasi]
    (2001) Çavuşoglu C.; Çiçek Saydam C.; Burhanoglu D.; Özkalay N.; Özkaya D.; Özçam H.; Bilgiç A.
    In this study, the antibacterial activities of ofloxacin and ciprofloxacin on 100 clinical isolates of Mycobacterium tuberculosis were investigated by using standard proportion dilution method and E-test. The sensitivities of the isolates to conventional antibiotics (isoniazide, ethambutol, rifampin, streptomycin) were determined by Bactec system and 6 were found resistant to isoniazide, while 4 strains showed multi-drug resistance. All of the 10 resistant strains and 88 of 90 sensitive strains (98%) were found susceptible to ofloxacin and ciprofloxacin by both proportion dilution method and E-test. Two of the isolates (2%) which were susceptible to first choise antituberculous drugs, were found resistant to ofloxacin and ciprofloxacin by both of the methods. As a result, the accordance between E-test and standard proportion dilution method was detected as 100 percent.
  • Küçük Resim Yok
    Öğe
    Investigation of human herpesvirus 6 infections in kidney and bone marrow/stem cell transplant recipients [Böbrek ve kemi·k i·li·gi·/kök hücre alicilarinda i·nsan herpesvi·rus 6 enfeksi·yonunun araştirilmasi]
    (2003) Özkalay N.; Özacar T.; Aşçi G.; Töz H.; Kansoy S.
    In this study, active human herpesvirus (HHV)-6 infection were investigated in 39 renal and 9 bone marrow/stem cell transplant recipients. For this purpose, the presence of HHV-6 DNA in patients sera have been searched by nested polymerase chain reaction (nPCR). In addition, HHV-6 IgM and IgG antibodies were performed by micro-enzyme immunoassay (EIA) to detect seronegative patients before transplantation and IgM response in active or primary HHV-6 infection. Active infection with HHV-6 DNA positivity was detected in 5.3% of renal and 22.2% of bone marrow/stem cell transplant recipients. Active HHV-6 infection was found to be related with asemptomatic reactivation, graft disfunction and cytomegalovirus disease in renal transplant recipients, and, fever and graft versus host disease in bone marrow/stem cell transplant recipients. It has been concluded that, the investigation of HHV-6 DNA by nPCR in the transplant sera, was a practical and useful method for the laboratories, in order to diagnose active HHV-6 infection, while HHV-6 IgG antibody detection was also useful for the differentional diagnosis of primary infection or reactivation/reinfection, but HHV-6 IgM antibodies has low value to detect active HHV-6 infection.
  • Küçük Resim Yok
    Öğe
    Susceptibility of Mycobacterium tuberculosis strains to first-line and second-line antituberculosis drugs in Ege University Hospital
    (2001) Çiçek Saydam C.; Çavuşoglu C.; Burhanoglu D.; Özkalay N.; Badak F.Z.; Bilgiç A.
    Recently tuberculosis has shown a speedy worldwide spread. The incidence of drug-resistant Mycobacterium tuberculosis is increasing in almost all industrialized and developing countries. The epidemiology of multiple drug resistance varies in different regions and countries. The aim of this study was to determine the activities of first-line (isoniazid, rifampin, ethambutol and streptomycin) and second-line (kanamycin, para-aminosalicylic acid, ethionamide and capreomycin) antituberculosis drugs on 100 various clinical isolates of M. tuberculosis. Mycobacterium tuberculosis ATCC 27294, ATCC 35838, ATCC 35825 and ATCC 35837 were used for internal quality control. First-line drug resistant strains were isolated from 10 clinical specimens. Six of them showed resistance to a single drug and four to more than one first-line drug. All of the single-drug resistant strains were resistant to isoniazid. Of 100 isolates, 56 were resistant to capreomycin, 41 to kanamycin, 12 to para-aminosalicylic acid and four to ethionamide. All of the first-line drug-resistant strains were found to be susceptible to para-aminosalicylic acid and ethionamide. In view of the above findings, we suggest that clinicians should be well-informed about the current local epidemiology of tuberculosis, and health care institutions should maintain up-to-date drug susceptibility data on the local isolates of M. tuberculosis.