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Öğe The effects of preconditioning with IFN-?, IL-4, and IL-10 oncostimulatory ligand expressions of mesenchymal stem cells(2021) Özdemir, Alper Tunga; Özdemir, Rabia Bilge Özgül; Çakır, Büşra; Darıverenli, Ertan; Kırmaz, Cengiz; Özbilgin, Kemal; Öztatlıcı, MustafaObjectives: Mesenchymal stem cells (MSCs) are strong immunomodulatory cells, and co-stimulation may play an im portant role in increasing the effects of MSCs on adaptive immune cells. Preconditioning may add to the effectivenessof MSCs. The aim of this study was to investigate alterations in the costimulatory ligand expressions of MSCs precondi tioned with inflammatory cytokines. Methods: MSCs were preconditioned with interferon gamma (IFN-?), interleukin (IL) 4 (IL-4), and IL-10, and changes inCD80, CD86, CD137L, CD252, CD274, CD275, and human leukocyte antigen (HLA) class I and II expressions were ana lyzed using flow cytometry and quantitative polymerase chain reaction methods. Human acute monocytic leukemiacell line (THP-1) macrophages preconditioned under the same conditions served as a control for comparison. Results: The frequencies of CD80 (p=0.0003), CD86 (p<0.0001), CD137L (p<0.0001), CD252 (p=0.0003), CD274(p=0.0077), CD275 (p<0.0001), and HLA-II (p<0.0001) -positive MSCs was significantly lower than that of the THP-1macrophages with either method, but there was no significant difference in the HLA-I (p=0.1506) cells. Comparison ofthe expression of the costimulatory ligands revealed that the expression of MSCs was significantly lower than that ofTHP-1 cells, and was not affected by cytokine stimuli. Conclusion: The study data indicated that although MSCs are strong immunomodulatory cells, the costimulatory li gand expression required for an effective antigen presentation was extremely low compared with that of professionalantigen presenting cells. In addition, preconditioning with IFN-?, IL-4, and IL-10 failed to increase the expression ofimportant costimulatory ligands, such as CD80 and CD86, in MSCs. The stability of costimulatory ligand expressionsuggests that MSCs may be an effective source for HLA-I-mediated peripheral tolerance.Öğe Evaluation of JAK/STAT Signaling Pathway-associated Protein Expression at Implantation Period: An Immunohistochemical Study in Rats(2022) Köse, Can; Erışık, Derya; Vatansever, H. Seda; Özbilgin, Kemal; İnan, Sevinç; Kırmaz, Cengiz; Gürel, ÇevikObjective: The implantation period of gestation is a complex process in which numerous molecular pathways play a role. The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway is one of the evolutionarily conserved cascades used to transduce a multitude signals for several biological events such as implantation and uterine receptivity. Previous studies have indicated that the implantation process is disrupted by the lack of proteins involved in this pathway. However, our literature knowledge showed that there is no study evaluated the expression of JAK/STAT signaling pathwayassociated proteins during the implantation period. This study investigated the expression patterns of JAK/STAT signaling pathway-associated proteins in rats by immunohistochemical (IHC) staining according to gestational days. Methods: In this study, thirty Wistar Albino rats weighing 250-300 g, without any problems in their menstrual cycles, were used. The pregnant animals were sacrificed on the 4th, 5th, and 6th days and histochemical and IHC analysis were performed on the uterine tissues taken from these animals. Results: In this study, protein expression of JAK1, JAK2, JAK3, STAT2, STAT3, STAT4, STAT5, and STAT6 belonging to the JAK/STAT pathway was evaluated in the uterine surface epithelium, gland structures, antimesometrial region, cells of the immune system, myometrium, mesometrial region and decidual cells, which are associated with the implantation process. The result of this evaluation reveals that the expression levels of these proteins in the JAK/STAT pathway vary in different days of the implantation period in implantation-related structures. Conclusion: This study indicates that JAK/STAT signaling pathway-associated proteins can function actively in the regulation of the immunological response of the uterus and embryo-uterus interaction during the implantation period in rats. However, the findings obtained from advanced research on JAK/STAT pathway can be used for treating recurrent pregnancy failures and in enhancing assisted reproductive technology.Öğe Kısa süreli yüksek doz büyüme hormonu uygulamasının sıçan kalbinde oluşturduğu değişikliklerin incelenmesi(2001) Özbilgin, Kemal; Coşkun, Ş; Vatansever, Seda; Ersoy, B; Tuğlu, İbrahim; Uysal, Ayşegül; Önağ, A…Öğe Maternal thyrotrophin releasing hormone TRH administration accelerates maturation in fetal rat lung A morphologic study by electron microscopy(2000) Vatansever, Seda; Laçin, S; Özbilgin, Kemal; Uysal, Ayşegül; Çağlar, H; Koyuncu, F; Yurtseven, Mine…Öğe Sıçan İmplantasyon Dönemi Boyunca Uterusların Histolojik Değerlendirmesi(2015) Ekizceli, Gülçin; İnan, Sevinç; Öktem, Gülperi; Onur, Ece; Özbilgin, Kemalİnsanlarda embriyo implantasyon süreci karmaşık fizyolojik mekanizmalar içermekte olup, sağlıklı gebeliğin elde edilmesi için fertilizasyonun ardından gerçekleşen en önemli aşamadır. İnsanlarda fertilizasyonun ardından 6-10. günler, insanlara benzer döngüleri nedeni ile model organizma olarak kullanılan sıçanlarda ise 4.5-6.5. günler "implantasyon penceresi" adı verilen süreci kapsamaktadır. İnfertiliteye neden olan mekanizmaların araştırıldığı çalışmalarda implantasyon süreci önemli rol oynamaktadır. Bu çalışmada sıçan implantasyon dönemi ile ilgili histolojik değerlendirmeler ve elde edilen son literatür bilgilerinin derlenmesi ile yeni çalışmalar için kaynak oluşturulması amaçlanmıştır. Vaginal yayma ile östrus evresinde belirlenen dişi sıçanlar, fertilizasyonun ardından; embriyonik gelişimin 4.5, 5.5. ve 6.5. günü olmak üzere 3 gruba ayrıldı ve grupları belirlenen dişi sıçanlardan (n:21) uterus örnekleri elde edildi. Dokular %10'luk formalinde tespit edilerek, rutin parafin doku takibi ile parafine gömüldü. Beş µm.'lik parafin kesitler alınarak Hematoksilen-Eozin ile boyandı ve Periyodik asit Shiff reaksiyonu ile değerlendirildi. Boyanmış kesitlerde implantasyon sürecinde uterustaki temel yapılar değerlendirildi. Kesitlerde implantasyon sürecinde görülen temel yapılar olan endometriyum, miyometriyum, perimetriyum, yüzey epiteli, primer ve sekonder desidual alanlar, GMB hücreleri, trofoblast ve embriyoblast hücreleri gösterilmiştir. Sonuçlar implantasyon dönemi evreleri boyunca karşılaştırılmıştır. Sağlıklı gebeliğin elde edilmesi için en önemli evrelerden biri olan implantasyon süreci model organizma olarak yaygın olarak kullanılan sıçanlarda evrelere göre değerlendirilmiştir.
