Development of molecularly imprinted Acrylamide-Acrylamido phenylboronic acid copolymer microbeads for selective glycosaminoglycan separation in children urine

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dc.contributor.authorUygun, Zihni Onur
dc.contributor.authorOkutucu, Burcu
dc.contributor.authorHacikara, Sukriye
dc.contributor.authorSagin, Ferhan
dc.date.accessioned2021-05-03T20:38:41Z
dc.date.available2021-05-03T20:38:41Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.descriptionUygun, Zihni Onur/0000-0001-9045-7271; okutucu, burcu/0000-0002-0907-4175en_US
dc.description.abstractBackground: in this study, we synthesized molecularly imprinted copolymers for liquid chromatography columns as a separator for glycosaminoglycan (dermatan sulfate; DS and chondroitin sulfate; CS) in urine. Materials and methods: Acrylamide and acrylamido phenylboronic acid were used as monomers, acrylamide was used for as base monomer to attract negatively charged groups and acrylamido phenylboronic acid (AAPBA) residues used to form diol bonds between sugar and boronic acid residues to strengthen the attraction. These monomers were synthesized by using precipitation polymerization to form uniform spheres, which are more durable for the pressurized chromatographic systems. Trimethylolpropane trimethacrylate and AIBN were used as crosslinker and starter, respectively. Results: These GAG selective polymers were filled by pressurized flow into the steel (4.6 mm x1.6 mm) columns, then imprinted GAGs were extracted and analyzed to calculate binding capacity of each milligram polymer. Calibration curves of the GAG selective columns were obtained 62.5 -1000 ng/mL less than 5% coefficient variation, and lower matrix effect. Conclusion: Our imprinted columns separated different GAGs from urine specifically and sensitively. Matrix effect was at an ignorable level thus the challenging use.en_US
dc.description.sponsorshipEge University Scientific Research ProjectEge University [2015-TIP-025]en_US
dc.description.sponsorshipWe thank Specialist Erhan Canbay, from Ege University Medical Biochemistry Department, for his assistance with mass spectrometry analysis. Funding of this work by the Ege University Scientific Research Project (Funder Id: http://dx.doi.org/10.13039/501100003010, 2015-TIP-025) is gratefully acknowledged and Ege University Clinical Researches Ethical Committee ethical permission was taken (permit 14705/2015 15-4.1/17).en_US
dc.identifier.doi10.1515/tjb-2018-0413
dc.identifier.endpage744en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage738en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2018-0413
dc.identifier.urihttps://hdl.handle.net/11454/70525
dc.identifier.volume44en_US
dc.identifier.wosWOS:000501564800003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlycosaminoglycanen_US
dc.subjectChromatographyen_US
dc.subjectMolecular imprintingen_US
dc.subjectAcrylamido phenylboronic aciden_US
dc.subjectSolid phase extractionen_US
dc.titleDevelopment of molecularly imprinted Acrylamide-Acrylamido phenylboronic acid copolymer microbeads for selective glycosaminoglycan separation in children urineen_US
dc.typeArticleen_US

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