Exploitation of Agricultural Wastes and By-Products for Production of Aureobasidium pullulans Y-2311-1 Xylanase: Screening, Bioprocess Optimization and Scale Up

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dc.contributor.authorYegin, Sirma
dc.contributor.authorBuyukkileci, Ali Oguz
dc.contributor.authorSargin, Sayit
dc.contributor.authorGoksungur, Yekta
dc.date.accessioned2019-10-27T11:09:25Z
dc.date.available2019-10-27T11:09:25Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe potential of several agricultural wastes and by-products (wheat bran, oat bran, corn cob, brewer's spent grain, malt sprout, artichoke stem, sugar beet pulp, olive seed, cotton stalk and hazelnut skin) was examined as the substrate for xylanase production by Aureobasidium pullulans Y-2311-1. Based on the screening studies, wheat bran was selected as the best substrate for further optimization studies. The effects of initial medium pH, temperature and incubation time on xylanase production in shake flask system were optimized by response surface methodology (RSM). The optimum levels of the process variables defined by the model (initial medium pH, 4.24; temperature, 30.27 degrees C; and incubation time 126.67 h) resulted in production of 85.19 U/ml xylanase. Taking the RSM optimized parameters in shake-flask scale into consideration; xylanase production was scaled up to bioreactor system with a working volume of 1.5 l. The peak of enzyme production was achieved after 126 h incubation that has previously been determined by RSM studies at shake flask level. Furthermore, the optimum levels of agitation and aeration in bioreactor system was found as 200 rpm and 1.5 vvm. Maximum enzyme production was close to 85 kU/l which could be translated into a productivity of 0.68 kU/l/h. No previous work considered the statistical optimization of xylanase production by A. pullulans on wheat bran and scale up of the bioprocess to a bioreactor system.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey-TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 112O521]; Ege University Science and Technology Center-EBILTEMEge University [2013/BIL/025]en_US
dc.description.sponsorshipThis work was financially supported by The Scientific and Technological Research Council of Turkey-TUBITAK (Project No: TOVAG 112O521) and Ege University Science and Technology Center-EBILTEM (Project No: 2013/BIL/025). S. Yegin is a management committee member in Cost Action TD1203 entitled as Food Waste Valorization for Sustainable Chemicals, Materials and Fuels (EUBis).en_US
dc.identifier.doi10.1007/s12649-016-9646-6
dc.identifier.endpage1010en_US
dc.identifier.issn1877-2641
dc.identifier.issn1877-265X
dc.identifier.issn1877-2641en_US
dc.identifier.issn1877-265Xen_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage999en_US
dc.identifier.urihttps://doi.org/10.1007/s12649-016-9646-6
dc.identifier.urihttps://hdl.handle.net/11454/32286
dc.identifier.volume8en_US
dc.identifier.wosWOS:000398594100040en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWaste and Biomass Valorizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBy-product valorizationen_US
dc.subjectXylanaseen_US
dc.subjectAureobasidium pullulansen_US
dc.subjectBioprocess optimizationen_US
dc.subjectBioreactoren_US
dc.titleExploitation of Agricultural Wastes and By-Products for Production of Aureobasidium pullulans Y-2311-1 Xylanase: Screening, Bioprocess Optimization and Scale Upen_US
dc.typeArticleen_US

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