A novel immobilization matrix for the biosensing of phenol: self assembled monolayers of calixarenes

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dc.contributor.authorTasci, Filiz
dc.contributor.authorSayin, Serkan
dc.contributor.authorSeleci, Didem Ag
dc.contributor.authorDemir, Bilal
dc.contributor.authorAzak, Hacer
dc.contributor.authorYildiz, Huseyin Bekir
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T11:08:48Z
dc.date.available2019-10-27T11:08:48Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: The development of calixarene based phenol biosensor. Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1- oxypropane)-26,28-dihydroxycalix[4] arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy interaction with Au surface and one-step biomolecule immobilization. Self-assembled monolayers (SAMs) of p-amino-functionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) enzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2/Lac) was used for the electrochemical analysis of phenolic compounds at -50 mV. Results: The linearity was observed in the range of 0.1-100 mu M and 1.0-100 mu M for catechol and phenol, respectively. The potential use of the biosensor was investigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step. Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic contaminations in samples.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-2209]; European Union through the COST Action "Supramolecular photocatalytic water splitting (PERSPECT-H2O)" [CM1202]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113T022]; Turkish Academy of Sciences-Outstanding Young Scientists Award Program (TUBA-GEBIP)Turkish Academy of Sciencesen_US
dc.description.sponsorshipDr. D.O. Demirkol thanks to The Turkish Academy of Sciences-Outstanding Young Scientists Award Program (TUBA-GEBIP 2015). The authors also would like to thank TUBITAK for its Supporting Research Projects Program for the University Students at undergraduate level (Program Number TUBITAK-2209). This study was partially supported by the European Union through the COST Action CM1202 "Supramolecular photocatalytic water splitting (PERSPECT-H2O)" and the Scientific and Technological Research Council of Turkey (TUBITAK Grant Number 113T022).en_US
dc.identifier.doi10.1515/tjb-2016-0178
dc.identifier.endpage236en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue2en_US
dc.identifier.startpage229en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0178
dc.identifier.urihttps://hdl.handle.net/11454/32217
dc.identifier.volume42en_US
dc.identifier.wosWOS:000405114700014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFunctionalized calixareneen_US
dc.subjectLaccaseen_US
dc.subjectPhenol detectionen_US
dc.subjectAmperometric biosensoren_US
dc.subjectImmobilization of enzymesen_US
dc.titleA novel immobilization matrix for the biosensing of phenol: self assembled monolayers of calixarenesen_US
dc.typeArticleen_US

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