Effect of resveratrol on apoptosis and MDM2, RUNX3, RB gene expressions in human acute myeloid leukemia cells by transfection of MATRA-mediated miR-150 [MATRA aracılı miR-150’nin transfeksiyonu ile insan akut myeloid lösemi hücrelerinde resveratrol’ün MDM2,RUNX3,RB gen ekspresyonları ve apoptoz üzerine etkisi]

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dc.contributor.authorOkcanoglu T.B.
dc.contributor.authorSusluer S.Y.
dc.contributor.authorKayabasi C.
dc.contributor.authorYelken B.O.
dc.contributor.authorSaydam, G..
dc.contributor.authorAvci C.B.
dc.contributor.authorGunduz C.
dc.date.accessioned2019-10-27T08:02:47Z
dc.date.available2019-10-27T08:02:47Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractAcute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies. Magnet assisted transfection (MATRA) is one of the most effective non-viral transfection methods. We aimed to evaluate the apoptotic effect of resveratrol (RES), MDM2, RUNX3, RB gene expression exchanges and MicroRNA-150 (miR-150) transfected with MATRA to HL-60 cells. MATRA was used as non-viral vector carrier for miR-150 transfection. IC50 dose of RES was determineted by Fahri et al. Resveratrol and miR-150 transfected cells were performed apoptosis and MDM2, RUNX3, RB gene expression assays in Human promyelocytic leukemia (HL-60) cells. IC50 dose of RES was used as 5 µM. In HL-60 cells, it was found that miR-150, miR150-Resveratrol combination, resveratrol alone induces apoptosis by 6.48, 6.93 and 4.54 fold, respectively, compared to the control cells. Compared to control cells, MDM2, RUNX3, RB, gene expressions decreased (miR-150) 1.7, 1.3 and 1.4 fold, (miR-150-resveratrol) MDM2 expression increased 2.73 fold, RUNX3 and RB expression decreased 6.1, 1.07 fold, respectively. Combination with resveratrol, MDM2, RB decreased 2.9 and 1.4 fold, respectively. Non-viral miR-150 transfection may be effective in leukemia cells, induction of apoptotic effects and gene expression changes, following treatment with resveratrol and miR-150-resveratrol combinations. © 2018, UHOD - Uluslararasi Hematoloji Onkoloji Dergisi. All rights reserved.en_US
dc.identifier.doi10.4999/uhod.182713
dc.identifier.endpage153en_US
dc.identifier.issn1306133X
dc.identifier.issn1306-133Xen_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org/10.4999/uhod.182713
dc.identifier.urihttps://hdl.handle.net/11454/25246
dc.identifier.volume28en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUHOD - Uluslararasi Hematoloji Onkoloji Dergisien_US
dc.relation.ispartofUHOD - Uluslararasi Hematoloji-Onkoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcute myeloid leukemiaen_US
dc.subjectGene expressionsen_US
dc.subjectMATRAen_US
dc.subjectmiR-150en_US
dc.subjectResveratrolen_US
dc.titleEffect of resveratrol on apoptosis and MDM2, RUNX3, RB gene expressions in human acute myeloid leukemia cells by transfection of MATRA-mediated miR-150 [MATRA aracılı miR-150’nin transfeksiyonu ile insan akut myeloid lösemi hücrelerinde resveratrol’ün MDM2,RUNX3,RB gen ekspresyonları ve apoptoz üzerine etkisi]en_US
dc.typeArticleen_US

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