Rapid profiling and identification of triterpene saponins in three different Cephalaria species by UPLC-ESI-MS/MS

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dc.contributor.authorCelenk V.U.
dc.contributor.authorSarikahya N.B.
dc.contributor.authorKirmizigul S.
dc.date.accessioned2019-10-26T21:14:40Z
dc.date.available2019-10-26T21:14:40Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractTriterpene saponins are bioactive glycosides which are responsible for the defense mechanism of the plants and have extensive structural diversity in many plant species. In this study, profile of triterpene saponins was determined in the crude n-BuOH extracts of Cephalaria hirsuta, Cephalaria elazigensis var. elazigensis, and Cephalaria procera taxa (Caprifoliaceae) by ultra pressure liquid chromatography coupled with electrospray ionization tandem mass spectrometry. The UPLC-ESI-MS/MS method has been validated to provide linearity, recovery, precision (repeatability), limit of detection and limit of quantification parameters for quantitative analysis. Identification and quantification of the compounds were based on a group of fragment ions and ESI-MS spectra; precursor ions were observed as [M+Na]+ and [M+H]+ in positive mode for molecular mass information. Thirty nine saponins were identified and quantified by reversed-phase liquid chromatography (RP-LC) and they were separated on C18 reverse phase column (50 mm x 2.1 mm, 1.7 µm) by methanol-water gradient containing 0.1 mM ammonium formate solution as a mobile phase. Among the detected compounds, 28 compounds in C. hirsuta, 4 compounds in C. elazigensis var. elazigensis and 22 compounds in C. procera were identified and quantified based on their retention times and mass spectra in comparison with the data of references which were isolated in our previous studies. In this study, rapid application of an UPLC-ESI-MS/MS approach for resolving the chemical content of complex crude n-BuOH extracts of three Cephalaria species were presented, for the first time. © 2018 ACG Publications. All rights reserved.en_US
dc.description.sponsorshipUniversity of Delaware Research Foundation: 2015/FEN/061en_US
dc.description.sponsorshipThe authors gratefully thank Ege University, Center for Drug Research and Development and Pharmacokinetic Applications (ARGEFAR) for providing Waters TQD UPLC-ESI-MS/MS system and Ege University Research Foundation (2015/FEN/061) for financial support. --en_US
dc.identifier.doi10.25135/rnp.45.17.10.179
dc.identifier.endpage469en_US
dc.identifier.issn1307-6167
dc.identifier.issn1307-6167en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage460en_US
dc.identifier.urihttps://doi.org/10.25135/rnp.45.17.10.179
dc.identifier.urihttps://hdl.handle.net/11454/15880
dc.identifier.volume12en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherACG Publicationsen_US
dc.relation.ispartofRecords of Natural Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCephalariaen_US
dc.subjectSaponinen_US
dc.subjectUPLC-ESI-MS/MSen_US
dc.titleRapid profiling and identification of triterpene saponins in three different Cephalaria species by UPLC-ESI-MS/MSen_US
dc.typeArticleen_US

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