Determination of Total Protein and Free Amino Acid Content of ArtemisiaAbrotanum L. in the Blooming and Pre-Blooming Period

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dc.contributor.authorCansever, Islam
dc.contributor.authorSoeguet, Oezlem
dc.date.accessioned2023-01-12T20:10:46Z
dc.date.available2023-01-12T20:10:46Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractObjective: The aim of this study is to determine the total protein and free amino acid content of Artemisia abrotanum L. in two different periods, the pre-blooming and blooming periods.Methods: The Dumas and Kjeldahl methods were applied comparatively to determine the total protein amount. In addition, after the sample was extracted with water:methanol (80:20) containing 0.1% formic acid, free amino acids were determined in liquid chromatography-mass spectrometer.: By using the Dumas method, the % nitrogen content was found to be 2.10 and 2.20, in the pre-blooming and blooming period samples. The results obtained by applying the Kjeldahl method are 2.16 and 2.25, respectively. The total protein content was calculated from the nitrogen content of the plant by using a nitrogen conversion factor of 6.25. The total free amino acid content in the pre-blooming and blooming period was found to 453.41 and 606.18 mg/100 g dried plant respectively.Conclusion: Since the total protein and free amino acid content of Artemisia abrotanum L. is higher during the blooming period, it should be preferred to be harvested during this period.Keywords: Artemisia abrotanum, Kjeldahl method, Dumas method, protein, free amino acid, LC-MS/MSen_US
dc.description.sponsorshipEge University Office of Scientific Research Projects; [TDK-2019-250585]en_US
dc.description.sponsorshipAcknowledgements: This study was supported by Ege University Office of Scientific Research Projects (TDK-2019-250585) .en_US
dc.identifier.doi10.14235/bas.galenos.2021.6688
dc.identifier.endpage627en_US
dc.identifier.issn2148-2373
dc.identifier.issn2148-2373en_US
dc.identifier.issue5en_US
dc.identifier.startpage623en_US
dc.identifier.urihttps://doi.org/10.14235/bas.galenos.2021.6688
dc.identifier.urihttps://hdl.handle.net/11454/77918
dc.identifier.volume10en_US
dc.identifier.wosWOS:000877834200013en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofBezmialem Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectArtemisia abrotanumen_US
dc.subjectKjeldahl methoden_US
dc.subjectDumas methoden_US
dc.subjectproteinen_US
dc.subjectfree amino aciden_US
dc.subjectLC-MSen_US
dc.subjectMSen_US
dc.subjectSamplesen_US
dc.titleDetermination of Total Protein and Free Amino Acid Content of ArtemisiaAbrotanum L. in the Blooming and Pre-Blooming Perioden_US
dc.typeArticleen_US

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