Amplified electrochemical DNA-sensing of nanostructured metal oxide films deposited on disposable graphite electrodes functionalized by chemical vapor deposition

Küçük Resim Yok

Tarih

2009

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Elsevier Science Sa

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Metal oxide nanostructures offer interesting possibilities to design functional surfaces for biosensing applications, for instance, through higher surface area leading to enhanced immobilization of biomolecules, which increases the detection limit. Herein, an amplified electrochemical sensing method has been presented for the detection of DNA based on the readout resulting from chemical oxidation of guanine on nanoscaled metal oxides (TiO(2), SnO(2) and Fe(3)O(4)) obtained by chemical vapor deposition (CVD) onto pencil graphite electrode (PGE) as electrochemical transducer. The proposed strategy is suitable to produce cost-effective disposable sensor elements enabling quantitative detection of nanomolar concentrations of DNA. When preparing these metal oxide surfaces by CVD onto PGEs, the various experimental conditions; such as, the effect of different concentrations of 20 mer-bases DNA oligonucleotide (ODN20), and the surface pretreatment steps were Studied to obtain better surface properties for DNA immobilization. The detection limit estimated for signal-to-noise ratios >3 corresponds to 21.3, 519 and 45.8 nmole/ml ODN20 concentrations for PGEs modified with TiO(2), SnO(2) and Fe(3)O(4) films, respectively. The electrochemical detection of DNA onto metal oxide@PGEs is discussed together with the application potential. (c) 2008 Elsevier B.V. All rights reserved.

Açıklama

Anahtar Kelimeler

DNA, Nanostructured metal oxides, Chemical vapor deposition (CVD), Metal oxide-modified electrodes, Pencil graphite electrodes (PGEs), Guanine oxidation signal

Kaynak

Sensors and Actuators B-Chemical

WoS Q Değeri

Q1

Scopus Q Değeri

N/A

Cilt

136

Sayı

2

Künye