miR-26a and miR-125b as Indicators for the Severity of SARS-CoV-2Infectious: A Case-Control Study

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dc.authorid0000-0001-7846-2908
dc.contributor.authorJaberi, Milad
dc.contributor.authorAsadi, Milad
dc.contributor.authorZarredar, Habib
dc.contributor.authorShanehbandi, Dariush
dc.contributor.authorZafari, Venus
dc.contributor.authorPouresmaeil, Ahmad
dc.contributor.authorSharifi, Akbar
dc.date.accessioned2025-03-26T08:19:27Z
dc.date.available2025-03-26T08:19:27Z
dc.date.issued2024
dc.departmentEge Üniversitesi, Sağlık Bilimleri Enstitüsü, Radyasyon Onkolojisi Ana Bilim Dalı
dc.description.abstractBackground: The recent SARS-CoV-2 coronavirus pandemic has spread worldwide, with the first cases recorded in December2019 in China. Symptoms include fever, cough, dyspnea, and gastrointestinal issues. Patients with SARS-CoV-2 are classified as mild, severe, or critical, highlighting the necessity for earlier treatment methods and reliable biomarkers. MicroRNAs have consistently been considered essential biological biomarkers. Objectives: In this case-control study, we investigated the levels of miR-26a and miR-125b in PBMCs of SARS-CoV-2 patients in northwestern Iran. Methods: Blood samples were collected from 100 COVID-19-infected patients, divided into two groups: 50 patients with no serious lung damage and 50 patients with COVID-19 infection and a lung abscess. RNA extraction techniques were used to examine PBMC specimens from COVID-19 patients. mRNA was extracted from WBCs, and the expression levels of miR-26a andmiR-125b were evaluated using qRT-PCR in the two study groups. Results: The expression levels of miR-26a and miR-125b in PBMCs of patients with a poor prognosis were significantly lower compared to those with a good prognosis, as shown by qRT-PCR. The receiver operating characteristic (ROC) analysis indicated significant diagnostic potential for both miRNAs (miR-26a [area under the ROC curve (AUC)] = 0.8458, P < 0.0001; miR-125b AUC= 0.7944, P < 0.0001). Conclusions: The relative expression of miR-26a and miR-125b in SARS-CoV-2 infection was significantly different between the two study groups. These findings suggest their potential as disease severity indicators and prognostic markers.
dc.identifier.citationJaberi, M., Asadi, M., Zarredar, H., Shanehbandi, D., Zafari, V., Pouresmaeil, A., & Sharifi, A. (2024). miR-26a and miR-125b as indicators for the severity of SARS-CoV-2 infectious: A case-control study. Jundishapur Journal of Microbiology, 17(11)
dc.identifier.doi10.5812/jjm-154546
dc.identifier.endpage7
dc.identifier.issn2008-3645
dc.identifier.issue11
dc.identifier.scopus2-s2.0-85215430298
dc.identifier.scopusqualityQ4
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.5812/jjm-154546
dc.identifier.urihttps://hdl.handle.net/11454/116935
dc.identifier.volume17
dc.identifier.wosWOS:001381893500002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAsadi, Milad
dc.institutionauthorid0000-0001-7846-2908
dc.language.isoen
dc.publisherBrieflands
dc.relation.ispartofJundishapur Journal of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmicRNA-125b
dc.subjectmicRNA-26a
dc.subjectPBMCs
dc.subjectSARS-CoV-2
dc.titlemiR-26a and miR-125b as Indicators for the Severity of SARS-CoV-2Infectious: A Case-Control Study
dc.typeArticle

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