Labeling bombesin-like peptide with 99mTc via hydrazinonicotinamide: Description of optimized radiolabeling conditions

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dc.contributor.authorLambrecht F.Y.
dc.contributor.authorDurkan K.
dc.contributor.authorBayrak E.
dc.date.accessioned2019-10-27T08:34:51Z
dc.date.available2019-10-27T08:34:51Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractBombesin (BNN)-like peptides have very high binding affinity for the gastrin-releasing peptide (GRP) receptor. The goal of the current study was to optimize the labeling conditions of a new 99mTc-radiolabeled BNN-like peptide based on the bifunctional chelating ligand HYNIC using different co-ligands (EDDA and tricine). The radiolabeling conditions (pH, amount of co-ligand, amount of stannous chloride, temperature and reaction time) for newly-formed 99mTc-tricine-HYNIC-Q-Litorin and 99mTc-EDDA- HYNIC-Q-Litorin were optimized and evaluated by RHPLC and RTLC. Radiochemical yields for 99mTc-tricine-HYNIC-Q-Litorin and 99mTc-EDDA- HYNIC-Q-Litorin were 98.0 ± 1.7 and 97.5 ± 2.5%, respectively. When EDDA was used as co-ligand, the labeling of 99mTc-EDDA-HYNIC-Q- Litorin was optimal in the following reaction mixture: HYNIC-peptide: EDDA: 10 µg/5 mg, pH 3, SnCl2 concentration: 12 µg/0.1 mL, reaction temperature: 100 °C, reaction time: 15 min. Besides, the optimum conditions were HYNIC-peptide:tricine: 10 µg/50 mg, pH 5, SnCl2 concentration: 12 µg/0.1 mL, reaction temperature: 100 °C, reaction time: 15 min for preparing 99mTc-tricine-HYNIC-Q-Litorin. The manufactured 99mTc-HYNIC-Q-Litorin conjugates may offer new possibilities for imaging cancer cells expressing bombesin receptors. © 2010 Akadémiai Kiadó.en_US
dc.description.sponsorshipTUBITAK Number, 108S200en_US
dc.description.sponsorshipAcknowledgements The authors gratefully acknowledge the financial support received from the Scientific and Technological Research Council of Turkey, Scientific Project (TUBITAK Number 108S200). --en_US
dc.identifier.doi10.1007/s10967-010-0530-8
dc.identifier.endpage545en_US
dc.identifier.issn0236-5731
dc.identifier.issn0236-5731en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage539en_US
dc.identifier.urihttps://doi.org/10.1007/s10967-010-0530-8
dc.identifier.urihttps://hdl.handle.net/11454/27122
dc.identifier.volume284en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Radioanalytical and Nuclear Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject99mTcen_US
dc.subjectCo-liganden_US
dc.subjectGastrin-releasing peptideen_US
dc.subjectHYNIC-BNN-like peptideen_US
dc.subjectLitorinen_US
dc.titleLabeling bombesin-like peptide with 99mTc via hydrazinonicotinamide: Description of optimized radiolabeling conditionsen_US
dc.typeArticleen_US

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