Gas chromatographic analysis of alcohols in blood with a chemometric approach

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dc.contributor.authorErtas, Hasan
dc.contributor.authorOzturk, Pembe
dc.contributor.authorErdem, Asli
dc.contributor.authorAkpolat, Oguz
dc.contributor.authorAkgur, Serap A.
dc.contributor.authorErtas, F. Nil
dc.date.accessioned2019-10-27T21:42:39Z
dc.date.available2019-10-27T21:42:39Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractThe objective of the present study is to examine the main and interactive effects of different factors related with headspace-solid phase extraction (HS-SPME) and gas chromatography (GC) conditions for the simultaneous determination of alcohols (ethanol, methanol, isopropanol, n-propanol) and acetone in blood samples. In order to evaluate the simultaneous effects of the significant variables, a chemometric approach was utilized to reveal the important parameters. The factors affecting the headspace extraction of alcohols on the SPME fiber was first deduced in a screening study by applying a Plackett-Burman design. According to the screening results, the factors that possess a positive effect on peak areas are sample concentration, inlet temperature, flow rate for the carrier gas and volume of the solution. Based on the results of the screening study, to continue optimization, six insignificant values were fixed at the appropriate amounts (oven temperature: 50 degrees C; detector temperature: 250 degrees C; H-2 gas flow rate: 35 mL min(-1); split flow rate: 30 mL min(-1); equilibrium temperature: 55 degrees C, extraction time: 4 min). Subsequently, a central composite design (CCD) was constructed for optimization and response surface methodology (RSM) was used to interpret the effect of each pair of independent variables on the response. The optimized method for alcohol and acetone analysis via HS-SPME-GC was validated through spike recovery tests.en_US
dc.description.sponsorshipEge University Research FoundationEge University [2002/037]; TubitakTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-HD/6 (105T017)]en_US
dc.description.sponsorshipAuthors would like to thank Ege University Research Foundation (Project no 2002/037) and Tubitak (TBAG-HD/6 (105T017)) for financial support.en_US
dc.identifier.doi10.1039/c3ay40628h
dc.identifier.endpage5178en_US
dc.identifier.issn1759-9660
dc.identifier.issn1759-9660en_US
dc.identifier.issue19en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage5172en_US
dc.identifier.urihttps://doi.org/10.1039/c3ay40628h
dc.identifier.urihttps://hdl.handle.net/11454/46936
dc.identifier.volume5en_US
dc.identifier.wosWOS:000324489100031en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalytical Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleGas chromatographic analysis of alcohols in blood with a chemometric approachen_US
dc.typeArticleen_US

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