Preparation, characterization and evaluation of solid lipid nanoparticles and niosomes for ING4 gene delivery to MCF-7 cells
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marmara Univ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, we aimed to develop lipid-based delivery systems for ING4 gene. For this purpose, we chose solid lipid nanoparticle (SLN) and niosome formulations for their advantages in several ways. SLNs were prepared via hot microemulsion method with slight modifications. Niosomes were prepared by using thin film hydration method. Both of those formulations contain cationic lipids for the purpose of gaining positive charge. Thus, complexation with ING4 gene carrier plasmid DNA is conducted via electrostatic interactions. DLS measurements showed us both of those formulations have appropriate zeta potential for complexation which is above 30 mV. Average particle size of SLNs is 9.76 +/- 0.13 nm. Vesicle size of niosomes is 483.2 +/- 20.19 nm. Both of those formulations can be considered nano-sized delivery systems. Reliability of those values were supported with TEM imaging. DNA protection ability of those formulations were observed in serum stability study. After characterization studies, cytotoxic effect of those delivery systems was evaluated on MCF-7 cell lines.
Açıklama
Anahtar Kelimeler
ING4, solid lipid nanoparticles, niosomes, gene delivery
Kaynak
Journal of Research in Pharmacy
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
23
Sayı
5