Intra-laboratory validation of commercially available ELFA-based assay and PCR-based assay for detection of Listeria monocytogenes in food samples according to ISO 16140-2
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Vup Food Research Inst, Bratislava
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, commercially available VIDAS system based on the enzyme linked fluorescent assay (ELFA; BioMerieux, Lyon, France) and the BAX system based on polymerase chain reaction (PCR; Hygiena, Camarillo, California, USA) were compared to the reference culture-based method (EN ISO 11290-1) to detect Listeria monocytogenes in food samples. The process was carried out in accordance with criteria for methods comparison established in EN ISO 16140-2 for validation of alternative microbiological methods. As a result of validation studies with food samples, sensitivity rates for samples in all food categories were determined as 97.2 % for the ELFA method and 97.9 % for the PCR method. Level of detection of the ELFA method per 25 g was determined to be 1.1-4.3 CFU and of the PCR method was determined to be 2.7-8.0 CFU. The method comparison study is a part of the validation process that takes place in analysis laboratories. Although the VIDAS System and BAX System have been used in food analysis laboratories for many years, this study is the first intralaboratory method validation study conducted in accordance with the latest version of ISO 16140-2 against a reference method.
Açıklama
Anahtar Kelimeler
Listeria Monocytogenes, Rapid Microbiological Method, Intra-Laboratory Validation, Enzyme-Linked Flouorescent Immu-Noassay, Polymerase Chain Reaction
Kaynak
Journal of Food and Nutrition Research
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
62
Sayı
4
