Dye Attached Nanoparticles for Lysozyme Adsorption

dc.contributor.authorUygun, Murat
dc.contributor.authorUygun, Deniz Aktas
dc.contributor.authorAltunbas, Canan
dc.contributor.authorAkgol, Sinan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-27T22:14:58Z
dc.date.available2019-10-27T22:14:58Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this work, Reactive Blue 15 dye functionalized poly(HEMA) nanoparticles were synthesized for reversible adsorption of lysozyme from its aqueous solution. For this, nano-sized poly(HEMA) nanoparticles were synthesized by the surfactant free emulsion polymerization. Reactive Blue 15 dye then covalently attached to the polymeric structure. These novel dye attached poly(HEMA) nanoparticles were used for the adsorption of lysozyme. Characterization of dye attached nanoparticles was carried out by using FTIR, AFM, and elemental analysis. Incorporation of the dye onto the polymeric structure was demonstrated by FTIR and elemental analysis, while the size and the shape of the nanoparticles were shown by AFM. The incorporated amount of the dye was found to be 70.3 mu mol/g nanoparticle with sulphur stoichiometry and it was found that the prepared nanoparticles were in a spherical form and were about 100nm diameter. Lysozyme adsorption studies were carried out with different conditions (pH, lysozyme concentration, temperature, and ionic strength) and maximum lysozyme adsorption was found to be 630.7mg/g nanoparticle at pH 7.0 in 25 degrees C medium temperature. Adsorbed lysozyme was desorbed by 1.0M of NaCl with 96% recovery and synthesized dye-attached nanoparticles were used 10 times without any decrease in their adsorption capacity.en_US
dc.identifier.doi10.1080/01496395.2013.878723
dc.identifier.endpage1278en_US
dc.identifier.issn0149-6395
dc.identifier.issn1520-5754
dc.identifier.issn0149-6395en_US
dc.identifier.issn1520-5754en_US
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1270en_US
dc.identifier.urihttps://doi.org/10.1080/01496395.2013.878723
dc.identifier.urihttps://hdl.handle.net/11454/50097
dc.identifier.volume49en_US
dc.identifier.wosWOS:000335945400017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofSeparation Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectReactive Blue 15en_US
dc.subjectnanoparticleen_US
dc.subjectlysozymeen_US
dc.subjectdye-liganden_US
dc.titleDye Attached Nanoparticles for Lysozyme Adsorptionen_US
dc.typeArticleen_US

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