Converted carotenoid production in Dunaliella salina by using cyclization inhibitors 2-methylimidazole and 3-amino-1,2,4-triazole

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dc.contributor.authorYildirim, Arzu
dc.contributor.authorAkgun, Ismail Hakki
dc.contributor.authorDalay, Meltem Conk
dc.date.accessioned2019-10-27T11:22:37Z
dc.date.available2019-10-27T11:22:37Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractCarotenoids are vital for most photosynthetic organisms because of their crucial role in prevention of the damage caused by excess light or stress conditions like heat or nutrient deprivation. Some of them are also valuable for biotechnology with respect to their colorful feature and antioxidant properties. In this study, a natural beta-carotene producer, green microalga Dunaliella salina, was evaluated for its potential to produce different valuable carotenoids through the inhibition of cyclization reactions in the carotenoid pathway by the chemical inhibitors 2-methylimidazole (2MI) and 3-amino-1,2,4-triazole (Amitrol). 2MI was shown to be effective on lutein accumulation in D. salina cultures grown at high temperature, without affecting cell viability. Addition of 2MI to the culture at 1 mM concentration resulted a 1.7-fold increase in lutein content in the cell with the highest amount of 3.45 pg/cell, and an associated decrease in beta-carotene content suggesting that this inhibitor is more effective on lycopene beta-cyclase activity, thus shifting the pathway from beta-carotene arm to the alpha-carotene direction. The results of this study may give the opportunity to use D. salina for the production of valuable carotenoids other than beta-carotene.en_US
dc.description.sponsorshipTurkish General Directorate of Agricultural Research and Policy [TAGEM/10/ARGE/06]en_US
dc.description.sponsorshipThis work was supported by the projects from the Turkish General Directorate of Agricultural Research and Policy (TAGEM/10/ARGE/06).en_US
dc.identifier.doi10.3906/biy-1606-10
dc.identifier.endpage219en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issn1300-0152en_US
dc.identifier.issn1303-6092en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage213en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1606-10
dc.identifier.urihttps://hdl.handle.net/11454/33057
dc.identifier.volume41en_US
dc.identifier.wosWOS:000394539800022en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDunaliella salinaen_US
dc.subjectcarotenoidsen_US
dc.subjectchemical inhibitorsen_US
dc.subjectluteinen_US
dc.titleConverted carotenoid production in Dunaliella salina by using cyclization inhibitors 2-methylimidazole and 3-amino-1,2,4-triazoleen_US
dc.typeArticleen_US

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