A New Experimental Allergic Rhinitis Model in Mice

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dc.contributor.authorKose, Sukran
dc.contributor.authorKis, Tuba Tatli
dc.contributor.authorDiniz, Gulden
dc.contributor.authorAkbulut, Ilkay
dc.contributor.authorSerin, Basak Gol
dc.contributor.authorYilmaz, Canberk
dc.contributor.authorOzyazici, Mine
dc.date.accessioned2023-01-12T20:18:49Z
dc.date.available2023-01-12T20:18:49Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractObjective: Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa mediated by IgE after exposure to an allergen. The most well known related comorbidity of AR is asthma. This study was planned due to the need for an animal model for studies on AR-asthma coexistence. In this study, the frequency of AR accompanying in the asthma model created in mice,and the usability of the related model in AR studies will be investigated. Methods: In our study, 6-8 week-old, 18-20 g BALB/c mice were used. Chicken egg ovalbumin (OVA Grade V, Sigma) was administered through intraperitoneal (IP) route at doses of 10 mu g on days 0 and 14. Mice were exposed to aerosolized 2.5% ovalbumin solution in sterile saline for 30 minutes 3 days a week for 8 weeks, starting 7 days after the last IP administration (21st day). After exposure to OVA, mice were observed for typical signs of AR including sneezing, runny nose, and nasal itching. The final diagnosis of AR was made by histopathological examination of the rhinotracheal tissues of mice. Results: In our study, all mice exposed to ovalbumin received histopathologic diagnosis of AR. Increased number of capillaries lymphocytes, polymorphonuclear leukocytes and eosinophilsper square millimetre of rhinotracheal tissues were calculated in the murine model of AR compared to the the control group. Conclusion: This study introduced a new AR model, not cited in the literature, and induced with the longest-term ovalbumin exposure in the literature. It was concluded that this model, known as the asthma model, can also be used to induce an AR model and can be used in studies investigating coexistence of allergic rhinitis and asthma.en_US
dc.identifier.doi10.5222/buchd.2021.86658
dc.identifier.endpage239en_US
dc.identifier.issn2146-2372
dc.identifier.issn1309-9566
dc.identifier.issn2146-2372en_US
dc.identifier.issn1309-9566en_US
dc.identifier.issue3en_US
dc.identifier.startpage233en_US
dc.identifier.urihttps://doi.org/10.5222/buchd.2021.86658
dc.identifier.urihttps://hdl.handle.net/11454/78934
dc.identifier.volume11en_US
dc.identifier.wosWOS:000729737700003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofIzmir Dr Behcet Uz Cocuk Hastanesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllergic rhinitisen_US
dc.subjectasthmaen_US
dc.subjectmouseen_US
dc.subjectMurine Modelen_US
dc.subjectAsthmaen_US
dc.subjectRisken_US
dc.titleA New Experimental Allergic Rhinitis Model in Miceen_US
dc.typeArticleen_US

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