Ubiquitin-Proteasome Axis, Especially Ubiquitin-Specific Protease-17 (USP17) Gene Family, is a Potential Target for Epithelial-Mesenchymal Transition in High-Grade Serous Ovarian Cancer

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dc.contributor.authorYildirim, Nuri
dc.contributor.authorKocal, Gizem Calibasi
dc.contributor.authorIsik, Zerrin
dc.contributor.authorSaatli, Bahadir
dc.contributor.authorSaygili, Ugur
dc.contributor.authorUysal, Tugba
dc.contributor.authorUlukus, Cagnur
dc.contributor.authorKoyuncuoglu, Meral
dc.contributor.authorEllidokuz, Hulya
dc.contributor.authorBasbinar, Yasemin
dc.date.accessioned2019-10-27T09:43:43Z
dc.date.available2019-10-27T09:43:43Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives: To investigate gene expression differences and related functions between primary tumor, malignant cells in ascites, and metastatic peritoneal implant in high-grade serous ovarian cancer. Methods: Biopsies from primary tumor, peritoneal implant, and ascites were collected from 10 patients operated primarily for high-grade, advanced-staged serous ovarian cancer. Total RNA isolation was performed from collected tissue biopsy and fluid samples, and RNA expression profile was measured. Messenger RNA expression profiles of 3 different groups were compared. Functional analyses of candidate genes were carried out by gene ontology and pathway analysis. Results: There were significant differences in the expression of 5 genes between primary tumor and peritoneal implant, 979 genes between primary tumor and malignant cells in ascites, and 649 genes between peritoneal implant and malignant cells in ascites. Three commonly enriched gene ontology functions between "primary tumor and malignant cells in the ascites" and "peritoneal implant and malignant cells in the ascites" were protein deubiquitination, ubiquitin-dependent protein catabolism, and apoptotic processes. All genes related to these functions belonged to USP17 gene family. Conclusion: Gene expression difference between primary tumor and the peritoneal implant is not as much as the difference between primary tumor and free cells in the ascites. These results show that malignant cells in the ascites return into its genetic origin after they invade on the peritoneum. Significantly increased expression of DUB-enzyme genes, SNAR gene family, and ribosomal pathway genes in epithelial-mesenchymal transition suggests that this regulation is ubiquitin-proteasome dependent. Especially, this is the first study that offers USP17 as a potential target for epithelial-mesenchymal transition.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [214S334]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The study was supported by "The Scientific and Technological Research Council of Turkey (TUBITAK)" with the grant number 214S334.en_US
dc.identifier.doi10.1177/1933719118799189
dc.identifier.endpage805en_US
dc.identifier.issn1933-7191
dc.identifier.issn1933-7205
dc.identifier.issn1933-7191en_US
dc.identifier.issn1933-7205en_US
dc.identifier.issue6en_US
dc.identifier.startpage794en_US
dc.identifier.urihttps://doi.org/10.1177/1933719118799189
dc.identifier.urihttps://hdl.handle.net/11454/28903
dc.identifier.volume26en_US
dc.identifier.wosWOS:000475454600009en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSage Publications Incen_US
dc.relation.ispartofReproductive Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectovarian canceren_US
dc.subjectserousen_US
dc.subjectmicroarrayen_US
dc.subjectepithelial-mesenchymal transitionen_US
dc.subjectdeubiquitinationen_US
dc.subjectubiquitin-proteasome pathwayen_US
dc.titleUbiquitin-Proteasome Axis, Especially Ubiquitin-Specific Protease-17 (USP17) Gene Family, is a Potential Target for Epithelial-Mesenchymal Transition in High-Grade Serous Ovarian Canceren_US
dc.typeArticleen_US

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