Synthesis and application of a novel poly-L-phenylalanine electroactive macromonomer as matrix for the biosensing of 'Abused Drug' model

Basit Öğe Kaydı
dc.contributor.authorYilmaz, Tulay
dc.contributor.authorGuler, Emine
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorAkbulut, Huseyin
dc.contributor.authorAldemir, Ebru
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorColak, Demet Goen
dc.contributor.authorCianga, Ioan
dc.contributor.authorYamada, Shuhei
dc.contributor.authorTimur, Suna
dc.contributor.authorEndo, Takeshi
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-10-27T23:12:51Z
dc.date.available2019-10-27T23:12:51Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractPolypeptide-functionalized macromonomers are very fascinating candidates for the modification of various surfaces of different sizes as well as geometry, and have attracted considerable attention for biomolecule stabilization, biomedical device fabrication and bioanalytical applications. In the present work, synthesis and characterization of a novel poly-L-phenylalanine-bearing electroactive macromonomer (EDOT-BTDA-PPhe) were carried out. In the following steps, a glassy carbon electrode was covered with this material, and then cocaine aptamer was immobilized to obtain a biofunctional surface for the biosensing of ` Abused Drug' model. Aptamers attached to polypeptide side chains on the macromonomer with good orientation are induced for conformational change into three-way junction form as a result of selective binding of cocaine or its metabolite. This aptamer folding-based conformational response provides detectable signals due to formation of a compact interface which restricts electron transfer of redox probe. The stepwise modification of the surface was confirmed by electrochemical techniques. At the final step, the aptasensor was applied for the electrochemical detection of cocaine and its major metabolite, benzoylecgonine (BE), which exhibited a linear correlation between 1.0 and 10 nM and between 0.5 and 10 mu M respectively. The proposed methods were successfully employed for the analysis of synthetic biological fluids.en_US
dc.description.sponsorshipIndustrial Thesis Support Program (SAN-TEZ) of Republic of TurkeyMinistry of Science, Industry & Technology - Turkey; Ministry of Science, Industry and Technology [0620.STZ.2014]; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016K121190]; Ege University Research Fund through BAP project [16-FEN-004]; Istanbul Technical University Research Fund; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by Industrial Thesis Support Program (SAN-TEZ) of Republic of Turkey, Ministry of Science, Industry and Technology (Project Grant No. 0620.STZ.2014) and Republic of Turkey, Ministry of Development (Project Grant No. 2016K121190). This work was also partially supported by Ege University Research Fund through BAP project (Project No. 16-FEN-004) and Istanbul Technical University Research Fund. Likrom Company is acknowledged for its support of the chromatographic analyses. One of the authors (I.C.) thanks TUBITAK for the financial support by means of "Fellowships for Visiting Scientists and Scientists on Sabbatical Leave" programme.en_US
dc.identifier.doi10.1039/c6py01764a
dc.identifier.endpage7315en_US
dc.identifier.issn1759-9954
dc.identifier.issn1759-9962
dc.identifier.issn1759-9954en_US
dc.identifier.issn1759-9962en_US
dc.identifier.issue47en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage7304en_US
dc.identifier.urihttps://doi.org/10.1039/c6py01764a
dc.identifier.urihttps://hdl.handle.net/11454/53272
dc.identifier.volume7en_US
dc.identifier.wosWOS:000390555400015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofPolymer Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleSynthesis and application of a novel poly-L-phenylalanine electroactive macromonomer as matrix for the biosensing of 'Abused Drug' modelen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu