A generic model for diffusive dynamics of the substrate and fluorescein tagged enzyme in microfluidic platform

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dc.contributor.authorYildiz-Ozturk, Ece
dc.contributor.authorYucel, Mesut
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T10:04:43Z
dc.date.available2019-10-27T10:04:43Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to develop a model that describes enzymatic conversion in a microfluidic system along with convective and diffusive transport of substrate/product within the microchannels. Hence, a 1-D partial differential equation model based on Fick's Law was employed in order to describe the substrate/product concentration distributions within the system. Enzyme immobilized in PEO-inclusive/non-inclusive TEOS-based hydrogels were tagged with fluorescein and examined under confocal laser scanning microscopy to evaluate the distribution of the enzyme. The image processing analysis demonstrated that 88.2 +/- 5.9% of the PEO-inclusive gel fragment surface (pixelated) area was occupied by the enzyme-dye complex with an emission density of 29.92 +/- 1.07. Subsequent to ensuring the homogeneity throughout the microchannel, the effects of flow rate and the distance for a range of inlet substrate concentrations were considered in regards to various substrate concentrations and conversion rates. The developed model provided a quantification for the conversion of substrate into products and enhanced understanding of the transport phenomena in the hydrogel.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113M050]en_US
dc.description.sponsorshipWe acknowledge financial research support from the Scientific and Technical Research Council of Turkey (TUBITAK) 113M050 project. Additionally, we are grateful to Frank Bossler and Norbert Willenbacher for access to the Confocal Laser Scanning Microscopy at the Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology, Germany.en_US
dc.identifier.doi10.1007/s00542-018-3746-0
dc.identifier.endpage3105en_US
dc.identifier.issn0946-7076
dc.identifier.issn1432-1858
dc.identifier.issn0946-7076en_US
dc.identifier.issn1432-1858en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3095en_US
dc.identifier.urihttps://doi.org/10.1007/s00542-018-3746-0
dc.identifier.urihttps://hdl.handle.net/11454/30271
dc.identifier.volume24en_US
dc.identifier.wosWOS:000435585500023en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMicrosystem Technologies-Micro-And Nanosystems-Information Storage and Processing Systemsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA generic model for diffusive dynamics of the substrate and fluorescein tagged enzyme in microfluidic platformen_US
dc.typeArticleen_US

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