Treatment of immobilized ?-amylase under supercritical CO2 conditions: Can activity be enhanced after consecutive enzymatic reactions?

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dc.contributor.authorSenyay-Oncel D.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2019-10-27T08:23:32Z
dc.date.available2019-10-27T08:23:32Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractVarious approaches have been applied in order to enhance enzyme stability and activity. In this study, the aim was to investigate the capability of supercritical carbon dioxide (SC-CO2) to alter the activity of ?-amylase after consecutive enzymatic reactions. Therefore, ?-amylase from Aspergillus oryzae initially treated with SC-CO2 (29.7 mmol ml-1 min-1) was immobilized in Ca-alginate beads and NaY zeolite, subsequently assayed several times for the hydrolysis of soluble starch and when a lower activity value was recorded compared to the initial activity of the untreated enzyme (17.7 mmol ml-1 min -1), the immobilized samples were retreated with SC-CO2. These consecutive reactions and treatment loops were repeated till the activity cannot be increased with SC-CO2 retreatment in comparison to the initial activity of the untreated enzyme. The best results were achieved with NaY zeolite immobilized samples where four successful loops and seventeen reactions were realized till the residual activity. © 2013 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.molcatb.2013.03.003
dc.identifier.endpage76en_US
dc.identifier.issn1381-1177
dc.identifier.issn1381-1177en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage72en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2013.03.003
dc.identifier.urihttps://hdl.handle.net/11454/26443
dc.identifier.volume91en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Molecular Catalysis B: Enzymaticen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSupercritical fluid processsing Amylase Enzyme activity Immobilization Zeoliteen_US
dc.titleTreatment of immobilized ?-amylase under supercritical CO2 conditions: Can activity be enhanced after consecutive enzymatic reactions?en_US
dc.typeArticleen_US

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