Supercritical CO2 extraction of an immunosuppressant produced by solid-state fermentation

Basit Öğe Kaydı
dc.contributor.authorAlpak, Ilknur
dc.contributor.authorUzel, Ruhan Askin
dc.contributor.authorSargin, Sayit
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T10:02:06Z
dc.date.available2019-10-27T10:02:06Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractMycophenolic acid (MPA) is a secondary metabolite of Penicillium species with diverse biological properties and has clinical applications mainly as an immunosuppressive agent. Production of MPA was carried out using Penicillium brevicompactum (DSM 2215) by solid-state fermentation (SSF). Six different carbon sources (wheat bran, rice husk, rice bran, potato peel and rice husk - rice bran, rice bran-potato peel mixture) were examined by SSF in order to obtain the highest yield of mycophenolic acid. Rice bran-potato peel mixture was determined as the best solid substrate and used in scale up studies which was applied in the tray bioreactor. Supercritical CO2 and soxhlet extractions were analyzed for the recovery of MPA from the fermentation broth. The highest recovery (0.47 g MPA/100 g substrate) was obtained by supercritical CO2 at 30 MPa, 50 degrees C at a CO2 flow rate of 15 g/min with 10% ethanol as co-solvent for 3 h. The results obtained by the scale-up in bioreactor and optimization of supercritical CO2 entrained with ethanol can provide basis for the production of the immunosuppresant compound on industrial scale.en_US
dc.description.sponsorshipResearch Fund of Ege UniversityEge University [11MUH061]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of Ege University (11MUH061).en_US
dc.identifier.doi10.1016/j.jcou.2018.08.014
dc.identifier.endpage404en_US
dc.identifier.issn2212-9820
dc.identifier.issn2212-9839
dc.identifier.issn2212-9820en_US
dc.identifier.issn2212-9839en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage398en_US
dc.identifier.urihttps://doi.org/10.1016/j.jcou.2018.08.014
dc.identifier.urihttps://hdl.handle.net/11454/29948
dc.identifier.volume27en_US
dc.identifier.wosWOS:000445035500040en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal of Co2 Utilizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMycophenolic aciden_US
dc.subjectImmunosuppressanten_US
dc.subjectSupercritical CO2en_US
dc.subjectBioreactoren_US
dc.subjectScale-upen_US
dc.titleSupercritical CO2 extraction of an immunosuppressant produced by solid-state fermentationen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu