Endoplasmic reticulum stress regulates glutathione metabolism and activities of glutathione related enzymes in Arabidopsis

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dc.contributor.authorUzilday, Baris
dc.contributor.authorOzgur, Rengin
dc.contributor.authorSekmen, A. Hediye
dc.contributor.authorTurkan, Ismail
dc.date.accessioned2019-10-27T10:44:29Z
dc.date.available2019-10-27T10:44:29Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractStress conditions generate an extra load on protein folding machinery in the endoplasmic reticulum (ER) and if the ER cannot overcome this load, unfolded proteins accumulate in the ER lumen, causing ER stress. ER lumen localised protein disulfide isomerase (PDI) catalyses the generation of disulfide bonds in conjugation with ER oxidoreductase1 (ERO1) during protein folding. Mismatched disulfide bonds are reduced by the conversion of GSH to GSSG. Under prolonged ER stress, GSH pool is oxidised and H2O2 is produced via increased activity of PDI-ERO1. However, it is not known how glutathione metabolism is regulated under ER stress in plants. So, in this study, ER stress was induced with tunicamycin (0.15, 0.3, 0.45 mu g mL(-1) Tm) in Arabidopsis thaliana (L.) Heynh. Glutathione content was increased by ER stress, which was accompanied by induction of glutathione biosynthesis genes (GSH1, GSH2). Also, the apoplastic glutathione degradation pathway (GGT1) was induced. Further, the activities of glutathione reductase (GR), dehydroascorbate reductase (DHAR), glutathione peroxidase (GPX) and glutathione S-transferase (GST) were increased under ER stress. Results also showed that chloroplastic GPX genes were specifically downregulated with ER stress. This is the first report on regulation of glutathione metabolism and glutathione related enzymes in response to ER stress in plants.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [212T018]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant no. 212T018).en_US
dc.identifier.doi10.1071/FP17151
dc.identifier.endpage296en_US
dc.identifier.issn1445-4408
dc.identifier.issn1445-4416
dc.identifier.issn1445-4408en_US
dc.identifier.issn1445-4416en_US
dc.identifier.issue01.Feben_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage284en_US
dc.identifier.urihttps://doi.org/10.1071/FP17151
dc.identifier.urihttps://hdl.handle.net/11454/30984
dc.identifier.volume45en_US
dc.identifier.wosWOS:000419097200026en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCsiro Publishingen_US
dc.relation.ispartofFunctional Plant Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectER stressen_US
dc.subjecttunicamycinen_US
dc.subjectunfolded protein responseen_US
dc.titleEndoplasmic reticulum stress regulates glutathione metabolism and activities of glutathione related enzymes in Arabidopsisen_US
dc.typeArticleen_US

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