Determination of mitochondrial fragmentation and autophagosome formation in C2C12 skeletal muscle cells

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dc.contributor.authorTurkseven, Saadet
dc.contributor.authorZorzano, Antonio
dc.date.accessioned2019-10-27T21:42:47Z
dc.date.available2019-10-27T21:42:47Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: To investigate the effect of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on mitochondrial elimination in C2C12 skeletal muscle cells. Materials and methods: C2C12 muscle cells stably expressing Mito-DsRed were treated with DMSO or CCCP at the doses of 10, 20, and 30 mu M for 3, 6, and 24 h. After specific treatments, cells were fixed in 4% paraformaldehyde and immunocytochemistry assays were performed using appropriate antibodies to evaluate mitochondrial morphology and autophagosome formation. Total cell lysates were used for western blot assays. Results: CCCP induces mitochondrial fragmentation in C2C12 cells. In response to a mitochondrial uncoupler, endogenous autophagosome formation is significantly increased (P < 0.001). Co-localization analyses of confocal images indicate that fragmented mitochondria undergo engulfment by autophagosomes. Conclusion: The findings of this study will enhance our understanding of the mitophagic process in C2C12 skeletal muscle cells in order to identify molecular mechanisms governing removal of dysfunctional mitochondria.en_US
dc.identifier.doi10.3906/sag-1206-32
dc.identifier.endpage781en_US
dc.identifier.issn1300-0144
dc.identifier.issn1300-0144en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage775en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1206-32
dc.identifier.urihttps://hdl.handle.net/11454/46964
dc.identifier.volume43en_US
dc.identifier.wosWOS:000323610400017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMitophagyen_US
dc.subjectLC3en_US
dc.subjectskeletal muscle cellen_US
dc.titleDetermination of mitochondrial fragmentation and autophagosome formation in C2C12 skeletal muscle cellsen_US
dc.typeArticleen_US

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