cfDNA in exhaled breath condensate (EBC) and contamination by ambient air: toward volatile biopsies

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dc.contributor.authorKoc, Altug
dc.contributor.authorGoksel, Tuncay
dc.contributor.authorPelit, Levent
dc.contributor.authorKorba, Korcan
dc.contributor.authorDizdas, Tugberk N.
dc.contributor.authorBaysal, Ertan
dc.contributor.authorUzun, Umut C.
dc.contributor.authorKaya, Ozge Ozer
dc.contributor.authorOzyilmaz, Berk
dc.contributor.authorKutbay, Yasar B.
dc.contributor.authorOzdemir, Taha Resid
dc.contributor.authorKirbiyik, Ozgur
dc.contributor.authorErdogan, Kadri M.
dc.contributor.authorGuvenc, Merve Saka
dc.contributor.authorKocal, Gizem Calibasi
dc.contributor.authorBasbinar, Yasemin
dc.date.accessioned2019-10-27T09:43:27Z
dc.date.available2019-10-27T09:43:27Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractExhaled breath is a source of volatile and nonvolatile biomarkers in the body that can be accessed non-invasively and used for monitoring. The collection of lung secretions by conventional methods such as bronchoalveolar lavage, induced sputum collection, and core biopsies is limited by the invasive nature of these methods. Non-invasive collection of exhaled breath condensate (EBC) provides fluid samples that are representative of airway lining fluids. Various volatile and nonvolatile biomarkers can be detected in volatile condensates, such as H2O2, nitric oxide, lipid mediators, cytokines, chemokines, DNA, and microRNAs. Studies have examined cell-freeDNA(cfDNA) in plasma samples from non-small-cell lung cancer patients, offering to new insights and fostering development of the liquid biopsy. However, few studies have examined cfDNA in EBC samples. This study examined whether EBC is an appropriate source of cfDNA using housekeeping-gene-specific primer probes and quantitative real-time polymerase chain reaction in healthy subjects. Ambient (room) air is contaminated with DNA, so caution is needed. Preliminary studies indicated that volatile biopsies are becoming an important diagnostic tool in lung cancer.en_US
dc.description.sponsorshipDepartment of Scientific Research Projects of Dokuz Eylul University [KB.SAG.049]; Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [216S435, 216S591]en_US
dc.description.sponsorshipInformed consent was obtained from all research subjects. The study was approved by the Ethics Committee of Ege University and was supported by grants from the Department of Scientific Research Projects of Dokuz Eylul University (Project no: 2017.KB.SAG.049) and the Scientific and Technological Research Council of Turkey (Project no: 216S435 and 216S591).en_US
dc.identifier.doi10.1088/1752-7163/ab17ff
dc.identifier.issn1752-7155
dc.identifier.issn1752-7163
dc.identifier.issn1752-7155en_US
dc.identifier.issn1752-7163en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1088/1752-7163/ab17ff
dc.identifier.urihttps://hdl.handle.net/11454/28871
dc.identifier.volume13en_US
dc.identifier.wosWOS:000466805600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIop Publishing Ltden_US
dc.relation.ispartofJournal of Breath Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcfDNAen_US
dc.subjectexhaled breath condensateen_US
dc.subjectvolatile condensateen_US
dc.titlecfDNA in exhaled breath condensate (EBC) and contamination by ambient air: toward volatile biopsiesen_US
dc.typeArticleen_US

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