Differential gene expression analysis of human cumulus cells

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dc.contributor.authorDemiray S.B.
dc.contributor.authorGoker E.N.T.
dc.contributor.authorTavmergen E.
dc.contributor.authorYilmaz O.
dc.contributor.authorCalimlioglu N.
dc.contributor.authorSoykam H.O.
dc.contributor.authorOktem G.
dc.contributor.authorSezerman U.
dc.date.accessioned2019-10-26T21:12:16Z
dc.date.available2019-10-26T21:12:16Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods: Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results: Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p-value and highest number of times found count. Conclusion: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy. © 2019. The Korean Society for Reproductive Medicine.en_US
dc.description.sponsorshipFirat University Scientific Research Projects Management Unit, FÃ?BAP: Ege-BAP TIP-00014en_US
dc.description.sponsorship*This work was supported by the University Scientific Research Project Fund (Ege-BAP TIP-00014, 2011). --en_US
dc.identifier.doi10.5653/cerm.2019.46.2.76
dc.identifier.endpage86en_US
dc.identifier.issn2233-8233
dc.identifier.issn2233-8233en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage76en_US
dc.identifier.urihttps://doi.org/10.5653/cerm.2019.46.2.76
dc.identifier.urihttps://hdl.handle.net/11454/15654
dc.identifier.volume46en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKorean Society for Reproductive Medicineen_US
dc.relation.ispartofClinical and Experimental Reproductive Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAssisted reproductive techniquesen_US
dc.subjectCircadian clocksen_US
dc.subjectCumulus cellsen_US
dc.subjectGranulosa cellsen_US
dc.subjectInfertilityen_US
dc.subjectLive birthen_US
dc.subjectMicroarray analysisen_US
dc.subjectOvarian follicleen_US
dc.subjectPregnancyen_US
dc.subjectProteoglycansen_US
dc.titleDifferential gene expression analysis of human cumulus cellsen_US
dc.typeArticleen_US

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