Characterization of Changes due to pH Variations in Beta Peptide ((25-35)) Leading to Alzheimer's Disease

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dc.contributor.authorSenthil, Rethinam
dc.contributor.authorSrividya, S.
dc.contributor.authorAruni, A. Wilson
dc.contributor.authorBasaran, Bahri
dc.contributor.authorJyotsna
dc.contributor.authorThirugnanasambandam, Rajendran
dc.date.accessioned2020-12-01T12:06:17Z
dc.date.available2020-12-01T12:06:17Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractThe effect of various concentrations of amyloid beta peptide (ABP) in different pH (pH 2, 6, 7, 8, 10) in aging at different time intervals was analysed using various techniques. the pH 7.4 and 8 were compared with other pH solution which indicated that the secondary structure content with high beta-sheet conformation is found at pH 7.4 and 8. Phosphate buffer solutions (pH) were detected using CD spectroscopy. the CD spectra of peptide in phosphate buffer (pH 2 and 10) on aging at time intervals were also analysed with the aging of sample (pH 2) on the 10th day. the results showed no major secondary structural changes in the peptide, which was indicative predominant random coil structure. the phosphate buffer pH 6, 7.4, 8 on the 10th day showed the occurrence of crossover point. This same sample smear on slide was mounded and the fibrils were visualized using the light microscope. the percent of viable cell in different pH was detected in MTT (3-(4,5-Dimethyazol-2-yl)-2, 5-diphenyl-tetrazolium bromide) assay using human keratinocyte cell line (HaCaT). Fluorescence spectroscopy was used to study the growth and development of fibrils.en_US
dc.identifier.doi10.1007/s10989-019-09987-0
dc.identifier.endpage1870en_US
dc.identifier.issn1573-3149
dc.identifier.issn1573-3904
dc.identifier.issn1573-3149en_US
dc.identifier.issn1573-3904en_US
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85075623493en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1863en_US
dc.identifier.urihttps://doi.org/10.1007/s10989-019-09987-0
dc.identifier.urihttps://hdl.handle.net/11454/63194
dc.identifier.volume26en_US
dc.identifier.wosWOS:000498986900001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofInternational Journal of Peptide Research and Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPeptide-A beta ((25-35)) Alzheimer's diseaseen_US
dc.subjectCD spectroscopyen_US
dc.subjectFluorescence spectroscopyen_US
dc.subjectMTT assayen_US
dc.titleCharacterization of Changes due to pH Variations in Beta Peptide ((25-35)) Leading to Alzheimer's Diseaseen_US
dc.typeArticleen_US

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