Identification of proteins involved in excess boron stress in roots of carrot (Daucus carota L.) and role of niacin in the protein profiles

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dc.contributor.authorDemiray, Hatice
dc.contributor.authorDereboylu, Aylin E.
dc.contributor.authorAltan, Filiz
dc.contributor.authorZeytunluoglu, Ali
dc.date.accessioned2019-10-27T21:35:26Z
dc.date.available2019-10-27T21:35:26Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractPlants are constantly challenged by various biotic and abiotic stresses in nature. Boron toxicity have become one of the important abiotic stress factor for plants. Boron toxicity responses of plants is reflected by alterations in protein expression level, activity, location and concentration. In this study, we identified the proteins which respond to the boron excess stress in Daucus carota root cells, and examined the effects of niacin (nicotinamide) on the protein profiles of boron excess. Protein profile changes in responce to boron and niacin were compared with the control using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gel electrophoresis. Protein bands of interest were excised from gel and digested by trypsin. The trypsin-digested peptides were analyzed on nanoLC-ESI-MS/MS. Six different proteins involved in plant defense system were identified in total [CR16 (Major allergen Dau c1), glutathion peroxidase, glyoxylase I, isocitrate dehydrogenase (NADP(+)), adenosyl homocysteinase, EDPG precursor)]. Our results clearly demonstrated the presence of boron and niacin in growth medium stimulated expression and synthesis of proteins role in plant defence mechanism.en_US
dc.identifier.doi10.5897/AJB11.1117
dc.identifier.endpage15551en_US
dc.identifier.issn1684-5315
dc.identifier.issn1684-5315en_US
dc.identifier.issue69en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage15545en_US
dc.identifier.urihttps://doi.org/10.5897/AJB11.1117
dc.identifier.urihttps://hdl.handle.net/11454/45861
dc.identifier.volume10en_US
dc.identifier.wosWOS:000298555400012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBoronen_US
dc.subjectniacinen_US
dc.subjectDaucus carotaen_US
dc.subjectplant defence enzymesen_US
dc.titleIdentification of proteins involved in excess boron stress in roots of carrot (Daucus carota L.) and role of niacin in the protein profilesen_US
dc.typeArticleen_US

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