Synthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugation

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dc.contributor.authorEkiz, Fulya
dc.contributor.authorOguzkaya, Funda
dc.contributor.authorAkin, Mehriban
dc.contributor.authorTimur, Suna
dc.contributor.authorTanyeli, Cihangir
dc.contributor.authorToppare, Levent
dc.date.accessioned2019-10-27T21:25:48Z
dc.date.available2019-10-27T21:25:48Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractHere we report the synthesis of a novel conducting polymer and its properties as an immobilization platform for biosensor application. The conducting polymer has functional groups used for the formation of amide bonding with the enzyme immobilized on the polymer surface. After covalent immobilization of glucose oxidase (GOx) on the polymeric matrix, its application for glucose biosensing was investigated in detail. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements were used to monitor the surface properties of the polymer before and after biomolecule conjugation. The optimized biosensor showed a very good linearity between 0.01 mM and 1.2 mM, a 13 s response time and a detection limit (LOD) of 0.004 mM to glucose. Also, kinetic parameters, operational and storage stabilities were determined. Apparent Michaelis constant (K-m(app)) and I-max values of 1.17 mM and 11.28 mu A, respectively, were obtained.en_US
dc.description.sponsorshipTUBATurkish Academy of Sciences; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110T580]en_US
dc.description.sponsorshipThis work is partially supported by TUBA and TUBITAK 110T580 grants. METU Central Laboratory is acknowledged for the SEM images. Merve Yuksel is gratefully acknowledged for her contributions in HPLC applications. Dr Selda Keskin is also gratefully acknowledged for access to the XPS facilities.en_US
dc.identifier.doi10.1039/c1jm12048d
dc.identifier.endpage12343en_US
dc.identifier.issn0959-9428
dc.identifier.issn1364-5501
dc.identifier.issn0959-9428en_US
dc.identifier.issn1364-5501en_US
dc.identifier.issue33en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage12337en_US
dc.identifier.urihttps://doi.org/10.1039/c1jm12048d
dc.identifier.urihttps://hdl.handle.net/11454/44875
dc.identifier.volume21en_US
dc.identifier.wosWOS:000293694700027en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofJournal of Materials Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleSynthesis and application of poly-SNS-anchored carboxylic acid: a novel functional matrix for biomolecule conjugationen_US
dc.typeArticleen_US

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