Double fluorescence assay via a beta-cyclodextrin containing conjugated polymer as a biomimetic material for cocaine sensing

Basit Öğe Kaydı
dc.contributor.authorArslan, Mustafa
dc.contributor.authorSengel, Tulay Yilmaz
dc.contributor.authorGuler, Emine
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorAldemir, Ebru
dc.contributor.authorAkbulut, Huseyin
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorTimur, Suna
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-10-27T11:07:27Z
dc.date.available2019-10-27T11:07:27Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractA double fluorescence mu-well assay that exploits a novel conjugated polymer containing cyclodextrin (CD) as the key component is reported. For the construction of the cocaine bioassay, poly(p-phenylene) with CD units in the main-chain and poly(ethylene glycol) side chains (PPP-CD-g-PEG) was first prepared by Suzuki coupling polymerization and coated on each well as a biomimetic material. Although the poly-phenylene backbone is responsible for the fluorescence properties without an additional fluorophore, PEG and CD provide water solubility and selective complexation with cocaine, respectively. A cocaine antibody was used as a secondary recognition compound after labelling with quantum dots (QDs). Most notably, we show that the two-color fluorescence nature of the assay facilitates double measurement from the same sample and the described strategy can be adapted to various sensing systems.en_US
dc.description.sponsorshipRepublic of Turkey, Ministry of Science, Industry and TechnologyMinistry of Science, Industry & Technology - Turkey [0620.STZ.2014]; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016 K121190]; Ege University Research Fund through the BAP [16-FEN-004]; Istanbul Technical University Research Funden_US
dc.description.sponsorshipThis work was supported by the Industrial Thesis Support Program (SAN-TEZ) of the Republic of Turkey, Ministry of Science, Industry and Technology (Project Grant No. 0620.STZ.2014) and the Republic of Turkey, Ministry of Development (Project Grant No. 2016 K121190). This work was also partially supported by the Ege University Research Fund through the BAP project (Project No. 16-FEN-004) and the Istanbul Technical University Research Fund. Likrom Company was acknowledged for its support to the chromatographic analyses.en_US
dc.identifier.doi10.1039/c7py00420f
dc.identifier.endpage3340en_US
dc.identifier.issn1759-9954
dc.identifier.issn1759-9962
dc.identifier.issn1759-9954en_US
dc.identifier.issn1759-9962en_US
dc.identifier.issue21en_US
dc.identifier.startpage3333en_US
dc.identifier.urihttps://doi.org/10.1039/c7py00420f
dc.identifier.urihttps://hdl.handle.net/11454/32029
dc.identifier.volume8en_US
dc.identifier.wosWOS:000402300400012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofPolymer Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleDouble fluorescence assay via a beta-cyclodextrin containing conjugated polymer as a biomimetic material for cocaine sensingen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu