Peptide-modified conducting polymer as a biofunctional surface: monitoring of cell adhesion and proliferation

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dc.contributor.authorOyman, Gizem
dc.contributor.authorGeyik, Caner
dc.contributor.authorAyranci, Rukiye
dc.contributor.authorAk, Metin
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorTimur, Suna
dc.contributor.authorCoskunol, Hakan
dc.date.accessioned2019-10-27T22:06:29Z
dc.date.available2019-10-27T22:06:29Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractHere, we report the electropolymerization of 3-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer on indium tin oxide (ITO) glass and its use as a coating material for cell culture applications. Functional amino groups on the conducting polymer provide post-modification of the surface with the arginylglycylaspartic acid (RGD) peptide via EDC chemistry. Scanning electron microscopy, atomic force microscopy, and contact angle and surface conductivity measurements were carried out for the surface characterization. The peptide-conjugated surface was tested for adhesion and proliferation of several cell lines such as monkey kidney epithelial (Vero), human neuroblastoma (SH-SY5Y), and human immortalized skin keratinocyte (HaCaT). These cells were cultured on RGD-modified, polymer-coated ITO glass as well as conventional polystyrene surfaces for comparison. The data indicate that the RGD-modified surfaces exhibited better cell adhesion and proliferation among all surfaces compared. Cell imaging studies up to 72 h in length were performed on these surfaces using different microscopy techniques. Therefore, the novel biofunctional substrate is a promising candidate for further studies such as monitoring the effects of drugs and chemicals on cellular viability and morphology as well as cell-culture-on-a-chip applications.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z918]; Ege University Research FoundationEge University [12-TIP-104, 14-FEN-023]en_US
dc.description.sponsorshipThis project was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, project number 113Z918) and the Ege University Research Foundation (project numbers 12-TIP-104 and 14-FEN-023). METU Central Laboratory is acknowledged for the contact angle and AFM analyses. We thank IYTE MAM for SEM analyses. The authors also thank Prof. Dr S. Sakarya (Adnan Menderes University) and Prof. Dr H. O. Sercan (Dokuz Eylul University) for their support.en_US
dc.identifier.doi10.1039/c4ra08481k
dc.identifier.endpage53418en_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069en_US
dc.identifier.issue96en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage53411en_US
dc.identifier.urihttps://doi.org/10.1039/c4ra08481k
dc.identifier.urihttps://hdl.handle.net/11454/48813
dc.identifier.volume4en_US
dc.identifier.wosWOS:000344468800006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titlePeptide-modified conducting polymer as a biofunctional surface: monitoring of cell adhesion and proliferationen_US
dc.typeArticleen_US

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